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FUT8 (NM_178155) Human Tagged ORF Clone
CAT＃: RC223075
FUT8 (Myc-DDK-tagged)-Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1
ORF Plasmid: tGFP
"NM_178155" in other vectors (6)
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CNY 4,280.00
CNY 4,560.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDGF; CDGF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223075 representing NM_178155
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACCTTGCTGTTTT ATATAGGTGGTCACTTGGTACGAGATAATGACCATCCTGATCACTCTAGCCGAGAACTGTCCAAGATTCT GGCAAAGCTTGAACGCTTAAAACAACAGAATGAAGACTTGAGGCGAATGGCCGAATCTCTCCGGATACCA GAAGGCCCTATTGATCAGGGGCCAGCTATAGGAAGAGTACGCGTTTTAGAAGAGCAGCTTGTTAAGGCCA AAGAACAGATTGAAAATTACAAGAAACAGACCAGAAATGGTCTGGGGAAGGATCATGAAATCCTGAGGAG GAGGATTGAAAATGGAGCTAAAGAGCTCTGGTTTTTCCTACAGAGTGAATTGAAGAAATTAAAGAACTTA GAAGGAAATGAACTCCAAAGACATGCAGATGAATTTCTTTTGGATTTAGGACATCATGAAAGGTCTATAA TGACGGATCTATACTACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGGGAAAAAGAGGCCAAAGATCT GACAGAACTGGTTCAGCGGAGAATAACATATCTTCAGAATCCCAAGGACTGCAGCAAAGCCAAAAAGCTG GTGTGTAATATCAACAAAGGCTGTGGCTATGGCTGTCAGCTCCATCATGTGGTCTACTGCTTCATGATTG CATATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGAC TGTATTTAGGCCTGTAAGTGAGACATGCACAGACAGATCTGGCATCTCCACTGGACACTGGTCAGGTGAA GTGAAGGACAAAAATGTTCAAGTGGTCGAGCTTCCCATTGTAGACAGTCTTCATCCCCGTCCTCCATATT TACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCATGGTGACCCTGCAGTGTGGTG GGTGTCTCAGTTTGTCAAATACTTGATCCGCCCACAGCCTTGGCTAGAAAAAGAAATAGAAGAAGCCACC AAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCATGTCAGACGCACAGACAAAGTGGGAACAGAAG CTGCCTTCCATCCCATTGAAGAGTACATGGTGCATGTTGAAGAACATTTTCAGCTTCTTGCACGCAGAAT GCAAGTGGACAAAAAAAGAGTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAG TACCCCAATTATGAATTTATTAGTGATAACTCTATTTCCTGGTCAGCTGGACTGCACAATCGATACACAG AAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCAGACTTCCTAGTGTGTACTTT TTCATCCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAAACACTACATCCTGATGCCTCTGCAAACTTC CATTCTTTAGATGACATCTACTATTTTGGGGGCCAGAATGCCCACAATCAAATTGCCATTTATGCTCACC AACCCCGAACTGCAGATGAAATTCCCATGGAACCTGGAGATATCATTGGTGTGGCTGGAAATCATTGGGA TGGCTATTCTAAAGGTGTCAACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGAGAG AAGATAGAAACGGTCAAGTACCCCACATATCCTGAGGCTGAGAAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223075 representing NM_178155
Red=Cloning site Green=Tags(s) MRPWTGSWRWIMLILFAWGTLLFYIGGHLVRDNDHPDHSSRELSKILAKLERLKQQNEDLRRMAESLRIP EGPIDQGPAIGRVRVLEEQLVKAKEQIENYKKQTRNGLGKDHEILRRRIENGAKELWFFLQSELKKLKNL EGNELQRHADEFLLDLGHHERSIMTDLYYLSQTDGAGDWREKEAKDLTELVQRRITYLQNPKDCSKAKKL VCNINKGCGYGCQLHHVVYCFMIAYGTQRTLILESQNWRYATGGWETVFRPVSETCTDRSGISTGHWSGE VKDKNVQVVELPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEAT KKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTK YPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANF HSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVRE KIETVKYPTYPEAEK SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178155 |
| ORF Size | 1725 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178155.3 |
| RefSeq Size | 3775 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 2530 |
| UniProt ID | Q9BYC5 |
| Protein Families | Transmembrane |
| Protein Pathways | Keratan sulfate biosynthesis, Metabolic pathways, N-Glycan biosynthesis |
| MW | 66.3 kDa |
| Gene Summary | This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Comprehensive Analysis of FUT8 Overexpressing Prostate Cancer Cells Reveals the Role of EGFR in Castration Resistance
,Höti, N;Lih, TS;Pan, J;Zhou, Y;Yang, G;Deng, A;Chen, L;Dong, M;Yang, RB;Tu, CF;Haffner, MC;Kay Li, Q;Zhang, H;,
Cancers (Basel) 2020
,PubMed ID 32085441
[FUT8]
|
|
Overexpression of alpha (1,6) fucosyltransferase associated with aggressive prostate cancer
,Wang, X;Chen, J;Li, QK;Peskoe, SB;Zhang, B;Choi, C;Platz, EA;Zhang, H;,
Glycobiology
,PubMed ID 24906821
[FUT8]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223075L1 | Lenti ORF clone of Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1, Myc-DDK-tagged |
CNY 6,680.00 |
|
| RC223075L2 | Lenti ORF clone of Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1, mGFP tagged |
CNY 6,680.00 |
|
| RC223075L3 | Lenti ORF clone of Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1, Myc-DDK-tagged |
CNY 6,680.00 |
|
| RC223075L4 | Lenti ORF clone of Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG223075 | FUT8 (tGFP-tagged) - Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1 |
CNY 5,880.00 |
|
| SC314071 | FUT8 (untagged)-Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 1 |
CNY 9,220.00 |
