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PKC delta (PRKCD) (NM_006254) Human Tagged ORF Clone
CAT＃: RC221652
PRKCD (Myc-DDK-tagged)-Human protein kinase C, delta (PRKCD), transcript variant 1
ORF Plasmid: tGFP
"NM_006254" in other vectors (7)
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CNY 7,424.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALPS3; CVID9; MAY1; nPKC-delta; PKCD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221652 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGTTCCTGCGCATCGCCTTCAACTCCTATGAGCTGGGCTCCCTGCAGGCCGAGGACGAGGCGA ACCAGCCCTTCTGTGCCGTGAAGATGAAGGAGGCGCTCAGCACAGAGCGTGGGAAAACACTGGTGCAGAA GAAGCCGACCATGTATCCTGAGTGGAAGTCGACGTTCGACGCCCACATCTATGAGGGGCGCGTCATCCAG ATTGTGCTAATGCGGGCAGCAGAGGAGCCAGTGTCTGAGGTGACCGTGGGTGTGTCGGTGCTGGCCGAGC GCTGCAAGAAGAACAATGGCAAGGCTGAGTTCTGGCTGGACCTGCAGCCTCAGGCCAAGGTGTTGATGTC TGTTCAGTATTTCCTGGAGGACGTGGATTGCAAACAGTCTATGCGCAGTGAGGACGAGGCCAAGTTCCCA ACGATGAACCGCCGCGGAGCCATCAAACAGGCCAAAATCCACTACATCAAGAACCATGAGTTTATCGCCA CCTTCTTTGGGCAACCCACCTTCTGTTCTGTGTGCAAAGACTTTGTCTGGGGCCTCAACAAGCAAGGCTA CAAATGCAGGCAATGTAACGCTGCCATCCACAAGAAATGCATCGACAAGATCATCGGCAGATGCACTGGC ACCGCGGCCAACAGCCGGGACACTATATTCCAGAAAGAACGCTTCAACATCGACATGCCGCACCGCTTCA AGGTTCACAACTACATGAGCCCCACCTTCTGTGACCACTGCGGCAGCCTGCTCTGGGGACTGGTGAAGCA GGGATTAAAGTGTGAAGACTGCGGCATGAATGTGCACCATAAATGCCGGGAGAAGGTGGCCAACCTCTGC GGCATCAACCAGAAGCTTTTGGCTGAGGCCTTGAACCAAGTCACCCAGAGAGCCTCCCGGAGATCAGACT CAGCCTCCTCAGAGCCTGTTGGGATATATCAGGGTTTCGAGAAGAAGACCGGAGTTGCTGGGGAGGACAT GCAAGACAACAGTGGGACCTACGGCAAGATCTGGGAGGGCAGCAGCAAGTGCAACATCAACAACTTCATC TTCCACAAGGTCCTGGGCAAAGGCAGCTTCGGGAAGGTGCTGCTTGGAGAGCTGAAGGGCAGAGGAGAGT ACTTTGCCATCAAGGCCCTCAAGAAGGATGTGGTCCTGATCGACGACGACGTGGAGTGCACCATGGTTGA GAAGCGGGTGCTGACACTTGCCGCAGAGAATCCCTTTCTCACCCACCTCATCTGCACCTTCCAGACCAAG GACCACCTGTTCTTTGTGATGGAGTTCCTCAACGGGGGGGACCTGATGTACCACATCCAGGACAAAGGCC GCTTTGAACTCTACCGTGCCACGTTTTATGCCGCTGAGATAATGTGTGGACTGCAGTTTCTACACAGCAA GGGCATCATTTACAGGGACCTCAAACTGGACAATGTGCTGTTGGACCGGGATGGCCACATCAAGATTGCC GACTTTGGGATGTGCAAAGAGAACATATTCGGGGAGAGCCGGGCCAGCACCTTCTGCGGCACCCCTGACT ATATCGCCCCTGAGATCCTACAGGGCCTGAAGTACACATTCTCTGTGGACTGGTGGTCTTTCGGGGTCCT TCTGTACGAGATGCTCATTGGCCAGTCCCCCTTCCATGGTGATGATGAGGATGAACTCTTCGAGTCCATC CGTGTGGACACGCCACATTATCCCCGCTGGATCACCAAGGAGTCCAAGGACATCCTGGAGAAGCTCTTTG AAAGGGAACCAACCAAGAGGCTGGGAGTGACGGGAAACATCAAAATCCACCCCTTCTTCAAGACCATAAA CTGGACTCTGCTGGAAAAGCGGAGGTTGGAGCCACCCTTCAGGCCCAAAGTGAAGTCACCCAGAGACTAC AGTAACTTTGACCAGGAGTTCCTGAACGAGAAGGCGCGCCTCTCCTACAGCGACAAGAACCTCATCGACT CCATGGACCAGTCTGCATTCGCTGGCTTCTCCTTTGTGAACCCCAAATTCGAGCACCTCCTGGAAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221652 protein sequence
Red=Cloning site Green=Tags(s) MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQ IVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFP TMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTG TAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLC GINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFI FHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTK DHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIA DFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESI RVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDY SNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006254 |
| ORF Size | 2028 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006254.2 |
| RefSeq Size | 2850 bp |
| RefSeq ORF | 2031 bp |
| Locus ID | 5580 |
| UniProt ID | Q05655 |
| Domains | pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, GnRH signaling pathway, Neurotrophin signaling pathway, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction |
| MW | 77.5 kDa |
| Gene Summary | The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Chronic Granulomatous Disease-Like Presentation of a Child with Autosomal Recessive PKCδ Deficiency
,null,
Journal of Clinical Immunology
,PubMed ID 35585372
[PKC delta]
|
|
Impaired respiratory burst contributes to infections in PKCδ-deficient patients
,null,
The Journal of Experimental Medicine
,PubMed ID 34264265
[PKC delta]
|
|
Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer
,Lee, PC;Fang, YF;Yamaguchi, H;Wang, WJ;Chen, TC;Hong, X;Ke, B;Xia, W;Wei, Y;Zha, Z;Wang, Y;Kuo, HP;Wang, CW;Tu, CY;Chen, CH;Huang, WC;Chiang, SF;Nie, L;Hou, J;Chen, CT;Huo, L;Yang, WH;Deng, R;Nakai, K;Hsu, YH;Chang, SS;Chiu, TJ;Tang, J;Zhang, R;Wang, L;Fang, B;Chen, T;Wong, KK;Hsu, JL;Hung, MC;,
Cancer Cell
,PubMed ID 30537515
[PKC delta]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221652L1 | Lenti ORF clone of Human protein kinase C, delta (PRKCD), transcript variant 1, Myc-DDK-tagged |
CNY 9,824.00 |
|
| RC221652L2 | Lenti ORF clone of Human protein kinase C, delta (PRKCD), transcript variant 1, mGFP tagged |
CNY 9,824.00 |
|
| RC221652L3 | Lenti ORF clone of Human protein kinase C, delta (PRKCD), transcript variant 1, Myc-DDK-tagged |
CNY 9,824.00 |
|
| RC221652L4 | Lenti ORF clone of Human protein kinase C, delta (PRKCD), transcript variant 1, mGFP tagged |
CNY 9,824.00 |
|
| RG221652 | PRKCD (tGFP-tagged) - Human protein kinase C, delta (PRKCD), transcript variant 1 |
CNY 9,024.00 |
|
| SC303761 | PRKCD (untagged)-Human protein kinase C, delta (PRKCD), transcript variant 1 |
CNY 7,220.00 |
|
| SC323442 | PRKCD (untagged)-Kinase deficient mutant (K378M) of Human protein kinase C, delta (PRKCD), transcript variant 1 |
CNY 7,440.00 |
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