TMEM16B (ANO2) (NM_020373) Human Tagged ORF Clone
CAT#: RC222601
ANO2 (Myc-DDK-tagged)-Human anoctamin 2 (ANO2)
ORF Plasmid: tGFP
"NM_020373" in other vectors (6)
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CNY 7,320.00
CNY 9,980.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C12orf3; TMEM16B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222601 representing NM_020373
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAAGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTCAGGCAGGGTCCAGAGGGG GCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAGGTCCCCGGGCCCCAGGTCTGCAGGGCGG TTCCAACAGAGATCCTGGCCAGCCCTGCGGTGGAGAGAGCACCCGCAGCAGCTCTGTCATCAACAACTAT CTGGATGCCAATGAGCCTGTGTCCTTGGAGGCCCGTCTTAGCCGCATGCACTTCCATGACAGTCAGAGGA AGGTCGACTATGTACTTGCCTACCACTACCGGAAACGCGGGGTGCACCTGGCCCAAGGCTTCCCTGGCCA CTCGCTGGCTATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGGGGCCCAGGTGACATTGAG CTGGGACCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAATTTGAGCACAATCTGATGG AGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCCAGGGATCCATCTTTGTCCGGATACACGC CCCGTGGCAGGTGCTGGCCAGAGAGGCAGAATTCTTGAAGATCAAAGTTCCTACCAAGAAAGAGATGTAC GAGATCAAAGCAGGAGGCAGCATTGCAAAGAAGTTCAGCGCGGCTCTGCAGAAGCTGAGCTCGCACCTGC AGCCCCGAGTTCCAGAACACAGCAACAACAAGATGAAAAACCTCTCCTACCCATTCTCCAGGGAGAAGAT GTACCTGTACAACATCCAGGAAAAGGACACCTTCTTTGATAATGCCACCCGTAGCCGCATTGTGCACGAG ATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGTATTAACTCTCTGATCGCAAACAATA TCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGTCCAGAGGACGATATGAATGACAGGAA GCTGCTATATCAAGAATGGGCGCGCTATGGAGTGTTCTATAAGTTCCAACCTATTGACCTCATCAGAAAG TATTTTGGAGAAAAAATTGGACTGTATTTTGCCTGGCTGGGATTATATACATCATTCCTCATCCCATCTT CTGTAATTGGAGTGATTGTGTTTCTTTATGGATGTGCAACAATTGAAGAAGATATTCCCAGCAGAGAGAT GTGTGACCAGCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGTGATTACTGGAACCTCAGC TCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGACAACCCTGCCACCGTCTTCTTCTCTATCTTCA TGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAGAGGCTACAGATGCGACTGGGCTACTTTTGGGA CCTGACTGGCATAGAAGAGGAAGAAGAACGTGCCCAGGAACATTCCAGGCCTGAGTATGAAACCAAAGTT CGAGAGAAAATGCTAAAGGAGAGCAACCAGTCTGCTGTCCAGAAATTGGAAACAAACACGACGGAGTGTG GCGATGAGGATGATGAAGATAAACTGACCTGGAAGGATCGTTTCCCAGGTTACCTGATGAACTTTGCCTC CATCTTATTCATGATTGCCCTGACATTCTCAATCGTCTTTGGGGTGATAGTGTATCGAATAACAACTGCA GCCGCTCTGTCTCTCAATAAGGCTACACGCTCCAATGTCCGGGTGACAGTGACAGCAACAGCAGTCATCA TCAACCTCGTGGTCATCCTCATCCTGGACGAGATCTACGGCGCTGTGGCCAAGTGGCTCACCAAAATTGA GGTTCCGAAAACAGAACAGACTTTTGAAGAGCGCCTGATCCTCAAAGCTTTCTTGCTCAAGTTTGTCAAT GCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAAGGGAGGTTTGTGGGCAGGCCTGGAAGCTACGTCT ATGTATTCGATGGTTACCGCATGGAAGAGTGTGCTCCAGGGGGCTGTCTCATGGAGCTCTGCATTCAGCT CAGCATCATCATGTTGGGGAAGCAGTTGATCCAGAACAACATCTTTGAGATTGGAGTCCCGAAGCTAAAG AAACTATTTCGAAAGCTGAAAGATGAGACCGAAGCTGGAGAAACTGACTCTGCCCATTCGAAACATCCAG AGCAGTGGGACCTAGACTACAGCTTGGAACCATACACAGGACTGACTCCGGAGTACATGGAAATGATCAT CCAGTTTGGTTTTGTCACCCTCTTCGTGGCCTCCTTTCCCCTGGCACCTGTGTTTGCCCTCCTCAACAAC GTCATTGAAGTGCGGCTCGATGCAAAGAAGTTTGTTACAGAGCTGAGACGGCCGGATGCTGTAAGAACCA AAGATATCGGAATCTGGTTTGACATTCTCTCTGGAATTGGCAAGTTCTCTGTTATCAGCAACGCTTTTGT CATTGCGATCACCTCCGACTTTATCCCCCGCCTGGTGTACCAGTACTCCTACAGTCACAATGGGACTCTG CACGGCTTTGTCAACCACACCCTCTCCTTTTTCAACGTCAGCCAGCTGAAGGAGGGGACGCAGCCAGAAA ACTCACAGTTTGACCAGGAGGTTCAGTTCTGCAGGTTTAAGGATTACCGAGAGCCGCCATGGGCCCCGAA CCCTTATGAGTTTTCGAAACAGTACTGGTTTATTCTGTCTGCCCGTCTGGCTTTTGTCATAATCTTCCAG AACCTCGTGATGTTCCTGAGCGTCCTCGTGGACTGGATGATTCCAGACATCCCCACGGACATCAGCGACC AGATCAAGAAAGAGAAGAGCTTATTAGTGGATTTCTTCCTGAAAGAGGAGCATGAGAAGCTCAAGCTGAT GGATGAGCCGGCTCTGAGGAGCCCAGGAGGTGGGGATCGAAGCAGGAGCCGGGCAGCCAGCTCAGCACCT TCAGGCCAAAGCCAGCTGGGCAGCATGATGTCGTCAGGCTCTCAGCACACCAATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222601 representing NM_020373
Red=Cloning site Green=Tags(s) MPEDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGESTRSSSVINNY LDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLAIVSNGETGKEPHAGGPGDIE LGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGSIFVRIHAPWQVLAREAEFLKIKVPTKKEMY EIKAGGSIAKKFSAALQKLSSHLQPRVPEHSNNKMKNLSYPFSREKMYLYNIQEKDTFFDNATRSRIVHE ILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPEDDMNDRKLLYQEWARYGVFYKFQPIDLIRK YFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSREMCDQQNAFTMCPLCDKSCDYWNLS SACGTAQASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQEHSRPEYETKV REKMLKESNQSAVQKLETNTTECGDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGVIVYRITTA AALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKFVN AYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLK KLFRKLKDETEAGETDSAHSKHPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNN VIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVISNAFVIAITSDFIPRLVYQYSYSHNGTL HGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWFILSARLAFVIIFQ NLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLKLMDEPALRSPGGGDRSRSRAASSAP SGQSQLGSMMSSGSQHTNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020373 |
ORF Size | 2997 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020373.1, NP_065106.1 |
RefSeq Size | 3717 bp |
RefSeq ORF | 2996 bp |
Locus ID | 57101 |
Protein Families | Transmembrane |
MW | 113.3 kDa |
Gene Summary | ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
The Calcium-Activated Chloride Channel TMEM16A is Inhibitied by Liquiritigenin
,null,
Frontiers in Pharmacology
,PubMed ID 33897420
[TMEM16B]
|
Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2
,Seo, Y;Lee, HK;Park, J;Jeon, DK;Jo, S;Jo, M;Namkung, W;,
PLoS ONE
,PubMed ID 27219012
[TMEM16B]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222601L1 | Lenti ORF clone of Human anoctamin 2 (ANO2), Myc-DDK-tagged |
CNY 11,880.00 |
|
RC222601L2 | Lenti ORF clone of Human anoctamin 2 (ANO2), mGFP tagged |
CNY 11,880.00 |
|
RC222601L3 | Lenti ORF clone of Human anoctamin 2 (ANO2), Myc-DDK-tagged |
CNY 11,880.00 |
|
RC222601L4 | Lenti ORF clone of Human anoctamin 2 (ANO2), mGFP tagged |
CNY 9,720.00 |
|
RG222601 | ANO2 (tGFP-tagged) - Human anoctamin 2 (ANO2) |
CNY 8,920.00 |
|
SC304752 | ANO2 (untagged)-Human anoctamin 2 (ANO2) |
CNY 7,320.00 |
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