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Ancient ubiquitous protein 1 (AUP1) (NM_181575) Human Tagged ORF Clone
CAT＃: RC222822
- TrueORF®
AUP1 (Myc-DDK-tagged)-Human ancient ubiquitous protein 1 (AUP1)
ORF Plasmid: tGFP
"NM_181575" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222822 representing NM_181575
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTCCCTCAGGGCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCTTCCGGGTGACTGCTTCC TACTGCTCGTGCTGCTGCTCTACGCGCCAGTCGGGTTCTGCCTCCTCGTCCTGCGCCTCTTTCTCGGGAT CCACGTCTTCCTGGTCAGCTGCGCGCTGCCAGACAGCGTCCTTCGCAGATTCGTAGTGCGGACCATGTGT GCGGTGCTAGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGGGATCACAGTGTCAGGGTCCTCATTT CCAACCATGTGACACCTTTCGACCACAACATAGTCAATTTGCTTACCACCTGTAGCACCCCTCTACTCAA TAGTCCCCCCAGCTTTGTGTGCTGGTCTCGGGGCTTCATGGAGATGAATGGGCGGGGGGAGTTGGTGGAG TCACTCAAGAGATTCTGTGCTTCCACGAGGCTTCCCCCCACTCCTCTGCTGCTATTCCCTGAGGAAGAGG CCACCAATGGCCGGGAGGGGCTCCTGCGCTTCAGTTCCTGGCCATTTTCTATCCAAGATGTGGTACAACC TCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCTCCTGGGTCTCAGAACTG CTGTGGTCACTTTTCGTCCCTTTCACGGTGTATCAAGTAAGGTGGCTTCGTCCTGTTCATCGCCAACTAG GGGAAGCGAATGAGGAGTTTGCACTCCGTGTACAACAGCTGGTGGCCAAGGAATTGGGCCAGACAGGGAC ACGGCTCACTCCAGCTGACAAAGCAGAGCACATGAAGCGACAAAGACACCCCAGATTGCGCCCCCAGTCA GCCCAGTCTTCTTTCCCTCCCTCCCCTGGTCCTTCTCCTGATGTGCAACTGGCAACTCTGGCTCAGAGAG TCAAGGAAGTTTTGCCCCATGTGCCATTGGGTGTCATCCAGAGAGACCTGGCCAAGACTGGCTGTGTAGA CTTGACTATCACTAATCTGCTTGAGGGGGCCGTAGCTTTCATGCCTGAAGACATCACCAAGGGAACTCAG TCCCTACCCACAGCCTCTGCCTCCAAGTTTCCCAGCTCTGGCCCGGTGACCCCTCAGCCAACAGCCCTAA CATTTGCCAAGTCTTCCTGGGCCCGGCAGGAGAGCCTGCAGGAGCGCAAGCAAGCACTATATGAATACGC AAGAAGGAGATTCACAGAGAGACGAGCCCAGGAGGCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222822 representing NM_181575
Red=Cloning site Green=Tags(s) MELPSGPGPERLFDSHRLPGDCFLLLVLLLYAPVGFCLLVLRLFLGIHVFLVSCALPDSVLRRFVVRTMC AVLGLVARQEDSGLRDHSVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMNGRGELVE SLKRFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSEL LWSLFVPFTVYQVRWLRPVHRQLGEANEEFALRVQQLVAKELGQTGTRLTPADKAEHMKRQRHPRLRPQS AQSSFPPSPGPSPDVQLATLAQRVKEVLPHVPLGVIQRDLAKTGCVDLTITNLLEGAVAFMPEDITKGTQ SLPTASASKFPSSGPVTPQPTALTFAKSSWARQESLQERKQALYEYARRRFTERRAQEAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181575 |
| ORF Size | 1230 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181575.5 |
| RefSeq Size | 1561 bp |
| RefSeq ORF | 1233 bp |
| Locus ID | 550 |
| UniProt ID | Q9Y679 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 45.6 kDa |
| Gene Summary | The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222822L1 | Lenti ORF clone of Human ancient ubiquitous protein 1 (AUP1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222822L2 | Lenti ORF clone of Human ancient ubiquitous protein 1 (AUP1), mGFP tagged |
CNY 5,890.00 |
|
| RC222822L3 | Lenti ORF clone of Human ancient ubiquitous protein 1 (AUP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222822L4 | Lenti ORF clone of Human ancient ubiquitous protein 1 (AUP1), mGFP tagged |
CNY 5,890.00 |
|
| RG222822 | AUP1 (tGFP-tagged) - Human ancient ubiquitous protein 1 (AUP1) |
CNY 4,370.00 |
|
| SC310429 | AUP1 (untagged)-Human ancient ubiquitous protein 1 (AUP1) |
CNY 6,650.00 |
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