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Cytokeratin 2 (KRT76) (NM_015848) Human Tagged ORF Clone
CAT＃: RC222015
KRT76 (Myc-DDK-tagged)-Human keratin 76 (KRT76)
ORF Plasmid: tGFP
"NM_015848" in other vectors (4)
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CNY 4,752.00
CNY 7,505.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HUMCYT2A; KRT2B; KRT2P |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222015 representing NM_015848
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAGACAAGTTTGCAAGAAATCCTTCAGTGGCAGGAGCCAGGGTTTCTCTGGCCGCTCTGCTGTGG TCTCCGGCAGCAGCAGGATGAGCTGTGTGGCCCGCTCTGGGGGAGCTGGTGGAGGGGCCTGTGGCTTCAG GAGCGGAGCAGGCAGCTTTGGCAGTCGCAGCCTCTACAACCTGGGCAGCAACAAGAGCATCTCCATCAGC GTGGCAGCTGGCAGCTCCCGGGCTGGAGGCTTTGGGGGAGGGCGGAGCAGCTGTGGCTTTGCAGGTGGCT ATGGAGGTGGCTTTGGGGGCAGCTATGGTGGTGGCTTTGGTGGTGGCAGAGGAGTAGGTAGTGGTTTTGG AGGGGCTGGTGGCTTTGGTGGAGCTGGTGGCTTTGGTGGTCCTGGTGTATTTGGTGGGCCTGGCAGCTTT GGTGGTCCTGGTGGCTTTGGCCCTGGGGGCTTTCCTGGGGGAATTCAGGAAGTGATTGTAAACCAGAGTC TCCTGCAGCCCCTCAATGTGGAGATCGACCCCCAGATTGGGCAAGTAAAGGCCCAGGAGCGGGAACAGAT CAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTCCTGGAACAGCAGAACAAGGTC CTGGAGACCAAGTGGGAACTGCTCCAGCAGCAGACCACAGGCTCAGGGCCCAGCAGCCTGGAGCCTTGTT TTGAATCCTACATCAGCTTCCTATGCAAGCAGCTAGATTCACTTCTAGGGGAGAGGGGGAACCTGGAGGG AGAGCTGAAAAGCATGCAGGACCTGGTGGAAGACTTCAAAAAGAAGTATGAAGATGAAATCAACAAACGC ACTGCCGCAGAGAATGAGTTTGTGGGGCTCAAGAAGGATGTGGATGCGGCTTTCATGAACAAGGTGGAGC TGCAGGCCAAAGTGGACAGCCTGACAGATGAAGTCAGCTTCCTGAGGACCCTCTATGAGATGGAGCTGTC CCAGATGCAAAGCCATGCCAGTGACACGTCTGTGGTTCTGTCCATGGACAACAACCGCTGCCTGGACCTG GGCAGCATCATTGCCGAGGTCCGCGCCCAGTATGAGGAGATTGCCCAGAGGAGCAAGTCTGAAGCTGAGG CCCTGTACCAGACCAAGCTTGGGGAGCTGCAGACCACAGCTGGCAGGCATGGGGATGACCTGAGGAACAC CAAGAGTGAGATCATGGAGCTCAACAGGATGATCCAGAGGCTACGGGCTGAGATTGAAAATGTCAAGAAG CAGAATGCCAACCTGCAGACGGCAATTGCAGAGGCTGAGCAGCGTGGAGAGATGGCCCTCAAGGACGCCA ATGCCAAGCTCCAAGACTTGCAGACTGCCCTACAGAAGGCTAAGGATGACCTGGCTCGGCTCCTGCGTGA CTACCAGGAGCTGATGAACGTCAAGCTGGCCCTGGATGTGGAGATTGCCACCTACCGCAAGCTGCTGGAG GGAGAGGAGTGCAGGATGTCTGGAGAATGTCAGAGTGCCGTGTGCATTTCAGTGGTCAGCAATGTCACCA GCACAAGTGGCAGCTCTGGCAGTAGCCGTGGAGTTTTTGGAGGGGTCAGTGGCAGTGGCAGTGGTGGCTA CAAAGGCGGCAGCAGCAGCAGCAGCAGCAGTGGCTATGGAGTCAGTGGCGGCAGCGGCAGTGGCTATGGA GGGGTCAGCAGTGGCAGCACTGGGGGCAGGGGTAGCAGCGGGAGCTACCAGAGCAGCAGTAGTGGGAGCA GGCTCGGCGGTGCAGGTAGCATCTCCGTGAGCCACAGTGGAATGGGCTCCAGCTCTGGCAGCATCCAGAC TTCTGGAGGAAGTGGCTACAAGTCTGGTGGTGGAGGCAGCACCAGTATCCGCTTCTCCCAGACCACGAGC TCAAGCCAGCATAGCTCCACCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222015 representing NM_015848
Red=Cloning site Green=Tags(s) MNRQVCKKSFSGRSQGFSGRSAVVSGSSRMSCVARSGGAGGGACGFRSGAGSFGSRSLYNLGSNKSISIS VAAGSSRAGGFGGGRSSCGFAGGYGGGFGGSYGGGFGGGRGVGSGFGGAGGFGGAGGFGGPGVFGGPGSF GGPGGFGPGGFPGGIQEVIVNQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKV LETKWELLQQQTTGSGPSSLEPCFESYISFLCKQLDSLLGERGNLEGELKSMQDLVEDFKKKYEDEINKR TAAENEFVGLKKDVDAAFMNKVELQAKVDSLTDEVSFLRTLYEMELSQMQSHASDTSVVLSMDNNRCLDL GSIIAEVRAQYEEIAQRSKSEAEALYQTKLGELQTTAGRHGDDLRNTKSEIMELNRMIQRLRAEIENVKK QNANLQTAIAEAEQRGEMALKDANAKLQDLQTALQKAKDDLARLLRDYQELMNVKLALDVEIATYRKLLE GEECRMSGECQSAVCISVVSNVTSTSGSSGSSRGVFGGVSGSGSGGYKGGSSSSSSSGYGVSGGSGSGYG GVSSGSTGGRGSSGSYQSSSSGSRLGGAGSISVSHSGMGSSSGSIQTSGGSGYKSGGGGSTSIRFSQTTS SSQHSSTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015848 |
| ORF Size | 1914 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015848.4, NP_056932.2 |
| RefSeq Size | 2513 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 51350 |
| UniProt ID | Q01546 |
| MW | 65.7 kDa |
| Gene Summary | Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222015L3 | Lenti ORF clone of Human keratin 76 (KRT76), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222015L4 | Lenti ORF clone of Human keratin 76 (KRT76), mGFP tagged |
CNY 5,890.00 |
|
| RG222015 | KRT76 (tGFP-tagged) - Human keratin 76 (KRT76) |
CNY 4,370.00 |
|
| SC304357 | KRT76 (untagged)-Human keratin 76 (KRT76) |
CNY 10,260.00 |
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