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FREM1 (NM_144966) Human Tagged ORF Clone
CAT＃: RC222886
FREM1 (Myc-DDK-tagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1
ORF Plasmid: tGFP
"NM_144966" in other vectors (4)
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CNY 14,384.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BNAR; C9orf143; C9orf145; C9orf154; MOTA; TILRR; TRIGNO2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222886 representing NM_144966
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCTCTGAGTTGGGGGGCTGCGAATGCCGTGCTGCTGCTGCTCCTCCTGGCCTGGGCCAGCCCCA CCTTCATCAGCATCAACCGCGGGGTGAGGGTGATGAAGGGCCACTCTGCCTTCCTGTCAGGAGATGACCT GAAGTTTGCCATCCCTAAAGAGAAAGATGCCTGCAAAGTGGAAGTTGTGATGAATGAGCCAATAACCCAG AGGGTTGGGAAACTCACTCCACAGGTCTTTGACTGCCATTTCCTTCCCAACGAAGTCAAGTATGTTCACA ATGGTTGTCCAATTCTTGATGAAGACACAGTGAAGCTCAGACTTTACAGATTTACTGAAAGAGATACCTT CATAGAAACTTTTATCCTGTGGGTCTATCTCCTGGAACCAGACTGTAACATCATCCATATGAGTAACAAT GTGCTGGAGGTGCCTGAATTCAATGGCTTGTCCCAAGCGATTGATAAAAATCTGCTCAGATTCGATTATG ATAGGATGGCTAGCCTGGAATGTACCGTCAGCCTGGACACTGCGAGAACTCGGCTGCCAGCCCATGGCCA GATGGTTCTCGGGGAGCCTCGACCAGAAGAACCTCGTGGAGATCAGCCACACAGTTTTTTCCCAGAGTCC CAACTGAGAGCAAAACTGAAGTGTCCAGGTGGGAGCTGTACCCCAGGATTAAAGAAAATAGGAAGCCTCA AAGTGAGCTGTGAGGAGTTCCTGCTGATGGGCCTTCGTTATCAGCATCTGGATCCCCCTTCACCCAACAT TGATTATATCTCCATCCAACTGGACCTCACAGATACCAGGAGCAAAATTGTGTACAAGTCAGAGAGTGCG TGGCTGCCTGTCTATATCAGAGCTGGAATTCCGAATCAGATTCCAAAGGCTGCATTCATGGCCGTGTTTA TTCTGGAAGTGGATCAGTTCATCCTGACCTCCTTGACTACATCAGTTCTGGACTGTGAAGAAGATGAGAC CCCTAAACCCTTGCTGGTGTTCAACATTACTAAAGCCCCGCTCCAGGGCTATGTGACTCACCTGTTGGAT CACACCAGACCAATCTCCTCATTCACCTGGAAAGATCTCAGTGACATGCAGATCGCCTATCAGCCACCAA ACAGCAGCCATTCTGAGAGGAGACATGATGAGGTAGAATTGGAGGTATACGACTTCTTCTTTGAAAGGAG TGCACCTATGACAGTCCACATCTCCATCAGAACAGCAGATACAAATGCCCCCCGTGTATCCTGGAATACA GGTCTGAGTCTCCTTGAGGGGCAGTCTCGAGCCATCACTTGGGAACAGTTTCAGGTTGTCGACAATGACG ACATTGGTGCTGTCCGGCTAGTCACCGTTGGTGGCCTGCAGCATGGATGGCTGACTTTAAGAGGGGGGAA AGGGTTTCTCTTCACCGTGGCTGACCTCCAGGCTGGAGTTGTTCGCTATCATCATGATGACAGCGACTCC ACCAAAGACTTCGTGGTCTTCCGGATATTTGATGGCCATCACAGCATCCGTCACAAATTCCCCATCAACG TCTTGCCCAAAGATGATAGTCCCCCGTTCCTCATAACCAATGTTGTGATTGAACTGGAGGAGGGGCAGAC CATCCTGATCCAGGGATCCATGCTGCGAGCTTCAGATGTGGACGCCAGTGATGACTACATCTTCTTCAAT ATCACAAAGCCTCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAGGACTGATAGGCTATCCTGTCC ATGGCTTCCTTCAGAGGGATTTGTTTAATGGAATCATTTATTATCGTCATTTTGGTGGAGAAATCTTTGA AGATTCTTTTCAATTTGTCCTGTGGGACAGCCATGAACCTCCAAATCTTTCAGTGCCACAGGTGGCAACA ATCCATATAACTCCAGTGGATGACCAGCTTCCAAAAGAGGCTCCTGGAGTTTCTCGGCATTTGGTTGTCA AGGAAACTGAGGTGGCCTATATAACTAAGAAACAGCTACATTTTATAGATTCAGAATCATATGACAGGGA GCTGGTCTACACAATAACTACTCCTCCATTTTTCTCCTTCAGCCACAGACACTTGGATGCTGGGAAATTA TTTATGGTGGACAGCATACCAAAAGTAGTTAAGAATCCTACGGCCCTGGAGCTGAGGTCATTCACTCAGC ATGCTGTGAACTATATGAAAGTGGCCTACATGCCCCCCATGCAAGACATTGGTCCCCATTGCAGAGATGT CCAGTTCACATTTTCTGTCAGTAACCAACATGGCGGTACTTTGCATGGGATCTGCTTTAACATTACAATC CTCCCAGTGGACAATCAAGTTCCTGAGGCGTTCACCAACCCTCTGAAAGTGACTGAGGGAGGTCAAAGCA TCATCAGCACAGAGCACATTCTAATTTCTGATGCAGATACCAAGCTGGACAATATTGACCTCTCCCTGCG GGAATTGCCTCTGCACGGAAGGGTGGAGCTGAATGGATTTCCTCTAAATTCAGGGGGCACATTTTCTTGG GGCGATCTCCATACCTTAAAAGTTAGGTATCAACATGATGGAACTGAAGTTCTTCAGGATGACCTACTCT TGGAGGTCACCGATGGCACAAATTCAGCAGAATTTGTACTACATGTTGAGGTATTCCCTGTCAACGATGA GCCACCAGTCTTAAAGGCTGACCTCATGCCTGTCATGAATTGCTCAGAGGGAGGAGAGGTGGTCATCACC TCTGAATACATTTTTGCTACTGATGTGGACAGCGATAACTTGAAGTTGATGTTTGTGATTGCTCGCGAAC CTCAGCATGGGGTGGTGAGGAGAGCTGGAGTCACAGTGGATCAGTTCTCTCAGAGAGATGTTATCTCAGA GGCCGTGACATACAAACACACAGGTGGCGAGATTGGCCTGATGCCTTGTTTTGACACCATTACATTGGTG GTTTCGGATGGAGAGGCTGGCCCTTTTGTGAATGGCTGTTGCTACAATGGACCCAATCCATCTGTTCCAC TTCATGCGTCCTTTCCAGTGTATGATCTCAACATCACGGTATACCCAGTAGACAACCAGCCACCTTCCAT TGCAATAGGTCCCGTGTTTGTTGTAGATGAGGGCTGCTCAACAGCCCTTACTGTTAACCATTTGTCCGCC ACTGACCCTGACACTGCAGCAGATGACTTGGAATTTGTTTTGGTTTCTCCTCCTCAGTTTGGCTACCTCG AAAATATACTCCCTTCTGTGGGTTTTGAAAAAAGCAATATTGGCATAAGTATAGATTCATTTCAGTGGAA AGACATGAACGCTTTTCACATTAACTATGTGCAGTCCAGGCATCTGAGGATAGAACCAACTGCCGACCAG TTCACGGTGTACGTCACAGATGGGAAGCATCACTCCTTGGAGATACCATTTTCTATTATAATCAACCCCA CAAATGATGAAGCTCCTGACTTTGTAGTGCAGAATATTACTGTGTGTGAGGGTCAGATGAAAGAGCTGGA CTCTTCCATCATCAGCGCTGTGGACCTGGACATTCCCCAGGATGCCCTGCTGTTCAGCATCACTCAAAAG CCACGCCATGGCCTCCTCATCGATAGGGGGTTTAGCAAAGACTTCTCTGAGAATAAGCAGCCAGCCAACC CTCACCAGAAACATGCACCTGTTCACAGCTTTTCCATGGAACTCCTCAAGACTGGAATGAGGTTGACGTA CATGCATGATGACTCAGAGAGCCTTGCTGATGATTTTACAATCCAATTGTCAGATGGGAAACATAAGATA CTTAAAACCATTTCAGTAGAGGTCATCCCAGTTAATGATGAAAAACCAATGCTGAGCAAGAAGGCTGAAA TTGCAATGAATATGGGTGAAACTCGTATTATTTCCAGTGCTATTCTTTCAGCCATAGATGAAGACTCACC CAGGGAGAAGATTTACTATGTATTTGAAAGGCTTCCCCAAAATGGGCAACTTCAGCTTAAGATAGGGAGG GACTGGGTTCCTCTCTCCCCTGGCATGAAATGCACTCAGGAGGAAGTGGATCTGAACTTGCTGAGATACA CACACACCGGGGCAATGGATTCCCAGAATCAAGATAGCTTCACCTTCTACCTTTGGGATGGCAACAACAG GTCCCCTGCTCTTGACTGTCAAATCACCATCAAGGACATGGAAAAAGGTGATATTGTCATCCTTACAAAA CCACTCGTGGTGTCTAAAGGTGATAGAGGTTTCTTAACAACCACCACGCTCCTGGCTGTGGACGGAACAG ACAAGCCTGAGGAACTGCTCTATGTCATCACCTCCCCTCCGCGATATGGCCAGATCGAATATGTTCACTA TCCTGGAGTTCCCATTACAAACTTCAGCCAAATGGATGTAGTGGGGCAGACAGTTTGCTATGTACACAAG AGCAAGGTGACTGTCTCCAGTGACAGATTCAGATTCATCATCAGCAATGGACTGCGGACCGAGCACGGGG TGTTTGAGATCACACTGGAGACTGTGGACAGAGCCCTGCCTGTGGTAACCAGGAACAAGGGGTTGAGACT GGCCCAAGGGGCCGTGGGCCTGCTTTCCCCTGACCTCCTTCAGCTGACCGACCCTGATACACCTGCGGAG AACCTCACCTTCCTCTTGGTTCAGCTCCCCCAGCATGGCCAGCTCTACCTGTGGGGGACAGGGCTACTTC AACACAATTTCACCCAGCAGGATGTGGACAGCAAGAATGTGGCCTATCGGCACTCAGGAGGGGACTCCCA GACTGACTGCTTTACTTTCATGGCCACAGATGGGACAAACCAAGGCTTTATTGTGAATGGGAGAGTGTGG GAAGAACCTGTTTTATTCACCATTCAGGTAGACCAATTGGACAAAACAGCTCCTCGTATCACACTCTTGC ATTCCCCTTCTCAAGTGGGGCTCCTGAAAAATGGCTGCTACGGGATTTACATCACTTCCCGCGTGTTGAA GGCATCAGACCCTGACACTGAGGACGATCAGATCATCTTTAAAATTCTACAAGGCCCAAAACATGGACAT CTGGAGAACACAACAACAGGTGAATTTATCCATGAGAAATTTAGCCAAAAGGACTTAAACAGTAAGACTA TTCTTTACATCATAAACCCATCTTTGGAAGTAAATTCAGATACCGTGGAATTTCAAATCATGGACCCCAC AGGGAACTCGGCCACTCCTCAAATTTTGGAACTGAAGTGGTCTCATATTGAATGGTCACAGACCGAATAT GAAGTCTGTGAGAATGTGGGTTTGTTGCCCTTGGAAATTATCAGAAGGGGATATTCCATGGACTCGGCCT TTGTGGGTATAAAGGTCAACCAAGTGTCAGCTGCAGTTGGAAAAGATTTCACCGTGATTCCATCTAAACT GATTCAGTTTGACCCAGGAATGTCAACTAAGATGTGGAATATAGCAATTACCTATGACGGATTAGAGGAA GATGATGAGGTCTTTGAAGTAATTCTGAACTCCCCTGTGAATGCAGTTCTTGGCACAAAGACAAAAGCTG CAGTGAAAATTTTGGACTCAAAAGGAGGACAATGCCATCCTTCATATTCCTCCAACCAAAGCAAGCACAG CACATGGGAGAAGGGCATTTGGCATCTGCTGCCCCCAGGGTCTTCCTCATCCACCACTTCTGGTTCCTTT CATCTGGAAAGAAGACCTCTTCCATCTTCCATGCAGCTAGCAGTCATCAGGGGAGACACCCTGCGGGGCT TTGATTCTACAGATCTTTCTCAAAGGAAGCTTAGGACCCGTGGGAATGGCAAAACAGTTCGTCCATCCTC TGTTTATAGAAATGGAACAGACATCATCTATAATTATCATGGGATAGTTTCCTTGAAACTGGAGGATGAC AGTTTCCCAACTCACAAAAGGAAGGCCAAAGTATCCATCATTAGTCAGCCACAAAAGACAATCAAAGTGG CAGAACTGCCTCAAGCAGATAAGGTGGAATCCACAACTGACTCACACTTCCCCAGACAGGACCAGTTGCC CTCATTTCCAAAGAACTGCACTCTGGAATTAAAGGGACTCTTCCATTTTGAAGAAGGCATCCAGAAGCTG TATCAGTGCAATGGGATCGCCTGGAAAGCCTGGAGTCCCCAAACCAAGGATGTGGAAGACAAATCCTGTC CAGCCGGGTGGCACCAGCACTCAGGCTACTGTCACATCTTGATCACAGAGCAGAAAGGCACCTGGAATGC GGCTGCCCAAGCTTGCAGGGAACAATACCTGGGCAACCTTGTAACTGTATTCTCCAGGCAGCACATGCGG TGGCTCTGGGACATTGGTGGGAGAAAGTCCTTTTGGATAGGTTTGAACGACCAAGTGCATGCTGGCCACT GGGAGTGGATCGGTGGTGAACCTGTTGCCTTCACCAATGGGAGAAGAGGGCCCTCTCAACGCTCCAAGCT TGGAAAGAGCTGTGTTTTGGTTCAAAGACAAGGGAAATGGCAAACAAAAGACTGTAGGAGAGCCAAACCT CATAATTATGTGTGTTCCAGAAAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222886 representing NM_144966
Red=Cloning site Green=Tags(s) MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQ RVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNN VLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPES QLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESA WLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLD HTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNT GLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDS TKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFN ITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVAT IHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKL FMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITI LPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSW GDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVIT SEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLV VSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSA TDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQ FTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQK PRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKI LKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGR DWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTK PLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHK SKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAE NLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVW EEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGH LENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEY EVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEE DDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSF HLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDD SFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKL YQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMR WLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKP HNYVCSRKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144966 |
| ORF Size | 6537 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144966.7 |
| RefSeq Size | 7387 bp |
| RefSeq ORF | 6540 bp |
| Locus ID | 158326 |
| UniProt ID | Q5H8C1 |
| Protein Families | Protease, Transmembrane |
| MW | 241.9 kDa |
| Gene Summary | This gene encodes a basement membrane protein that may play a role in craniofacial and renal development. Mutations in this gene have been associated with bifid nose with or without anorectal and renal anomalies. Alternatively spliced transcript variants encoding different isoforms have been described. PubMed ID 19940113 describes one such variant that initiates transcription within a distinct, internal exon; the resulting shorter isoform (named Toll-like/interleukin-1 receptor regulator, TILRR) is suggested to be a co-receptor of the interleukin 1 receptor family and may regulate receptor function and Toll-like receptor/interleukin 1 receptor signal transduction, contributing to the control of inflammatory response activation. [provided by RefSeq, Apr 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222886L1 | Lenti ORF clone of Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1, Myc-DDK-tagged |
CNY 16,784.00 |
|
| RG222886 | FREM1 (tGFP-tagged) - Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1 |
CNY 15,984.00 |
|
| SC306199 | FREM1 (untagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1 |
CNY 41,990.00 |
|
| SC318714 | FREM1 (untagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1 |
CNY 41,990.00 |
