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XPF (ERCC4) (NM_005236) Human Tagged ORF Clone
CAT＃: RC223300
ERCC4 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4)
ORF Plasmid: tGFP
"NM_005236" in other vectors (6)
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CNY 6,712.00
CNY 7,130.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ERCC11; FANCQ; RAD1; XFEPS; XPF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223300 representing NM_005236
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGGGCAGCCGGCTCGACGGATTGCCATGGCGCCGCTGCTGGAGTACGAGCGACAGCTGGTGC TGGAACTGCTCGACACTGACGGGCTAGTAGTGTGCGCCCGCGGGCTCGGCGCGGACCGGCTCCTCTACCA CTTTCTCCAGCTGCACTGCCACCCAGCCTGCCTGGTGCTGGTGCTCAACACGCAGCCGGCCGAGGAGGAG TATTTTATCAATCAGCTGAAGATAGAAGGAGTTGAACACCTCCCTCGCCGTGTAACAAATGAAATCACAA GCAACAGTCGCTATGAAGTTTACACACAAGGTGGTGTTATATTTGCGACAAGTAGGATACTTGTGGTTGA CTTCTTGACTGATAGAATACCTTCAGATTTAATTACTGGCATCTTGGTGTATAGAGCCCACAGAATAATC GAGTCTTGTCAAGAAGCATTCATCTTGCGCCTCTTTCGCCAGAAAAACAAACGTGGTTTTATTAAAGCTT TCACAGACAATGCTGTTGCCTTTGATACTGGTTTTTGTCATGTGGAAAGAGTGATGAGAAATCTTTTTGT GAGGAAACTGTATCTGTGGCCAAGGTTCCATGTAGCAGTAAACTCATTTTTAGAACAGCACAAACCTGAA GTTGTAGAAATCCATGTTTCTATGACACCTACCATGCTTGCTATACAGACTGCTATACTGGACATTTTAA ATGCATGTCTAAAGGAACTAAAATGCCATAACCCATCGCTTGAAGTGGAAGATTTATCTTTAGAAAATGC TATTGGAAAACCTTTTGACAAGACAATCCGCCATTATCTGGATCCTTTGTGGCACCAGCTTGGAGCCAAG ACTAAATCCTTAGTTCAGGATTTGAAGATATTACGAACTTTGCTGCAGTATCTCTCTCAGTATGATTGTG TCACATTTCTTAATCTTCTGGAATCTCTGAGAGCAACGGAAAAAGCTTTTGGTCAGAATTCAGGTTGGCT GTTTCTTGACTCCAGCACCTCGATGTTTATAAATGCTCGAGCAAGGGTTTATCATCTTCCAGATGCCAAA ATGAGTAAAAAAGAAAAAATATCTGAAAAAATGGAAATTAAAGAAGGGGAAGAAACAAAAAAGGAACTGG TCCTAGAAAGCAACCCAAAGTGGGAGGCACTGACTGAAGTATTAAAAGAAATTGAGGCAGAAAATAAGGA GAGTGAAGCTCTTGGTGGTCCAGGTCAAGTACTGATTTGTGCAAGTGATGACCGAACATGTTCCCAGCTG AGAGACTATATCACTCTTGGAGCGGAGGCCTTCTTATTGAGGCTCTACAGGAAAACCTTTGAGAAGGATA GCAAAGCTGAAGAAGTCTGGATGAAATTTAGGAAGGAAGACAGTTCAAAGAGAATTAGGAAATCTCACAA AAGACCTAAAGACCCCCAAAACAAAGAACGGGCTTCTACCAAAGAAAGAACCCTCAAAAAGAAAAAACGG AAGTTGACCTTAACTCAAATGGTAGGAAAACCTGAAGAACTGGAAGAGGAAGGAGATGTCGAGGAAGGAT ATCGTCGAGAAATAAGCAGTAGCCCAGAAAGCTGCCCGGAAGAAATTAAGCATGAAGAATTTGATGTAAA TTTGTCATCGGATGCTGCTTTCGGAATCCTGAAAGAACCCCTCACTATCATCCATCCGCTTCTGGGTTGC AGCGACCCCTATGCTCTGACAAGGGTACTACATGAAGTGGAGCCAAGATACGTGGTTCTTTATGACGCAG AGCTAACCTTTGTTCGGCAGCTTGAAATTTACAGGGCGAGTAGGCCTGGGAAACCTCTGAGGGTTTACTT TCTTATATACGGAGGTTCAACTGAGGAACAACGCTATCTCACTGCTTTGCGGAAAGAAAAGGAAGCTTTT GAAAAACTCATAAGGGAAAAAGCAAGCATGGTTGTCCCTGAAGAAAGAGAAGGCAGAGATGAAACAAACT TAGACCTAGTAAGAGGCACAGCATCTGCAGATGTTTCCACTGACACTCGGAAAGCCGGTGGCCAGGAACA GAATGGTACACAGCAAAGCATAGTTGTGGATATGCGTGAATTTCGAAGTGAGCTTCCATCTCTGATCCAT CGTCGGGGCATTGACATTGAACCCGTGACTTTAGAGGTTGGAGATTACATCCTCACTCCAGAAATGTGCG TGGAGCGCAAGAGTATCAGTGATTTAATCGGCTCTTTAAATAACGGCCGCCTCTACAGCCAGTGCATCTC CATGTCCCGCTACTACAAGCGTCCCGTGCTTCTGATTGAGTTTGACCCTAGCAAGCCTTTCTCTCTCACT TCCCGAGGTGCCTTGTTTCAGGAGATCTCCAGCAATGACATTAGTTCCAAACTCACTCTTCTTACACTTC ACTTCCCCAGACTACGGATTCTCTGGTGCCCCTCTCCTCATGCAACGGCGGAGTTGTTTGAGGAGCTGAA ACAAAGCAAGCCACAGCCTGATGCGGCGACAGCACTGGCCATTACAGCAGATTCCGAAACCCTTCCCGAG TCAGAGAAGTATAATCCTGGTCCCCAAGACTTCTTGTTAAAAATGCCAGGGGTGAATGCCAAAAACTGCC GCTCCTTGATGCACCACGTTAAGAACATCGCAGAATTAGCAGCCCTGTCACAAGACGAGCTCACGAGTAT TCTGGGGAATGCTGCAAATGCCAAACAGCTTTATGATTTCATTCACACCTCTTTTGCAGAAGTCGTATCA AAAGGAAAAGGGAAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223300 representing NM_005236
Red=Cloning site Green=Tags(s) MESGQPARRIAMAPLLEYERQLVLELLDTDGLVVCARGLGADRLLYHFLQLHCHPACLVLVLNTQPAEEE YFINQLKIEGVEHLPRRVTNEITSNSRYEVYTQGGVIFATSRILVVDFLTDRIPSDLITGILVYRAHRII ESCQEAFILRLFRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPE VVEIHVSMTPTMLAIQTAILDILNACLKELKCHNPSLEVEDLSLENAIGKPFDKTIRHYLDPLWHQLGAK TKSLVQDLKILRTLLQYLSQYDCVTFLNLLESLRATEKAFGQNSGWLFLDSSTSMFINARARVYHLPDAK MSKKEKISEKMEIKEGEETKKELVLESNPKWEALTEVLKEIEAENKESEALGGPGQVLICASDDRTCSQL RDYITLGAEAFLLRLYRKTFEKDSKAEEVWMKFRKEDSSKRIRKSHKRPKDPQNKERASTKERTLKKKKR KLTLTQMVGKPEELEEEGDVEEGYRREISSSPESCPEEIKHEEFDVNLSSDAAFGILKEPLTIIHPLLGC SDPYALTRVLHEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAF EKLIREKASMVVPEEREGRDETNLDLVRGTASADVSTDTRKAGGQEQNGTQQSIVVDMREFRSELPSLIH RRGIDIEPVTLEVGDYILTPEMCVERKSISDLIGSLNNGRLYSQCISMSRYYKRPVLLIEFDPSKPFSLT SRGALFQEISSNDISSKLTLLTLHFPRLRILWCPSPHATAELFEELKQSKPQPDAATALAITADSETLPE SEKYNPGPQDFLLKMPGVNAKNCRSLMHHVKNIAELAALSQDELTSILGNAANAKQLYDFIHTSFAEVVS KGKGKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005236 |
| ORF Size | 2748 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005236.3 |
| RefSeq Size | 2751 bp |
| RefSeq ORF | 2751 bp |
| Locus ID | 2072 |
| UniProt ID | Q92889 |
| Protein Families | Druggable Genome |
| Protein Pathways | Nucleotide excision repair |
| MW | 104.3 kDa |
| Gene Summary | The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).[provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223300L1 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), Myc-DDK-tagged |
CNY 9,112.00 |
|
| RC223300L2 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), mGFP tagged |
CNY 9,112.00 |
|
| RC223300L3 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), Myc-DDK-tagged |
CNY 9,112.00 |
|
| RC223300L4 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), mGFP tagged |
CNY 9,030.00 |
|
| RG223300 | ERCC4 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) |
CNY 8,312.00 |
|
| SC314914 | ERCC4 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) |
CNY 6,720.00 |
