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PGBD1 (NM_032507) Human Tagged ORF Clone
CAT＃: RC223042
PGBD1 (Myc-DDK-tagged)-Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2
ORF Plasmid: tGFP
"NM_032507" in other vectors (4)
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CNY 5,928.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ874C20.4; HUCEP-4; SCAND4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223042 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGAAGCTTTGCCAGGCCCTGCTCCTGAAAATGAAGATGGCCTTGTGAAAGTGAAGGAGGAAGATC CCACCTGGGAGCAGGTGTGCAACTCACAGGAGGGCAGCTCCCACACTCAGGAGATTTGCCGCCTGCGCTT TCGGCACTTCTGCTACCAGGAGGCTCACGGACCCCAGGAAGCTCTGGCCCAACTCCGAGAACTTTGTCAT CAATGGCTGAGACCGGAGATGCACACCAAGGAACAGATAATGGAACTGCTGGTGCTGGAGCAGTTCCTGA CCATCCTGCCCAAGGAGCTCCAGCCCTGTGTGAAGACATATCCTCTGGAGAGTGGAGAGGAGGCAGTGAC AGTGCTGGAGAATCTAGAGACAGGAAGTGGAGACACAGGACAACAGGCCTCTGTCTATATTCAGGGACAG GACATGCACCCAATGGTGGCAGAATATCAAGGAGTCTCTTTGGAGTGTCAGAGCCTCCAGCTCCTGCCTG GGATAACCACCCTGAAGTGTGAACCTCCACAGCGTCCTCAAGGGAACCCCCAAGAAGTGAGTGGGCCTGT TCCCCACGGATCAGCTCATCTCCAGGAAAAAAACCCCAGAGACAAGGCTGTAGTGCCTGTGTTTAACCCA GTCAGGTCCCAGACATTGGTGAAGACTGAGGAAGAAACAGCCCAGGCCGTTGCTGCAGAGAAGTGGTCAC ATCTGAGTCTGACTCGGAGGAACCTCTGTGGGAACTCAGCTCAGGAGACAGTTATGAGCCTCAGTCCGAT GACTGAAGAAATTGTAACTAAAGATAGATTGTTTAAAGCAAAGCAAGAAACTTCTGAAGAAATGGAACAA AGTGGAGAAGCCTCAGGAAAGCCCAACAGAGAGTGTGCACCCCAGATTCCTTGTAGTACTCCTATTGCTA CTGAAAGGACAGTTGCACATTTGAACACTCTGAAGGACCGTCACCCAGGTGATTTGTGGGCCCGCATGCA CATCTCATCCCTGGAATATGCTGCAGGAGACATTACCCGAAAAGGGAGAAAAAAAGACAAAGCTCGAGTG AGTGAACTGCTCCAAGGCCTCTCATTCTCTGGTGACTCAGATGTGGAAAAAGATAATGAGCCTGAGATCC AGCCTGCTCAAAAGAAGTTAAAGGTATCATGTTTCCCAGAAAAGAGTTGGACCAAAAGAGACATTAAACC CAATTTTCCAAGCTGGTCAGCACTGGATTCTGGACTTTTGAATCTCAAGAGCGAAAAGTTGAACCCAGTA GAGCTTTTTGAATTATTTTTTGATGATGAAACATTCAACTTAATTGTCAATGAAACCAATAATTATGCTT CTCAGAAAAATGTCAGCTTGGAAGTCACAGTTCAGGAAATGAGGTGTGTGTTTGGTGTCTTACTTTTGAG TGGATTTATGAGGCATCCTAGAAGGGAAATGTATTGGGAAGTCTCTGACACCGATCAGAACCTGGTTAGA GATGCAATCAGAAGGGACAGATTTGAATTGATTTTCTCAAACCTGCACTTTGCAGATAATGGCCACCTAG ATCAAAAAGATAAGTTTACAAAGTTGAGACCTCTCATAAAACAAATGAATAAAAATTTCCTCTTGTATGC TCCCCTGGAAGAATACTATTGCTTTGATAAGTCAATGTGTGAATGCTTTGATAGTGACCAATTCCTGAAT GGAAAGCCTATTAGAATTGGCTATAAAATTTGGTGTGGTACAACCACACAGGGTTATCTGGTTTGGTTTG AACCCTATCAAGAAGAATCAACTATGAAGGTAGATGAGGATCCTGATCTTGGGTTAGGTGGAAATCTAGT GATGAACTTCGCTGATGTTCTTTTAGAGAGAGGTCAGTATCCCTATCACCTGTGTTTTGATAGCTTCTTT ACAAGTGTCAAATTGTTGTCAGCCTTGAAAAAGAAGGGGGTGAGGGCAACAGGAACAATTCGTGAGAACA GGACCGAAAAATGTCCCCTTATGAATGTAGAACATATGAAAAAAATGAAGAGAGGGTATTTTGATTTCCG AATAGAAGAAAACAATGAGATAATTTTGTGTCGTTGGTATGGGGATGGCATTATCAGTCTGTGCTCCAAT GCTGTGGGCATAGAACCAGTCAATGAGGTAAGCTGTTGTGATGCTGATAACGAAGAAATCCCTCAGATAA GTCAACCATCCATAGTAAAAGTGTATGATGAATGCAAGGAAGGTGTAGCTAAAATGGATCAAATTATTTC GAAATACAGGGTGAGGATAAGAAGCAAGAAATGGTACTCAATTTTGGTGAGCTACATGATTGATGTAGCC ATGAACAATGCATGGCAACTACACAGAGCCTGTAACCCAGGTGCTTCTCTAGACCCCTTGGATTTTCGGA GATTTGTTGCACATTTCTACTTGGAACACAATGCTCATCTGTCAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223042 protein sequence
Red=Cloning site Green=Tags(s) MYEALPGPAPENEDGLVKVKEEDPTWEQVCNSQEGSSHTQEICRLRFRHFCYQEAHGPQEALAQLRELCH QWLRPEMHTKEQIMELLVLEQFLTILPKELQPCVKTYPLESGEEAVTVLENLETGSGDTGQQASVYIQGQ DMHPMVAEYQGVSLECQSLQLLPGITTLKCEPPQRPQGNPQEVSGPVPHGSAHLQEKNPRDKAVVPVFNP VRSQTLVKTEEETAQAVAAEKWSHLSLTRRNLCGNSAQETVMSLSPMTEEIVTKDRLFKAKQETSEEMEQ SGEASGKPNRECAPQIPCSTPIATERTVAHLNTLKDRHPGDLWARMHISSLEYAAGDITRKGRKKDKARV SELLQGLSFSGDSDVEKDNEPEIQPAQKKLKVSCFPEKSWTKRDIKPNFPSWSALDSGLLNLKSEKLNPV ELFELFFDDETFNLIVNETNNYASQKNVSLEVTVQEMRCVFGVLLLSGFMRHPRREMYWEVSDTDQNLVR DAIRRDRFELIFSNLHFADNGHLDQKDKFTKLRPLIKQMNKNFLLYAPLEEYYCFDKSMCECFDSDQFLN GKPIRIGYKIWCGTTTQGYLVWFEPYQEESTMKVDEDPDLGLGGNLVMNFADVLLERGQYPYHLCFDSFF TSVKLLSALKKKGVRATGTIRENRTEKCPLMNVEHMKKMKRGYFDFRIEENNEIILCRWYGDGIISLCSN AVGIEPVNEVSCCDADNEEIPQISQPSIVKVYDECKEGVAKMDQIISKYRVRIRSKKWYSILVSYMIDVA MNNAWQLHRACNPGASLDPLDFRRFVAHFYLEHNAHLSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032507 |
| ORF Size | 2427 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032507.3, NP_115896.1 |
| RefSeq Size | 3135 bp |
| RefSeq ORF | 2430 bp |
| Locus ID | 84547 |
| UniProt ID | Q96JS3 |
| Domains | LER |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 92.5 kDa |
| Gene Summary | The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. This gene product is specifically expressed in the brain, however, its exact function is not known. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223042L3 | Lenti ORF clone of Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC223042L4 | Lenti ORF clone of Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG223042 | PGBD1 (tGFP-tagged) - Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2 |
CNY 7,528.00 |
|
| SC314746 | PGBD1 (untagged)-Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2 |
CNY 7,220.00 |
