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SPFH2 (ERLIN2) (NM_001003790) Human Tagged ORF Clone
CAT＃: RC221757
- TrueORF®
ERLIN2 (Myc-DDK-tagged)-Human ER lipid raft associated 2 (ERLIN2), transcript variant 2
ORF Plasmid: tGFP
"NM_001003790" in other vectors (4)
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CNY 1,200.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C8orf2; Erlin-2; NET32; SPFH2; SPG18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221757 representing NM_001003790
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTGGGAGCAGTTGTGGCTGTGGCTTCCAGTTTCTTTTGTGCATCTCTCTTCTCAGCTGTGC ACAAGATAGAAGAGGGACATATTGGGGTATATTACAGAGGCGGTGCCCTGCTGACTTCGACCAGCGGCCC TGGTTTCCATCTCATGCTCCCTTTCATCACATCATATAAGTCTGTGCAGACCACACTCCAGACAGATGAG GTGAAGAATGTACCTTGTGGGACTAGTGGTGGTGTGATGATCTACTTTGACAGAATTGAAGTGGTGAACT TCCTGGTCCCGAACGCAGTGTATGATATAGTGAAGAACTATACTGCTGACTATGACAAGGCCCTCATCTT CAACAAGATCCACCACGAACTGAACCAGTTCTGCAGTGTGCACACGCTTCAAGAGGTCTACATTGAGCTG TTTGGACTGGAAAATGATTTTTCCCAGGAATCTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221757 representing NM_001003790
Red=Cloning site Green=Tags(s) MAQLGAVVAVASSFFCASLFSAVHKIEEGHIGVYYRGGALLTSTSGPGFHLMLPFITSYKSVQTTLQTDE VKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIEL FGLENDFSQESS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001003790 |
| ORF Size | 456 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001003790.4 |
| RefSeq Size | 1744 bp |
| RefSeq ORF | 459 bp |
| Locus ID | 11160 |
| UniProt ID | O94905 |
| MW | 16.6 kDa |
| Gene Summary | This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221757L3 | Lenti ORF clone of Human ER lipid raft associated 2 (ERLIN2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221757L4 | Lenti ORF clone of Human ER lipid raft associated 2 (ERLIN2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG221757 | ERLIN2 (tGFP-tagged) - Human ER lipid raft associated 2 (ERLIN2), transcript variant 2 |
CNY 4,370.00 |
|
| SC300544 | ERLIN2 (untagged)-Human ER lipid raft associated 2 (ERLIN2), transcript variant 2 |
CNY 1,200.00 |
