ALDH1A2 (NM_003888) Human Tagged ORF Clone

CAT#: RC223250

  • TrueORF®

ALDH1A2 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member A2 (ALDH1A2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_003888" in other vectors (6)

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CNY 3,856.00

CNY 4,470.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Anti-ALDH1A2 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RALDH(II); RALDH2; RALDH2-T
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223250 representing NM_003888
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCCAGCAAGATAGAGATGCCCGGCGAGGTGAAGGCCGACCCCGCCGCCCTCATGGCGTCGCTGC
ACCTCCTGCCGTCGCCCACGCCCAATCTCGAAATTAAGTACACCAAGATCTTTATAAACAACGAGTGGCA
GAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTATAATCCAGCCACAGGAGAACAGGTGTGTGAAGTTCAA
GAAGCAGACAAGGCAGATATAGACAAAGCAGTGCAGGCAGCCCGCCTGGCTTTCTCTCTTGGTTCAGTGT
GGAGAAGGATGGATGCTTCAGAAAGGGGACGTCTGTTGGATAAGCTTGCAGACTTGGTGGAACGGGACAG
GGCAGTTCTTGCAACCATGGAATCCCTAAATGGTGGCAAACCATTCCTGCAAGCTTTTTATGTGGATTTG
CAGGGCGTCATCAAAACCTTTCGATATTACGCAGGCTGGGCTGATAAAATTCATGGGATGACCATTCCTG
TAGATGGAGACTATTTTACCTTTACAAGACATGAACCCATTGGAGTGTGTGGACAGATCATCCCATGGAA
CTTCCCCCTGCTGATGTTTGCCTGGAAAATAGCTCCAGCTTTGTGCTGTGGCAATACAGTAGTTATTAAG
CCAGCAGAGCAAACACCACTCAGTGCACTCTACATGGGAGCCCTCATCAAGGAGGCTGGCTTTCCTCCCG
GGGTCATCAATATTTTGCCAGGATATGGGCCAACGGCTGGGGCAGCAATAGCTTCTCACATTGGCATAGA
CAAGATTGCATTCACAGGGTCTACTGAGGTTGGAAAGCTTATCCAAGAAGCAGCTGGAAGAAGTAATTTG
AAGAGAGTAACTCTGGAACTTGGAGGCAAAAGTCCTAATATTATTTTTGCTGATGCTGACTTGGACTATG
CTGTGGAGCAGGCCCACCAGGGTGTGTTCTTCAATCAAGGTCAGTGCTGCACTGCAGGCTCTCGCATCTT
CGTGGAGGAGTCCATCTATGAGGAGTTTGTGAGAAGAAGCGTGGAGCGGGCCAAGAGGCGCGTAGTGGGG
AGTCCCTTTGACCCCACCACTGAGCAGGGTCCCCAGATTGATAAGAAACAGTACAACAAGATCTTGGAAC
TCATCCAGAGTGGTGTGGCTGAGGGCGCCAAGCTGGAATGTGGAGGCAAAGGACTGGGCCGAAAGGGGTT
TTTCATTGAGCCCACAGTGTTTTCCAACGTCACTGATGATATGCGGATTGCCAAGGAGGAGATCTTTGGC
CCTGTTCAGGAAATTTTGAGATTTAAGACGATGGATGAAGTTATCGAAAGAGCCAATAACTCAGACTTTG
GACTCGTAGCAGCTGTCTTTACTAATGACATCAACAAGGCCCTCACAGTGTCTTCTGCAATGCAAGCTGG
GACTGTTTGGATCAATTGTTACAATGCCTTAAATGCCCAGAGCCCCTTTGGGGGATTCAAGATGTCTGGA
AATGGGAGAGAAATGGGAGAATTTGGCTTGCGGGAGTACTCAGAAGTTAAGACGGTGACAGTAAAGATCC
CCCAGAAGAACTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223250 representing NM_003888
Red=Cloning site Green=Tags(s)

MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQ
EADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDL
QGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTVVIK
PAEQTPLSALYMGALIKEAGFPPGVINILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNL
KRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVG
SPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFG
PVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSG
NGREMGEFGLREYSEVKTVTVKIPQKNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003888
ORF Size 1554 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003888.4
RefSeq Size 3398 bp
RefSeq ORF 1557 bp
Locus ID 8854
UniProt ID O94788
Domains aldedh
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Retinol metabolism
MW 56.5 kDa
Gene Summary This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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