CACNA1E (NM_000721) Human Tagged ORF Clone
CAT#: RC221733
- TrueORF®
CACNA1E (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant 3
ORF Plasmid: tGFP
"NM_000721" in other vectors (3)
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CNY 22,480.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BII; CACH6; CACNL1A6; Cav2.3; DEE69; EIEE69; gm139 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000721 |
ORF Size | 6810 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000721.4 |
RefSeq Size | 9734 bp |
RefSeq ORF | 6813 bp |
Locus ID | 777 |
UniProt ID | Q15878 |
Domains | ion_trans |
Protein Families | Druggable Genome, Ion Channels: Calcium, Transmembrane |
Protein Pathways | Calcium signaling pathway, MAPK signaling pathway, Type II diabetes mellitus |
MW | 256.9 kDa |
Gene Summary | Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1, alpha-2, beta, and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels, which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221733L1 | Lenti ORF clone of Human calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant 3, Myc-DDK-tagged |
CNY 24,880.00 |
|
RG221733 | CACNA1E (tGFP-tagged) - Human calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant 3 |
CNY 20,900.00 |
|
SC309052 | CACNA1E (untagged)-Human calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant 3 |
CNY 43,700.00 |
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