C18orf1 (LDLRAD4) (NM_181481) Human Tagged ORF Clone
CAT#: RC222441
- TrueORF®
LDLRAD4 (Myc-DDK-tagged)-Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1
ORF Plasmid: tGFP
"NM_181481" in other vectors (6)
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CNY 3,600.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C18orf1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222441 representing NM_181481
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCTTTCACAGAGTGCAAATTCACCTGCACCAGTGGTAAAT GCTTGTATCTTGGTTCGCTGGTCTGTAACCAACAGAACGACTGTGGGGACAACAGTGACGAAGAGAACTG TCTCCTGGTGACCGAGCACCCGCCTCCGGGCATCTTCAACTCGGAGCTGGAGTTCGCCCAAATCATCATC ATCGTCGTGGTGGTCACGGTGATGGTGGTGGTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGC GGTCCTTCATCAACCGCCCGAACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAGGAAGGGTGCCTGTG GCCTTCAGACAGCGCCGCACCGCGGCTGGGCGCCTCGGAGATCATGCATGCCCCGCGGTCCAGGGACAGG TTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACCTACCCCTATGTGCAGC ACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAAGAGCCACCTCCTTACCAGGGGCCCTG CACCCTGCAGCTCCGGGACCCTGAACAGCAGATGGAACTCAACCGAGAGTCCGTGAGGGCCCCACCCAAC CGAACCATATTTGACAGTGATTTAATAGACATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCA ACTCGGGCATCAGTGCAAGCACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGA GGTGATGGGCCACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGCAGCAGA CTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAAGATAGGAAGCCTGGGA ACCTGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222441 representing NM_181481
Red=Cloning site Green=Tags(s) MPEAGFQATNAFTECKFTCTSGKCLYLGSLVCNQQNDCGDNSDEENCLLVTEHPPPGIFNSELEFAQIII IVVVVTVMVVVIVCLLNHYKVSTRSFINRPNQSRRREDGLPQEGCLWPSDSAAPRLGASEIMHAPRSRDR FTAPSFIQRDRFSRFQPTYPYVQHEIDLPPTISLSDGEEPPPYQGPCTLQLRDPEQQMELNRESVRAPPN RTIFDSDLIDIAMYSGGPCPPSSNSGISASTCSSNGRMEGPPPTYSEVMGHHPGASFLHHQRSNAHRGSR LQFQQNNAESTIVPIKGKDRKPGNLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_181481 |
ORF Size | 918 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181481.5 |
RefSeq Size | 8676 bp |
RefSeq ORF | 921 bp |
Locus ID | 753 |
UniProt ID | O15165 |
Protein Families | Druggable Genome, Transmembrane |
MW | 33.7 kDa |
Gene Summary | Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222441L1 | Lenti ORF clone of Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC222441L2 | Lenti ORF clone of Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1, mGFP tagged |
CNY 5,890.00 |
|
RC222441L3 | Lenti ORF clone of Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222441L4 | Lenti ORF clone of Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1, mGFP tagged |
CNY 5,890.00 |
|
RG222441 | LDLRAD4 (tGFP-tagged) - Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1 |
CNY 5,200.00 |
|
SC312881 | LDLRAD4 (untagged)-Human chromosome 18 open reading frame 1 (C18orf1), transcript variant a1 |
CNY 6,270.00 |
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