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FIGN (NM_018086) Human Tagged ORF Clone
CAT＃: RC222534
FIGN (Myc-DDK-tagged)-Human fidgetin (FIGN)
ORF Plasmid: tGFP
"NM_018086" in other vectors (4)
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CNY 5,560.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222534 representing NM_018086
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGTAGCACCAGTGTTTATGGCTTGAAGATGCAGTGGACGCCAGAGCATGCCCAGTGGCCAGAAC AGCACTTTGACATCACCTCAACCACTCGGTCTCCTGCCCACAAAGTTGAAGCCTACAGAGGTCATCTGCA GCGCACCTATCAGTACGCCTGGGCGAATGATGACATATCTGCTCTGACTGCATCCAACCTACTAAAAAAA TATGCAGAGAAGTATTCCGGCATTTTGGAAGGTCCTGTGGACCGACCCGTACTCAGCAACTATTCGGACA CACCATCAGGACTAGTGAACGGTCGGAAAAATGAAAGTGAACCCTGGCAGCCTTCCTTGAATTCAGAAGC TGTTTATCCCATGAACTGTGTTCCGGATGTTATCACTGCCAGCAAAGCTGGAGTCAGTTCAGCCCTCCCT CCAGCAGATGTCTCTGCGAGTATAGGAAGCTCTCCTGGGGTAGCCAGCAACCTGACAGAACCTAGTTATT CAAGTAGTACCTGTGGAAGCCACACTGTACCCAGTCTTCATGCAGGGCTCCCATCTCAGGAATATGCCCC AGGATACAACGGATCATATTTGCATTCTACTTATAGTAGCCAGCCAGCACCTGCACTTCCTTCACCTCAT CCGTCTCCTTTGCATAGCTCTGGGCTACTACAGCCCCCACCACCACCTCCTCCGCCACCAGCCTTGGTCC CAGGCTACAATGGGACTTCTAACCTCTCCAGTTACAGCTATCCGTCTGCTAGCTATCCTCCTCAGACTGC TGTGGGGTCTGGGTACAGCCCTGGGGGGGCACCGCCTCCGCCTTCAGCGTACCTGCCTTCAGGAATTCCT GCTCCCACCCCCCTACCCCCCACCACTGTTCCTGGCTACACCTACCAGGGCCATGGTTTGACACCTATTG CACCGTCGGCTCTGACAAACAGTTCAGCAAGTTCTCTCAAAAGGAAAGCTTTCTACATGGCAGGGCAAGG AGATATGGACTCCAGTTATGGAAATTACAGCTATGGCCAACAGAGATCTACACAGAGTCCTATGTACAGA ATGCCCGACAACAGCATTTCAAACACAAATCGGGGGAATGGCTTTGACAGAAGTGCTGAAACATCATCCT TAGCATTTAAGCCAACGAAGCAGCTAATGTCCTCTGAACAGCAAAGGAAATTCAGCAGCCAGTCCAGTAG GGCTCTGACCCCTCCTTCCTACAGTACTGCTAAAAATTCATTGGGATCAAGATCCAGTGAATCCTTTGGG AAGTACACATCGCCAGTAATGAGTGAGCATGGGGACGAGCACAGGCAGCTCCTCTCTCACCCAATGCAAG GCCCTGGACTCCGTGCAGCTACCTCATCCAACCACTCTGTGGACGAGCAACTGAAGAATACTGACACGCA CCTCATCGACCTGGTAACCAATGAGATTATCACCCAAGGACCTCCAGTGGACTGGAATGACATTGCTGGT CTCGACCTGGTGAAGGCTGTCATTAAAGAGGAGGTTTTATGGCCAGTGTTGAGGTCAGACGCGTTCAGTG GACTGACGGCCTTACCTCGGAGCATCCTTTTATTTGGACCTCGGGGGACAGGCAAAACATTATTGGGCAG ATGCATCGCTAGTCAGCTGGGGGCCACATTTTTCAAAATTGCCGGTTCTGGACTAGTCGCCAAGTGGTTA GGAGAAGCAGAGAAAATTATCCATGCCTCTTTTCTTGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTG TTAGTGACATTGACATGCTTCTCTCCTCTCAAGTGAATGAGGAACATAGTCCAGTCAGTCGGATGAGAAC CGAATTTCTGATGCAACTGGACACTGTACTAACTTCGGCTGAGGACCAAATCGTAGTAATTTGTGCCACC AGTAAACCAGAAGAAATAGATGAATCCCTTCGGAGGTACTTCATGAAACGACTTTTAATCCCACTTCCTG ACAGCACAGCGAGGCACCAGATAATAGTACAACTGCTCTCACAGCACAATTACTGTCTCAATGACAAGGA GTTTGCACTGCTCGTCCAGCGCACAGAAGGCTTTTCTGGACTAGATGTGGCTCATTTGTGTCAGGAAGCA GTGGTGGGCCCCCTCCATGCCATGCCAGCCACAGACCTTTCAGCCATTATGCCCAGCCAGTTGAGGCCCG TTACATATCAAGACTTTGAAAATGCTTTCTGCAAGATTCAGCCTAGCATATCTCAAAAGGAGCTTGATAT GTATGTTGAATGGAACAAAATGTTTGGTTGCAGTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC222534 representing NM_018086
Red=Cloning site Green=Tags(s) MISSTSVYGLKMQWTPEHAQWPEQHFDITSTTRSPAHKVEAYRGHLQRTYQYAWANDDISALTASNLLKK YAEKYSGILEGPVDRPVLSNYSDTPSGLVNGRKNESEPWQPSLNSEAVYPMNCVPDVITASKAGVSSALP PADVSASIGSSPGVASNLTEPSYSSSTCGSHTVPSLHAGLPSQEYAPGYNGSYLHSTYSSQPAPALPSPH PSPLHSSGLLQPPPPPPPPPALVPGYNGTSNLSSYSYPSASYPPQTAVGSGYSPGGAPPPPSAYLPSGIP APTPLPPTTVPGYTYQGHGLTPIAPSALTNSSASSLKRKAFYMAGQGDMDSSYGNYSYGQQRSTQSPMYR MPDNSISNTNRGNGFDRSAETSSLAFKPTKQLMSSEQQRKFSSQSSRALTPPSYSTAKNSLGSRSSESFG KYTSPVMSEHGDEHRQLLSHPMQGPGLRAATSSNHSVDEQLKNTDTHLIDLVTNEIITQGPPVDWNDIAG LDLVKAVIKEEVLWPVLRSDAFSGLTALPRSILLFGPRGTGKTLLGRCIASQLGATFFKIAGSGLVAKWL GEAEKIIHASFLVARCRQPSVIFVSDIDMLLSSQVNEEHSPVSRMRTEFLMQLDTVLTSAEDQIVVICAT SKPEEIDESLRRYFMKRLLIPLPDSTARHQIIVQLLSQHNYCLNDKEFALLVQRTEGFSGLDVAHLCQEA VVGPLHAMPATDLSAIMPSQLRPVTYQDFENAFCKIQPSISQKELDMYVEWNKMFGCSQ SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018086 |
| ORF Size | 2277 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018086.4 |
| RefSeq Size | 4535 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 55137 |
| UniProt ID | Q5HY92 |
| Domains | AAA |
| MW | 82 kDa |
| Gene Summary | ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, that may lead to the suppression of microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222534L3 | Lenti ORF clone of Human fidgetin (FIGN), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC222534L4 | Lenti ORF clone of Human fidgetin (FIGN), mGFP tagged |
CNY 7,700.00 |
|
| RG222534 | FIGN (tGFP-tagged) - Human fidgetin (FIGN) |
CNY 6,370.00 |
|
| SC314631 | FIGN (untagged)-Human fidgetin (FIGN) |
CNY 14,730.00 |
