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KIR2.3 (KCNJ4) (NM_004981) Human Tagged ORF Clone
CAT＃: RC222272
KCNJ4 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2
ORF Plasmid: tGFP
"NM_004981" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HIR; HIRK2; HRK1; IRK-3; IRK3; Kir2.3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222272 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGGACACAGCCGCAACGGCCAGGCCCACGTGCCCCGGCGGAAGCGCCGCAACCGCTTCGTCAAGA AGAACGGCCAATGCAACGTGTACTTCGCCAACCTGAGCAACAAGTCGCAGCGCTACATGGCGGACATCTT CACCACCTGCGTGGACACGCGCTGGCGCTACATGCTCATGATCTTCTCCGCGGCCTTCCTTGTCTCCTGG CTCTTTTTCGGCCTCCTCTTCTGGTGTATCGCCTTCTTCCACGGTGACCTGGAGGCCAGCCCAGGGGTGC CTGCGGCGGGGGGCCCGGCGGCGGGTGGTGGCGGAGCAGCCCCGGTGGCCCCCAAGCCCTGCATCATGCA CGTGAACGGCTTCCTGGGTGCCTTCCTGTTCTCGGTGGAGACGCAGACGACCATCGGCTATGGGTTCCGG TGCGTGACAGAGGAGTGCCCGCTGGCAGTCATCGCTGTGGTGGTCCAGTCCATCGTGGGCTGCGTCATCG ACTCCTTCATGATTGGCACCATCATGGCCAAGATGGCGCGGCCCAAGAAGCGGGCGCAGACGTTGCTGTT CAGCCACCACGCGGTCATTTCGGTGCGCGACGGCAAGCTCTGCCTCATGTGGCGCGTGGGCAACCTGCGC AAGAGCCACATTGTGGAGGCCCACGTGCGGGCCCAGCTCATCAAGCCCTACATGACCCAGGAGGGCGAGT ACCTGCCCCTGGACCAGCGGGACCTCAACGTGGGCTATGACATCGGCCTGGACCGCATCTTCCTGGTGTC GCCCATCATCATTGTCCACGAGATCGACGAGGACAGCCCGCTTTATGGCATGGGCAAGGAGGAGCTGGAG TCGGAGGACTTTGAGATCGTGGTCATCCTGGAGGGCATGGTGGAGGCCACGGCCATGACCACCCAGGCCC GCAGCTCCTACCTGGCCAGCGAGATCCTGTGGGGCCACCGCTTTGAGCCTGTGGTCTTCGAGGAGAAGAG CCACTACAAGGTGGACTACTCGCGTTTTCACAAGACCTACGAGGTGGCCGGCACGCCCTGCTGCTCGGCC CGGGAGCTGCAGGAGAGTAAGATCACCGTGCTGCCCGCCCCACCGCCCCCTCCCAGTGCCTTCTGCTACG AGAACGAGCTGGCCCTTATGAGCCAGGAGGAAGAGGAGATGGAGGAGGAGGCAGCTGCGGCGGCCGCGGT GGCCGCAGGCCTGGGCCTGGAGGCGGGTTCCAAGGAGGAGGCGGGCATCATCCGGATGCTGGAGTTCGGC AGCCACCTGGACCTGGAGCGCATGCAGGCTTCCCTCCCGCTGGACAACATCTCCTACCGCAGGGAGTCTG CCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222272 protein sequence
Red=Cloning site Green=Tags(s) MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSW LFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFR CVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLR KSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELE SEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSA RELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFG SHLDLERMQASLPLDNISYRRESAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004981 |
| ORF Size | 1335 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004981.1, NP_004972.1 |
| RefSeq Size | 1913 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 3761 |
| UniProt ID | P48050 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 49.5 kDa |
| Gene Summary | Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222272L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222272L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2, mGFP tagged |
CNY 7,888.00 |
|
| RC222272L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222272L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG222272 | KCNJ4 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2 |
CNY 7,088.00 |
|
| SC123960 | KCNJ4 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2 |
CNY 5,488.00 |
