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Glucose Transporter GLUT1 (SLC2A1) (NM_006516) Human Tagged ORF Clone
CAT＃: RC222696
SLC2A1 (Myc-DDK-tagged)-Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1)
ORF Plasmid: tGFP
"NM_006516" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 6 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CSE; DYT9; DYT17; DYT18; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222696 representing NM_006516
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCAGCAGCAAGAAGCTGACGGGTCGCCTCATGCTGGCCGTGGGAGGAGCAGTGCTTGGCTCCC TGCAGTTTGGCTACAACACTGGAGTCATCAATGCCCCCCAGAAGGTGATCGAGGAGTTCTACAACCAGAC ATGGGTCCACCGCTATGGGGAGAGCATCCTGCCCACCACGCTCACCACGCTCTGGTCCCTCTCAGTGGCC ATCTTTTCTGTTGGGGGCATGATTGGCTCCTTCTCTGTGGGCCTTTTCGTTAACCGCTTTGGCCGGCGGA ATTCAATGCTGATGATGAACCTGCTGGCCTTCGTGTCCGCCGTGCTCATGGGCTTCTCGAAACTGGGCAA GTCCTTTGAGATGCTGATCCTGGGCCGCTTCATCATCGGTGTGTACTGCGGCCTGACCACAGGCTTCGTG CCCATGTATGTGGGTGAAGTGTCACCCACAGCCCTTCGTGGGGCCCTGGGCACCCTGCACCAGCTGGGCA TCGTCGTCGGCATCCTCATCGCCCAGGTGTTCGGCCTGGACTCCATCATGGGCAACAAGGACCTGTGGCC CCTGCTGCTGAGCATCATCTTCATCCCGGCCCTGCTGCAGTGCATCGTGCTGCCCTTCTGCCCCGAGAGT CCCCGCTTCCTGCTCATCAACCGCAACGAGGAGAACCGGGCCAAGAGTGTGCTAAAGAAGCTGCGCGGGA CAGCTGACGTGACCCATGACCTGCAGGAGATGAAGGAAGAGAGTCGGCAGATGATGCGGGAGAAGAAGGT CACCATCCTGGAGCTGTTCCGCTCCCCCGCCTACCGCCAGCCCATCCTCATCGCTGTGGTGCTGCAGCTG TCCCAGCAGCTGTCTGGCATCAACGCTGTCTTCTATTACTCCACGAGCATCTTCGAGAAGGCGGGGGTGC AGCAGCCTGTGTATGCCACCATTGGCTCCGGTATCGTCAACACGGCCTTCACTGTCGTGTCGCTGTTTGT GGTGGAGCGAGCAGGCCGGCGGACCCTGCACCTCATAGGCCTCGCTGGCATGGCGGGTTGTGCCATACTC ATGACCATCGCGCTAGCACTGCTGGAGCAGCTACCCTGGATGTCCTATCTGAGCATCGTGGCCATCTTTG GCTTTGTGGCCTTCTTTGAAGTGGGTCCTGGCCCCATCCCATGGTTCATCGTGGCTGAACTCTTCAGCCA GGGTCCACGTCCAGCTGCCATTGCCGTTGCAGGCTTCTCCAACTGGACCTCAAATTTCATTGTGGGCATG TGCTTCCAGTATGTGGAGCAACTGTGTGGTCCCTACGTCTTCATCATCTTCACTGTGCTCCTGGTTCTGT TCTTCATCTTCACCTACTTCAAAGTTCCTGAGACTAAAGGCCGGACCTTCGATGAGATCGCTTCCGGCTT CCGGCAGGGGGGAGCCAGCCAAAGTGACAAGACACCCGAGGAGCTGTTCCATCCCCTGGGGGCTGATTCC CAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222696 representing NM_006516
Red=Cloning site Green=Tags(s) MEPSSKKLTGRLMLAVGGAVLGSLQFGYNTGVINAPQKVIEEFYNQTWVHRYGESILPTTLTTLWSLSVA IFSVGGMIGSFSVGLFVNRFGRRNSMLMMNLLAFVSAVLMGFSKLGKSFEMLILGRFIIGVYCGLTTGFV PMYVGEVSPTALRGALGTLHQLGIVVGILIAQVFGLDSIMGNKDLWPLLLSIIFIPALLQCIVLPFCPES PRFLLINRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQL SQQLSGINAVFYYSTSIFEKAGVQQPVYATIGSGIVNTAFTVVSLFVVERAGRRTLHLIGLAGMAGCAIL MTIALALLEQLPWMSYLSIVAIFGFVAFFEVGPGPIPWFIVAELFSQGPRPAAIAVAGFSNWTSNFIVGM CFQYVEQLCGPYVFIIFTVLLVLFFIFTYFKVPETKGRTFDEIASGFRQGGASQSDKTPEELFHPLGADS QV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006516 |
| ORF Size | 1476 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006516.4 |
| RefSeq Size | 2856 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 6513 |
| UniProt ID | P11166 |
| Domains | sugar_tr |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Adipocytokine signaling pathway, Pathways in cancer, Renal cell carcinoma |
| MW | 53.9 kDa |
| Gene Summary | This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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LncRNA DUXAP8 facilitates multiple malignant phenotypes and resistance to PARP inhibitor in hepatocellular carcinoma via upregulating FOXM1
,Hu, Y;Zhang, X;Zai, H;Jiang, W;Xiao, L;Zhu, Q;,
Molecular Therapy - Oncolytics
,PubMed ID 33313387
[Glucose Transporter GLUT1]
|
|
CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis
,Camus, SM;Camus, MD;Figueras-Novoa, C;Boncompain, G;Sadacca, LA;Esk, C;Bigot, A;Gould, GW;Kioumourtzoglou, D;Perez, F;Bryant, NJ;Mukherjee, S;Brodsky, FM;,
J. Cell Biol.
,PubMed ID 31863584
[Glucose Transporter GLUT1]
|
|
Gene therapy for Glut1-deficient mouse using an adeno-associated virus vector with the human intrinsic GLUT1 promoter
,Nakamura, S;Muramatsu, SI;Takino, N;Ito, M;Jimbo, EF;Shimazaki, K;Onaka, T;Ohtsuki, S;Terasaki, T;Yamagata, T;Osaka, H;,
J Gene Med
,PubMed ID 29624790
[Glucose Transporter GLUT1]
|
|
Combination of temozolomide and Taxol exerts a synergistic inhibitory effect on Taxol‑resistant glioma cells via inhibition of glucose metabolism.
,null,
Molecular medicine reports
,PubMed ID 26459853
[Glucose Transporter GLUT1]
|
|
Mutational and functional analysis of Glucose transporter I deficiency syndrome
,Nakamura, S;Osaka, H;Muramatsu, S;Aoki, S;Jimbo, EF;Yamagata, T;,
Mol. Genet. Metab.
,PubMed ID 26304067
[Glucose Transporter GLUT1]
|
|
Re-sensitization of 5-FU resistance by SPARC through negative regulation of glucose metabolism in hepatocellular carcinoma
,Hua, HW;Jiang, F;Huang, Q;Liao, ZJ;Ding, G;,
Tumour Biol.
,PubMed ID 25252848
[Glucose Transporter GLUT1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222696L1 | Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222696L2 | Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1), mGFP tagged |
CNY 6,056.00 |
|
| RC222696L3 | Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222696L4 | Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1), mGFP tagged |
CNY 6,056.00 |
|
| RG222696 | SLC2A1 (tGFP-tagged) - Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1) |
CNY 5,256.00 |
|
| SC116011 | SLC2A1 (untagged)-Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1) |
CNY 5,488.00 |
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