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WNK4 (NM_032387) Human Tagged ORF Clone
CAT＃: RC223269
WNK4 (Myc-DDK-tagged)-Human WNK lysine deficient protein kinase 4 (WNK4)
ORF Plasmid: tGFP
"NM_032387" in other vectors (6)
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CNY 8,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PHA2B; PRKWNK4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223269 representing NM_032387
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGCATCCCCGGCCACGGAGACCACCGTCCTCATGTCCCAGACTGAGGCCGACCTGGCCCTGCGGC CCCCGCCTCCTCTTGGCACCGCGGGGCAGCCCCGCCTCGGGCCCCCTCCTCGCCGAGCGCGCCGCTTCTC CGGGAAGGCTGAGCCCCGGCCGCGCTCTTCTCGTCTCAGCCGCCGTAGCTCAGTCGACTTGGGGCTGCTG AGCTCTTGGTCCCTGCCAGCCTCACCCGCTCCGGACCCCCCCGATCCTCCGGACTCCGCTGGTCCTGGCC CCGCGAGGAGCCCACCGCCTAGCTCCAAAGAACCCCCCGAGGGCACGTGGACCGAGGGAGCCCCTGTGAA GGCTGCGGAAGACTCCGCGCGTCCCGAGCTCCCGGACTCTGCAGTGGGCCCGGGGTCCAGGGAGCCGCTA AGGGTCCCTGAAGCTGTGGCCCTAGAGCGGCGGCGGGAGCAGGAAGAAAAGGAGGACATGGAGACCCAGG CTGTGGCAACGTCCCCCGATGGCCGATACCTCAAGTTTGACATCGAGATTGGACGTGGCTCCTTCAAGAC GGTGTATCGAGGGCTAGACACCGACACCACAGTGGAGGTGGCCTGGTGTGAGCTGCAGACTCGGAAACTG TCTAGAGCTGAGCGGCAGCGCTTCTCAGAGGAGGTGGAGATGCTCAAGGGGCTGCAGCACCCCAACATCG TCCGCTTCTATGATTCGTGGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTCACCGAACTCAT GACCTCGGGCACGCTCAAGACGTACCTGAGGCGGTTCCGGGAGATGAAGCCGCGGGTCCTTCAGCGCTGG AGCCGCCAAATCCTGCGGGGACTTCATTTCCTACACTCCCGGGTTCCTCCCATCCTGCACCGGGATCTCA AGTGCGACAATGTCTTTATCACGGGACCTACTGGCTCTGTCAAAATCGGGGACCTGGGCCTGGCCACGCT CAAGCGCGCCTCCTTTGCCAAGAGTGTCATCGGGACCCCGGAATTCATGGCCCCCGAGATGTACGAGGAA AAGTACGATGAGGCCGTGGACGTGTACGCGTTCGGCATGTGCATGCTGGAGATGGCCACCTCTGAGTACC CGTACTCCGAGTGCCAGAATGCCGCGCAAATCTACCGCAAGGTCACTTCGGGCAGAAAGCCGAACAGCTT CCACAAGGTGAAGATACCCGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAACGAGAGG TTCACCATCCAGGACCTCCTGGCCCACGCCTTCTTCCGCGAGGAGCGCGGTGTGCACGTGGAACTAGCGG AGGAGGACGACGGCGAGAAGCCGGGCCTCAAGCTCTGGCTGCGCATGGAGGACGCGCGGCGCGGGGGGCG CCCACGGGACAACCAGGCCATCGAGTTCCTGTTCCAGCTGGGCCGGGACGCGGCCGAGGAGGTGGCACAG GAGATGGTGGCTCTGGGCTTGGTCTGTGAAGCCGATTACCAGCCAGTGGCCCGTGCAGTACGTGAACGGG TTGCTGCCATCCAGCGAAAGCGTGAGAAGCTGCGTAAAGCAAGGGAATTGGAGGCACTCCCACCAGAGCC AGGACCTCCACCAGCAACTGTGCCCATGGCCCCCGGTCCCCCCAGTGTCTTCCCCCCTGAGCCTGAGGAG CCAGAGGCAGACCAGCACCAGCCCTTCCTTTTCCGCCACGCCAGCTACTCATCTACCACTTCGGATTGCG AGACTGATGGCTACCTCAGCTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCTGGGGG GGTGCCATCCAGCCTGGCTGAGTCCCATCTCTGCCTGCCCTCGGCTTTTGCCCTATCCATTCCACGTTCT GGCCCTGGAAGTGACTTTTCCCCCGGGGACAGCTATGCCTCAGATGCAGCTTCAGGCCTTAGCGATGTGG GAGAAGGGATGGGACAAATGAGGAGACCCCCAGGGAGGAATCTCCGGCGCAGACCCCGATCCCGGCTGCG GGTCACTAGTGTCTCAGACCAGAATGACAGAGTGGTTGAGTGCCAGCTACAGACCCATAACAGCAAGATG GTGACCTTCCGATTTGATCTGGATGGGGACAGCCCGGAAGAGATTGCAGCTGCCATGGTATATAACGAGT TCATTCTGCCTTCGGAGCGAGATGGATTTCTCAGACGGATTCGGGAGATTATCCAGCGAGTGGAGACCCT GTTGAAGAGAGACACTGGCCCCATGGAGGCTGCTGAAGACACCCTAAGCCCCCAGGAGGAGCCAGCACCA TTACCTGCCCTGCCCGTCCCCCTCCCAGACCCATCCAATGAAGAGCTCCAGAGCAGCACCTCCCTGGAGC ACAGGAGCTGGACAGCCTTCTCCACCTCCTCATCTTCTCCTGGAACTCCTTTGTCTCCTGGAAACCCATT TTCCCCTGGAACCCCCATTTCCCCAGGTCCCATCTTCCCCATCACTTCTCCCCCATGTCATCCCAGCCCC TCCCCATTCTCCCCCATTTCTTCCCAGGTCTCCTCAAATCCCTCTCCACACCCCACCAGCTCTCCACTTC CATTCTCCTCCAGCACACCCGAGTTTCCGGTCCCACTCTCTCAGTGTCCCTGGAGTTCTCTCCCCACGAC TTCTCCACCTACGTTCTCTCCCACTTGTTCTCAGGTCACTCTTAGTTCCCCTTTCTTTCCTCCGTGCCCC TCCACTTCTTCCTTCCCCTCCACCACAGCAGCCCCTCTCCTTTCTCTGGCTAGTGCCTTCTCACTGGCTG TGATGACTGTGGCCCAGTCCCTGCTGTCCCCCTCACCTGGGCTCCTTTCCCAGTCTCCTCCAGCCCCTCC TAGTCCCCTCCCTAGCCTGCCCCTTCCCCCTCCCGTTGCTCCTGGTGGCCAGGAAAGCCCTTCACCCCAC ACAGCTGAGGTGGAGAGTGAGGCCTCACCACCTCCTGCTCGGCCCCTCCCAGGGGAAGCCAGGCTGGCGC CCATCTCTGAAGAGGGAAAGCCGCAGCTTGTTGGGCGTTTCCAAGTGACTTCATCCAAGGAACCGGCTGA GCCTCTTCCCTTGCAGCCAACATCCCCCACTCTCTCTGGTTCTCCAAAACCTTCAACCCCTCAGCTCACT TCAGAGAGCTCAGATACAGAGGACAGTGCTGGAGGCGGGCCAGAGACCAGGGAAGCTCTGGCTGAGAGCG ACCGTGCAGCTGAGGGTCTGGGGGCTGGAGTTGAGGAGGAAGGAGATGATGGGAAGGAACCCCAAGTTGG GGGCAGCCCCCAACCCCTGAGCCATCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCTGTGTTTG AGCAGCGAGGAGTCAGAAAGCAGTGGGGAAGATGAGGAGTTCTGGGCTGAGCTGCAGAGTCTTCGGCAGA AGCACTTGTCAGAGGTGGAAACACTACAGACACTACAGAAAAAAGAAATTGAAGATTTGTACAGCCGGCT GGGGAAGCAGCCCCCACCGGGTATTGTGGCCCCAGCTGCTATGCTGTCCAGCCGCCAGCGCCGCCTCTCC AAGGGCAGCTTCCCCACCTCCCGCCGCAACAGCCTACAGCGCTCTGAGCCCCCAGGCCCTGGCATCATGC GAAGGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGACATTCGC CGGGGATGTTGGCAGGATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223269 representing NM_032387
Red=Cloning site Green=Tags(s) MLASPATETTVLMSQTEADLALRPPPPLGTAGQPRLGPPPRRARRFSGKAEPRPRSSRLSRRSSVDLGLL SSWSLPASPAPDPPDPPDSAGPGPARSPPPSSKEPPEGTWTEGAPVKAAEDSARPELPDSAVGPGSREPL RVPEAVALERRREQEEKEDMETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKL SRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRW SRQILRGLHFLHSRVPPILHRDLKCDNVFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEE KYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGRKPNSFHKVKIPEVKEIIEGCIRTDKNER FTIQDLLAHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQ EMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPEPGPPPATVPMAPGPPSVFPPEPEE PEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGVPSSLAESHLCLPSAFALSIPRS GPGSDFSPGDSYASDAASGLSDVGEGMGQMRRPPGRNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKM VTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLRRIREIIQRVETLLKRDTGPMEAAEDTLSPQEEPAP LPALPVPLPDPSNEELQSSTSLEHRSWTAFSTSSSSPGTPLSPGNPFSPGTPISPGPIFPITSPPCHPSP SPFSPISSQVSSNPSPHPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTFSPTCSQVTLSSPFFPPCP STSSFPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPSPLPSLPLPPPVAPGGQESPSPH TAEVESEASPPPARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLPLQPTSPTLSGSPKPSTPQLT SESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEGDDGKEPQVGGSPQPLSHPSPVWMNYSYSSLCL SSEESESSGEDEEFWAELQSLRQKHLSEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLS KGSFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032387 |
| ORF Size | 3729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032387.5 |
| RefSeq Size | 4147 bp |
| RefSeq ORF | 3732 bp |
| Locus ID | 65266 |
| UniProt ID | Q96J92 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 134.6 kDa |
| Gene Summary | This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223269L1 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), Myc-DDK-tagged |
CNY 11,056.00 |
|
| RC223269L2 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), mGFP tagged |
CNY 14,350.00 |
|
| RC223269L3 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC223269L4 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), mGFP tagged |
CNY 14,350.00 |
|
| RG223269 | WNK4 (tGFP-tagged) - Human WNK lysine deficient protein kinase 4 (WNK4) |
CNY 13,680.00 |
|
| SC305438 | WNK4 (untagged)-Human WNK lysine deficient protein kinase 4 (WNK4) |
CNY 10,450.00 |
