OATP2 (SLCO1B1) (NM_006446) Human Tagged ORF Clone
CAT#: RC222130
SLCO1B1 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1)
ORF Plasmid: tGFP
"NM_006446" in other vectors (6)
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CNY 5,064.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HBLRR; LST-1; LST1; OATP-C; OATP1B1; OATP2; OATPC; SLC21A6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222130 representing NM_006446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAAAATCAACATTTGAATAAAACAGCAGAGGCACAACCTTCAGAGAATAAGAAAACAAGATACT GCAATGGATTGAAGATGTTCTTGGCAGCTCTGTCACTCAGCTTTATTGCTAAGACACTAGGTGCAATTAT TATGAAAAGTTCCATCATTCATATAGAACGGAGATTTGAGATATCCTCTTCTCTTGTTGGTTTTATTGAC GGAAGCTTTGAAATTGGAAATTTGCTTGTGATTGTATTTGTGAGTTACTTTGGATCCAAACTACATAGAC CAAAGTTAATTGGAATCGGTTGTTTCATTATGGGAATTGGAGGTGTTTTGACTGCTTTGCCACATTTCTT CATGGGATATTACAGGTATTCTAAAGAAACTAATATCGATTCATCAGAAAATTCAACATCGACCTTATCC ACTTGTTTAATTAATCAAATTTTATCACTCAATAGAGCATCACCTGAGATAGTGGGAAAAGGTTGTTTAA AGGAATCTGGGTCATACATGTGGATATATGTGTTCATGGGTAATATGCTTCGTGGAATAGGGGAGACTCC CATAGTACCATTGGGGCTTTCTTACATTGATGATTTCGCTAAAGAAGGACATTCTTCTTTGTATTTAGGT ATATTGAATGCAATAGCAATGATTGGTCCAATCATTGGCTTTACCCTGGGATCTCTGTTTTCTAAAATGT ACGTGGATATTGGATATGTAGATCTAAGCACTATCAGGATAACTCCTACTGATTCTCGATGGGTTGGAGC TTGGTGGCTTAATTTCCTTGTGTCTGGACTATTCTCCATTATTTCTTCCATACCATTCTTTTTCTTGCCC CAAACTCCAAATAAACCACAAAAAGAAAGAAAAGCTTCACTGTCTTTGCATGTGCTGGAAACAAATGATG AAAAGGATCAAACAGCTAATTTGACCAATCAAGGAAAAAATATTACCAAAAATGTGACTGGTTTTTTCCA GTCTTTTAAAAGCATCCTTACTAATCCCCTGTATGTTATGTTTGTGCTTTTGACGTTGTTACAAGTAAGC AGCTATATTGGTGCTTTTACTTATGTCTTCAAATACGTAGAGCAACAGTATGGTCAGCCTTCATCTAAGG CTAACATCTTATTGGGAGTCATAACCATACCTATTTTTGCAAGTGGAATGTTTTTAGGAGGATATATCAT TAAAAAATTCAAACTGAACACCGTTGGAATTGCCAAATTCTCATGTTTTACTGCTGTGATGTCATTGTCC TTTTACCTATTATATTTTTTCATACTCTGTGAAAACAAATCAGTTGCCGGACTAACCATGACCTATGATG GAAATAATCCAGTGACATCTCATAGAGATGTACCACTTTCTTATTGCAACTCAGACTGCAATTGTGATGA AAGTCAATGGGAACCAGTCTGTGGAAACAATGGAATAACTTACATCTCACCCTGTCTAGCAGGTTGCAAA TCTTCAAGTGGCAATAAAAAGCCTATAGTGTTTTACAACTGCAGTTGTTTGGAAGTAACTGGTCTCCAGA ACAGAAATTACTCAGCCCATTTGGGTGAATGCCCAAGAGATGATGCTTGTACAAGGAAATTTTACTTTTT TGTTGCAATACAAGTCTTGAATTTATTTTTCTCTGCACTTGGAGGCACCTCACATGTCATGCTGATTGTT AAAATTGTTCAACCTGAATTGAAATCACTTGCACTGGGTTTCCACTCAATGGTTATACGAGCACTAGGAG GAATTCTAGCTCCAATATATTTTGGGGCTCTGATTGATACAACGTGTATAAAGTGGTCCACCAACAACTG TGGCACACGTGGGTCATGTAGGACATATAATTCCACATCATTTTCAAGGGTCTACTTGGGCTTGTCTTCA ATGTTAAGAGTCTCATCACTTGTTTTATATATTATATTAATTTATGCCATGAAGAAAAAATATCAAGAGA AAGATATCAATGCATCAGAAAATGGAAGTGTCATGGATGAAGCAAACTTAGAATCCTTAAATAAAAATAA ACATTTTGTCCCTTCTGCTGGGGCAGATAGTGAAACACATTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222130 representing NM_006446
Red=Cloning site Green=Tags(s) MDQNQHLNKTAEAQPSENKKTRYCNGLKMFLAALSLSFIAKTLGAIIMKSSIIHIERRFEISSSLVGFID GSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCFIMGIGGVLTALPHFFMGYYRYSKETNIDSSENSTSTLS TCLINQILSLNRASPEIVGKGCLKESGSYMWIYVFMGNMLRGIGETPIVPLGLSYIDDFAKEGHSSLYLG ILNAIAMIGPIIGFTLGSLFSKMYVDIGYVDLSTIRITPTDSRWVGAWWLNFLVSGLFSIISSIPFFFLP QTPNKPQKERKASLSLHVLETNDEKDQTANLTNQGKNITKNVTGFFQSFKSILTNPLYVMFVLLTLLQVS SYIGAFTYVFKYVEQQYGQPSSKANILLGVITIPIFASGMFLGGYIIKKFKLNTVGIAKFSCFTAVMSLS FYLLYFFILCENKSVAGLTMTYDGNNPVTSHRDVPLSYCNSDCNCDESQWEPVCGNNGITYISPCLAGCK SSSGNKKPIVFYNCSCLEVTGLQNRNYSAHLGECPRDDACTRKFYFFVAIQVLNLFFSALGGTSHVMLIV KIVQPELKSLALGFHSMVIRALGGILAPIYFGALIDTTCIKWSTNNCGTRGSCRTYNSTSFSRVYLGLSS MLRVSSLVLYIILIYAMKKKYQEKDINASENGSVMDEANLESLNKNKHFVPSAGADSETHC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006446 |
ORF Size | 2073 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006446.3 |
RefSeq Size | 2830 bp |
RefSeq ORF | 2076 bp |
Locus ID | 10599 |
UniProt ID | Q9Y6L6 |
Domains | OATP_N, OATP_C |
Protein Families | Druggable Genome, Transmembrane |
MW | 76.3 kDa |
Gene Summary | This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and leukotriene C4. This protein is also involved in the removal of drug compounds such as statins, bromosulfophthalein and rifampin from the blood into the hepatocytes. Polymorphisms in the gene encoding this protein are associated with impaired transporter function. [provided by RefSeq, Mar 2009] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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Characterization of Plasma Membrane Localization and Phosphorylation Status of Organic Anion Transporting Polypeptide (OATP) 1B1 c.521 T>C Nonsynonymous Single-Nucleotide Polymorphism
,Crowe, A;Zheng, W;Miller, J;Pahwa, S;Alam, K;Fung, KM;Rubin, E;Yin, F;Ding, K;Yue, W;,
Pharm. Res.
,PubMed ID 31093828
[OATP2]
|
A phosphotyrosine switch regulates organic cation transporters
,Sprowl, JA;Ong, SS;Gibson, AA;Hu, S;Du, G;Lin, W;Li, L;Bharill, S;Ness, RA;Stecula, A;Offer, SM;Diasio, RB;Nies, AT;Schwab, M;Cavaletti, G;Schlatter, E;Ciarimboli, G;Schellens, JH;Isacoff, EY;Sali, A;Chen, T;Baker, SD;Sparreboom, A;Pabla, N;,
Nat Commun
,PubMed ID 26979622
[OATP2]
|
Down-Regulation of Organic Anion Transporting Polypeptide (OATP) 1B1 Transport Function by Lysosomotropic Drug Chloroquine
,Alam, K;Pahwa, S;Wang, X;Zhang, P;Ding, K;,
Molecular pharmarceutics
,PubMed ID 26750564
[OATP2]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222130L1 | Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222130L2 | Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), mGFP tagged |
CNY 7,464.00 |
|
RC222130L3 | Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), Myc-DDK-tagged |
CNY 7,464.00 |
|
RC222130L4 | Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), mGFP tagged |
CNY 7,464.00 |
|
RG222130 | SLCO1B1 (tGFP-tagged) - Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1) |
CNY 6,664.00 |
|
SC116071 | SLCO1B1 (untagged)-Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1) |
CNY 7,600.00 |