United States
Germany
Japan
United Kingdom
China
PHC1 (NM_004426) Human Tagged ORF Clone
CAT＃: RC222530
PHC1 (Myc-DDK-tagged)-Human polyhomeotic homolog 1 (Drosophila) (PHC1)
ORF Plasmid: tGFP
"NM_004426" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,992.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EDR1; HPH1; MCPH11; RAE28 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222530 representing NM_004426
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGAGAGCGAGCAGAACTCCAATTCCACCAATGGGAGTTCTAGCTCAGGGGGCAGCTCTCGGC CCCAGATAGCTCAAATGTCACTATATGAACGACAAGCAGTGCAGGCTCTGCAAGCACTGCAGCGGCAGCC CAATGCAGCTCAGTATTTCCACCAGTTCATGCTCCAGCAGCAGCTCAGTAATGCCCAGCTGCATAGCCTG GCTGCCGTCCAGCAGGCCACAATTGCTGCCAGTCGGCAGGCCAGCTCCCCAAACACCAGCACTACACAGC AGCAGACTACCACCACCCAGGCCTCGATCAATCTGGCCACCACATCGGCCGCCCAGCTCATCAGCCGATC CCAGAGTGTGAGCTCTCCCAGTGCTACCACCTTGACCCAATCTGTGCTACTGGGGAACACCACCTCCCCA CCCCTCAACCAGTCTCAGGCCCAGATGTATCTACGGCCACAGCTGGGAAACCTATTGCAGGTAAACCGAA CCCTGGGTCGGAATGTGCCTCTAGCCTCCCAACTCATCCTGATGCCTAATGGGGCGGTGGCTGCAGTCCA GCAGGAGGTGCCATCTGCTCAGTCTCCTGGAGTTCATGCAGATGCAGATCAGGTTCAGAACTTGGCAGTA AGGAATCAACAGGCCTCAGCTCAAGGACCTCAGATGCAAGGCTCCACTCAGAAGGCCATTCCTCCAGGAG CCTCCCCTGTCTCTAGCCTCTCCCAGGCCTCTAGCCAGGCCCTAGCGGTGGCACAGGCTTCCTCTGGGGC CACAAACCAGTCCCTCAACCTTAGTCAAGCTGGTGGAGGCAGTGGGAATAGCATCCCAGGGTCCATGGGT CCAGGTGGAGGTGGGCAGGCACATGGTGGTTTGGGTCAGTTGCCTTCCTCAGGAATGGGTGGTGGGAGCT GTCCCAGGAAGGGTACAGGAGTGGTGCAGCCCTTGCCTGCAGCCCAAACAGTGACTGTGAGCCAGGGCAG CCAGACAGAGGCAGAAAGTGCAGCAGCCAAGAAGGCAGAAGCAGATGGGAGTGGCCAGCAGAATGTGGGC ATGAACCTGACACGGACAGCCACACCTGCGCCCAGCCAGACACTTATTAGCTCAGCCACCTACACACAGA TCCAGCCCCATTCACTGATTCAGCAACAGCAACAGATCCACCTCCAGCAGAAACAGGTGGTGATCCAGCA GCAGATTGCCATCCACCACCAGCAGCAGTTCCAGCACCGGCAGTCCCAGCTCCTTCACACAGCTACACAC CTCCAGTTGGCGCAGCAGCAGCAGCAGCAACAACAGCAACAGCAGCAACAGCAGCAGCCGCAAGCCACCA CCCTCACTGCCCCTCAGCCACCACAGGTCCCACCTACTCAGCAGGTCCCACCTTCCCAGTCCCAGCAGCA AGCCCAAACCCTGGTCGTTCAGCCCATGCTTCAGTCTTCACCCTTGTCTCTTCCACCTGATGCAGCCCCT AAGCCACCAATTCCCATCCAATCCAAACCACCTGTAGCACCTATCAAGCCGCCTCAGTTAGGGGCCGCTA AGATGTCAGCTGCCCAGCAACCACCACCCCATATCCCTGTGCAAGTTGTAGGCACTCGACAGCCAGGTAC AGCCCAGGCACAGGCTTTGGGGTTGGCACAGCTGGCAGCTGCTGTACCTACTTCCCGGGGGATGCCAGGT ACAGTGCAGTCTGGTCAGGCCCATTTGGCCTCCTCGCCACCTTCATCCCAGGCTCCTGGTGCACTGCAGG AGTGCCCTCCCACATTGGCCCCTGGGATGACCCTTGCTCCTGTGCAGGGGACAGCACATGTGGTAAAGGG TGGGGCTACCACCTCCTCACCTGTTGTAGCCCAGGTCCCTGCTGCCTTCTATATGCAGTCTGTGCACTTG CCGGGTAAACCCCAGACATTGGCTGTCAAACGCAAGGCTGACTCTGAGGAGGAGAGAGATGATGTCTCCA CATTGGGTTCAATGCTTCCTGCCAAGGCATCTCCAGTAGCAGAAAGCCCAAAAGTCATGGACGAGAAGAG CAGTCTTGGAGAAAAAGCTGAATCAGTGGCTAATGTGAATGCTAATACTCCAAGCAGTGAACTAGTAGCC TTGACCCCCGCCCCTTCAGTACCGCCTCCTACACTAGCCATGGTGTCTAGACAAATGGGTGACTCAAAAC CCCCACAGGCCATCGTGAAGCCCCAGATTCTCACCCACATCATTGAAGGCTTTGTTATCCAGGAAGGAGC AGAACCTTTCCCGGTGGGTTGTTCTCAGTTACTGAAGGAGTCTGAGAAGCCACTACAGACTGGCCTTCCG ACAGGGCTGACTGAGAATCAGTCAGGTGGCCCTTTGGGAGTGGACAGCCCATCTGCTGAGTTAGATAAGA AGGCGAATCTCCTGAAGTGCGAGTACTGTGGGAAGTACGCCCCCGCAGAGCAGTTTCGTGGCTCTAAGAG GTTCTGCTCCATGACTTGCGCTAAGAGGTACAATGTGAGCTGTAGCCATCAGTTCCGGCTGAAGAGGAAA AAAATGAAAGAGTTTCAAGAAGCCAACTATGCTCGCGTTCGCAGGCGTGGACCCCGCCGCAGCTCCTCTG ACATTGCCCGTGCCAAGATTCAGGGCAAGTGCCACCGGGGTCAAGAAGACTCTAGCCGGGGTTCAGATAA TTCCAGTTATGATGAAGCACTCTCTCCAACATCTCCTGGGCCTTTATCAGTAAGAGCTGGGCATGGAGAA CGTGACCTGGGGAATCCCAATACAGCTCCACCTACACCGGAATTACATGGCATCAACCCTGTGTTCCTGT CCAGTAATCCCAGCCGTTGGAGTGTAGAGGAGGTGTACGAGTTTATTGCTTCTCTCCAAGGCTGCCAAGA GATTGCAGAGGAATTTCGCTCACAGGAGATTGATGGACAGGCCCTTTTATTACTTAAAGAAGAACATCTT ATGAGTGCCATGAACATCAAGCTGGGCCCTGCCCTCAAGATCTGCGCCAAGATAAATGTCCTCAAGGAGA CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222530 representing NM_004426
Red=Cloning site Green=Tags(s) METESEQNSNSTNGSSSSGGSSRPQIAQMSLYERQAVQALQALQRQPNAAQYFHQFMLQQQLSNAQLHSL AAVQQATIAASRQASSPNTSTTQQQTTTTQASINLATTSAAQLISRSQSVSSPSATTLTQSVLLGNTTSP PLNQSQAQMYLRPQLGNLLQVNRTLGRNVPLASQLILMPNGAVAAVQQEVPSAQSPGVHADADQVQNLAV RNQQASAQGPQMQGSTQKAIPPGASPVSSLSQASSQALAVAQASSGATNQSLNLSQAGGGSGNSIPGSMG PGGGGQAHGGLGQLPSSGMGGGSCPRKGTGVVQPLPAAQTVTVSQGSQTEAESAAAKKAEADGSGQQNVG MNLTRTATPAPSQTLISSATYTQIQPHSLIQQQQQIHLQQKQVVIQQQIAIHHQQQFQHRQSQLLHTATH LQLAQQQQQQQQQQQQQQQPQATTLTAPQPPQVPPTQQVPPSQSQQQAQTLVVQPMLQSSPLSLPPDAAP KPPIPIQSKPPVAPIKPPQLGAAKMSAAQQPPPHIPVQVVGTRQPGTAQAQALGLAQLAAAVPTSRGMPG TVQSGQAHLASSPPSSQAPGALQECPPTLAPGMTLAPVQGTAHVVKGGATTSSPVVAQVPAAFYMQSVHL PGKPQTLAVKRKADSEEERDDVSTLGSMLPAKASPVAESPKVMDEKSSLGEKAESVANVNANTPSSELVA LTPAPSVPPPTLAMVSRQMGDSKPPQAIVKPQILTHIIEGFVIQEGAEPFPVGCSQLLKESEKPLQTGLP TGLTENQSGGPLGVDSPSAELDKKANLLKCEYCGKYAPAEQFRGSKRFCSMTCAKRYNVSCSHQFRLKRK KMKEFQEANYARVRRRGPRRSSSDIARAKIQGKCHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAGHGE RDLGNPNTAPPTPELHGINPVFLSSNPSRWSVEEVYEFIASLQGCQEIAEEFRSQEIDGQALLLLKEEHL MSAMNIKLGPALKICAKINVLKET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004426 |
| ORF Size | 3012 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004426.2, NP_004417.2 |
| RefSeq Size | 5206 bp |
| RefSeq ORF | 3015 bp |
| Locus ID | 1911 |
| UniProt ID | P78364 |
| Domains | SAM |
| Protein Families | ES Cell Differentiation/IPS, Stem cell - Pluripotency |
| MW | 105.4 kDa |
| Gene Summary | This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222530L1 | Lenti ORF clone of Human polyhomeotic homolog 1 (Drosophila) (PHC1), Myc-DDK-tagged |
CNY 9,392.00 |
|
| RC222530L2 | Lenti ORF clone of Human polyhomeotic homolog 1 (Drosophila) (PHC1), mGFP tagged |
CNY 12,540.00 |
|
| RC222530L3 | Lenti ORF clone of Human polyhomeotic homolog 1 (Drosophila) (PHC1), Myc-DDK-tagged |
CNY 12,540.00 |
|
| RC222530L4 | Lenti ORF clone of Human polyhomeotic homolog 1 (Drosophila) (PHC1), mGFP tagged |
CNY 12,540.00 |
|
| RG222530 | PHC1 (tGFP-tagged) - Human polyhomeotic homolog 1 (Drosophila) (PHC1) |
CNY 8,592.00 |
|
| SC127500 | PHC1 (untagged)-Human polyhomeotic homolog 1 (Drosophila) (PHC1) |
CNY 7,000.00 |
