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RGNEF (ARHGEF28) (NM_001080479) Human Tagged ORF Clone
CAT＃: RC222773
- TrueORF®
ARHGEF28 (Myc-DDK-tagged)-Human 190 kDa guanine nucleotide exchange factor (RGNEF), transcript variant 1
ORF Plasmid: tGFP
"NM_001080479" in other vectors (4)
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CNY 11,296.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p190RHOGEF; RGNEF; RIP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222773 representing NM_001080479
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGAGCTGCAGCGAAGCACCTCTTTACGGGCAGATGATGATCTATGCGAAGTTTGACAAAAATG TGTATCTTCCTGAAGATGCTGAGTTTTACTTTACTTATGACGGATCTCATCAGCGACATGTCATGATTGC AGAGCGCATCGAGGATAACGTTCTCCAGTCCAGCGTCCCAGGCCATGGGCTTCAGGAGACGGTGACGGTA TCTGTGTGCCTCTGCTCGGAAGGTTACTCTCCGGTGACCATGGGCTCTGGCTCAGTGACCTACGTGGACA ACATGGCTTGCAGGCTGGCTCGTCTGCTGGTGACGCAGGCCAATCGCCTCACAGCCTGCAGCCACCAGAC CCTGCTGACCCCATTTGCCTTGACGGCAGGAGCACTGCCTGCCTTGGATGAGGAGCTCGTGCTGGCTCTG ACCCATCTGGAATTGCCTCTAGAGTGGACTGTGTTGGGAAGTTCTTCACTTGAAGTATCTTCTCACAGAG AATCTCTTCTACACCTGGCTATGAGATGGGGCCTGGCTAAACTTTCCCAGTTCTTCTTGTGTCTCCCGGG GGGAGTCCAGGCCTTGGCTTTACCCAACGAAGAGGGTGCCACACCATTAGACTTAGCTTTACGTGAAGGA CACTCCAAGCTGGTGGAAGACGTCACAAATTTTCAGGGCAGATGGTCCCCAAGCTTCTCCCGAGTGCAGC TCAGTGAAGAAGCCTCCTTGCATTACATTCACTCATCGGAAACGCTGACCCTGACCCTGAACCACACAGC CGAGCATTTGTTGGAGGCAGATATTAAACTCTTCCGGAAATACTTTTGGGATAGAGCCTTTCTTGTCAAG GCCTTTGAGCCAGAAGCCAGGCCAGAGGAAAGAACAGCTATGCCCTCCAGCGGTGCAGAAACTGAAGAAG AGATTAAGAATTCAGTGTCCAGCAGATCAGCAGCTGAAAAGGAAGATATAAAGCGTGTCAAAAGCCTGGT GGTTCAACACAATGAACATGAAGACCAGCACAGCCTAGATTTGGATCGCTCCTTCGATATCCTAAAAAAA TCCAAGCCGCCCTCGACATTGCTTGCTGCAGGCCGGCTTTCAGACATGCTGAATGGAGGTGATGAAGTCT ACGCTAACTGTATGGTGATTGATCAGGTTGGTGATTTGGATATCAGCTATATTAATATAGAGGGAATCAC TGCCACTACCAGCCCTGAATCCAGAGGTTGCACTCTGTGGCCTCAGAGCAGCAAACACACCCTTCCTACA GAAACCAGTCCCAGTGTGTACCCACTTAGTGAAAATGTCGAAGGGACAGCACACACTGAAGCCCAGCAGT CCTTCATGTCACCATCAAGTTCGTGTGCTTCCAACTTGAATCTTTCTTTTGGTTGGCATGGATTTGAAAA GGAACAAAGTCATCTAAAGAAAAGAAGTTCTAGCCTTGATGCCTTGGACGCCGACAGTGAAGGGGAAGGG CATTCTGAGCCATCCCACATCTGTTACACTCCAGGGTCTCAGAGCTCCTCAAGAACTGGGATTCCTAGTG GGGATGAATTGGACTCTTTTGAGACTAACACTGAACCGGATTTTAATATCTCCAGGGCTGAATCCCTTCC TCTATCAAGTAATCTACAGTCGAAGGAATCACTGCTTTCTGGAGTTCGCTCACGTTCTTATTCTTGCTCA TCACCCAAAATTTCTTTAGGAAAAACTCGTTTGGTGCGTGAATTAACAGTATGCAGTTCAAGTGAAGAGC AAAGAGCTTACAGCTTATCGGAGCCACCAAGAGAAAACAGAATTCAGGAAGAAGAATGGGATAAATACAT CATACCTGCCAAATCAGAGTCTGAAAAATATAAAGTGAGTCGAACTTTCAGTTTCCTCATGAATAGGATG ACTAGCCCTCGGAATAAATCAAAGACAAAAAGCAAGGATGCCAAAGATAAAGAGAAGCTGAATCGACATC AGTTTGCCCCAGGAACATTCTCTGGGGTTCTGCAGTGTTTGGTTTGTGATAAAACACTCCTGGGGAAAGA GTCACTGCAGTGTTCTAACTGTAATGCAAATGTGCACAAAGGTTGTAAAGATGCTGCGCCTGCATGCACC AAGAAATTCCAAGAGAAATATAACAAGAACAAACCACAGACCATCCTTGGAAATTCTTCATTTAGAGACA TCCCACAGCCTGGTCTCTCCTTGCACCCTTCTTCCTCCGTGCCTGTTGGATTGCCGACTGGAAGGAGGGA GACTGTGGGACAGGTCCATCCATTGTCCAGAAGTGTTCCAGGCACCACCTTGGAAAGCTTCAGGAGGTCA GCCACATCCTTGGAGTCTGAGAGTGACCATAACAGCTGCAGAAGCAGGTCTCATTCTGATGAGCTGCTAC AGTCCATGGGCTCTTCTCCCTCTACAGAGTCTTTCATAATGGAAGATGTTGTGGATTCTTCTCTGTGGAG TGACCTCAGCAGTGATGCCCAGGAGTTTGAAGCAGAATCTTGGAGTCTTGTGGTGGATCCCTCATTTTGT AATAGGCAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTAATGCAAACAGAGATGCATC ACATCCAGACCCTGTTCATCATGTCTGAGATCTTCAGGAAAGGCATGAAAGAGGAGCTGCAGCTGGACCA CAGCACCGTGGATAAAATTTTCCCCTGTTTAGATGAGTTGCTTGAAATCCACAGGCATTTCTTCTACAGT ATGAAGGAACGAAGGCAGGAATCCTGTGCTGGCAGCGACAGGAATTTTGTGATCGACCGAATTGGAGATA TTTTGGTACAACAGTTTTCAGAAGAAAATGCAAGTAAAATGAAGAAAATATATGGAGAATTCTGTTGCCA TCATAAAGAAGCTGTTAACCTCTTTAAAGAACTCCAGCAGAATAAAAAGTTTCAGAATTTTATTAAGCTC CGAAATAGTAATCTTTTGGCTCGACGCCGAGGAATTCCAGAATGCATTCTGTTGGTCACTCAGCGTATTA CAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACACAAAGGAAAGAACTGAGGAACATAAAGACTT ACGCAAAGCGCTTTGCTTAATTAAAGACATGATTGCAACAGTGGATTTAAAAGTCAATGAATATGAGAAA AACCAAAAATGGCTTGAGATCCTAAATAAGATTGAAAACAAAACATACACGAAGCTCAAAAATGGACATG TGTTTAGGAAGCAGGCACTGATGAGTGAAGAAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAAC TGCTACAGGTCGTTTCAAAGATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTTTACAAGAAAAA GACCAGAAATACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTTCCCTTCAAAAGCTTATTGCTA GAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCAGTGCTTCATCTGCTGGTCCTGAGATGTATGA AATTCACACCAATTCCAAGGAGGAACGCAATAACTGGATGAGACGGATCCAGCAGGCTGTAGAAAGTTGT CCTGAAGAAAAAGGGGGAAGGACAAGTGAATCTGATGAAGACAAGAGGAAAGCTGAAGCCAGAGTGGCCA AAATTCAGCAATGTCAAGAAATACTCACTAACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGCT GCATATCTATGCTGAACTTGGAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCCCACCTCCTTATT AAACCTGACCCAGGCGAGCCTCCCCAGGCAGCCTCATTACTGGCAGCAGCACTGAAAGAAGCTGAGAGCC TACAAGTTGCAGTGAAGGCCTCACAGATGGGCGCCGTGAGTCAATCATGTGAGGACAGTTGTGGAGACTC TGTCTTGGCGGACACACTCAGTTCTCATGATGTACCAGGATCACCGACTGCCTCATTAGTCACAGGAGGG AGAGAAGGAAGAGGCTGTTCGGATGTGGATCCCGGGATCCAGGGTGTGGTAACCGACTTGGCCGTCTCTG ATGCAGGGGAGAAGGTGGAATGTAGAAATTTTCCAGGTTCTTCACAATCAGAGATTATACAAGCCATACA GAATTTAACCCGTCTCTTATACAGCCTTCAGGCCGCCTTGACCATTCAGGACAGCCACATTGAGATCCAC AGGCTGGTTCTCCAGCAGCAGGAGGGCCTGTCTCTCGGCCACTCTATCCTCCGAGGCGGCCCCTTGCAGG ACCAGAAGTCTCGCGACGCGGACAGGCAGCATGAGGAGCTGGCCAATGTGCACCAGCTTCAGCACCAGCT CCAGCAGGAGCAGCGGCGCTGGCTGCGCAGGTGTGAGCAGCAGCAGCGGGCGCAGGCGACCAGGGAGAGC TGGCTGCAGGAGCGGGAGCGGGAGTGCCAGTCGCAGGAGGAGCTGCTGCTGCGGAGCCGGGGCGAGCTGG ACCTCCAGCTCCAGGAGTACCAGCACAGCCTGGAGCGGCTGAGGGAGGGCCAGCGCCTGGTGGAGAGGGA GCAGGCGAGGATGCGGGCCCAGCAGAGCCTGCTGGGCCACTGGAAGCACGGCCGGCAGAGGAGCCTGCCC GCGGTGCTCCTTCCGGGTGGCCCCGAGGTAATGGAACTTAATCGATCTGAGAGTTTATGTCATGAAAACT CATTCTTCATCAATGAAGCTTTAGTACAAATGTCATTTAACACTTTCAACAAACTGAATCCATCAGTTAT CCATCAGGATGCCACTTACCCTACAACTCAATCTCATTCTGACTTGGTGAGGACTAGTGAACATCAAGTA GACCTCAAGGTGGACCCTTCTCAGCCTTCGAATGTCAGTCACAAACTGTGGACAGCCGCTGGTTCCGGCC ATCAGATACTTCCTTTCCATGAAAGCAGCAAGGATTCTTGTAAAAATGGTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222773 representing NM_001080479
Red=Cloning site Green=Tags(s) MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTV SVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLAL THLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREG HSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVK AFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKK SKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPT ETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEG HSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCS SPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRM TSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACT KKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRS ATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFC NRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYS MKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKL RNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEK NQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEK DQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESC PEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLI KPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGG REGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIH RLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRES WLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLP AVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQV DLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080479 |
| ORF Size | 4953 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080479.1, NP_001073948.1 |
| RefSeq Size | 5252 bp |
| RefSeq ORF | 5196 bp |
| Locus ID | 64283 |
| UniProt ID | Q8N1W1 |
| MW | 185.7 kDa |
| Gene Summary | This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222773L3 | Lenti-ORF clone of ARHGEF28 (Myc-DDK-tagged)-Human 190 kDa guanine nucleotide exchange factor (RGNEF), transcript variant 1 |
CNY 18,340.00 |
|
| RC222773L4 | Lenti-ORF clone of ARHGEF28 (mGFP-tagged)-Human 190 kDa guanine nucleotide exchange factor (RGNEF), transcript variant 1 |
CNY 18,340.00 |
|
| RG222773 | ARHGEF28 (tGFP-tagged) - Human 190 kDa guanine nucleotide exchange factor (RGNEF), transcript variant 1 |
CNY 18,050.00 |
|
| SC315889 | ARHGEF28 (untagged)-Human 190 kDa guanine nucleotide exchange factor (RGNEF), transcript variant 1 |
CNY 26,790.00 |
