SOAT 2 (SOAT2) (NM_003578) Human Tagged ORF Clone
CAT#: RC221499
SOAT2 (Myc-DDK-tagged)-Human sterol O-acyltransferase 2 (SOAT2)
ORF Plasmid: tGFP
"NM_003578" in other vectors (4)
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CNY 3,888.00
CNY 4,560.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ACACT2; ACAT2; ARGP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221499 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAGGCGGGGCCCGTCTGCGTCTGCAGAGGACAGGAGGGCTGGGAGGGGAGCGGGAGCGCCAAC CCTGTGGAGATGGAAACACTGAGACGCACAGAGCCCCGGACTTGGTACAATGGACCCGACACATGGAGGC TGTGAAGGCACAATTGCTGGAGCAAGCGCAGGGACAACTGAGGGAGCTGCTGGATCGGGCCATGCGGGAG GCTATACAATCCTACCCATCACAAGACAAACCTCTGCCCCCACCTCCCCCAGGTTCCTTGAGCAGGACCC AGGAGCCATCCCTGGGGAAACAGAAAGTTTTCATCATCCGCAAGTCCCTGCTTGATGAGCTGATGGAGGT GCAGCATTTCCGCACCATCTACCACATGTTCATCGCTGGCCTGTGTGTCTTCATCATCAGCACCCTGGCC ATCGACTTCATTGATGAGGGCAGGCTGCTGCTGGAGTTTGACCTACTGATCTTCAGCTTCGGACAGCTGC CATTGGCGCTGGTGACCTGGGTGCCCATGTTTCTGTCCACCCTGTTGGCGCCGTACCAGGCCCTACGGCT GTGGGCCAGGGGCACCTGGACGCAGGCGACGGGCCTGGGCTGTGCGCTTTTAGCCGCCCACGCCGTGGTG CTCTGCGCGCTGCCGGTCCACGTGGCCGTGGAGCATCAGCTCCCGCCGGCCTCCCGTTGTGTCCTGGTCT TCGAGCAGGTTAGGTTCCTGATGAAAAGCTACTCCTTCCTGAGAGAGGCTGTGCCTGGGATCCTTCGTGC CAGACGAGGTGAGGGGATCCAGGCCCCCAGTTTCTCCAGCTACCTCTACTTCCTCTTCTGCCCAACACTC ATCTACAGGGAGACTTACCCTAGGACGCCCTATGTCAGGTGGAATTATGTGGCCAAGAACTTTGCCCAGG CCCTGGGATGTGTGCTCTATGCCTGCTTCATCCTGGGCCGCCTCTGTGTTCCTGTCTTTGCCAACATGAG CCGAGAGCCCTTCAGCACCCGTGCCCTGGTGCTCTCTATCCTGCATGCCACGTTGCCAGGCATCTTCATG CTGCTGCTCATCTTCTTTGCCTTCCTCCATTGCTGGCTCAACGCCTTTGCCGAGATGCTACGATTTGGAG ACAGGATGTTCTACCGGGACTGGTGGAACTCAACGTCCTTCTCCAACTACTACCGCACTTGGAACGTGGT GGTCCATGACTGGCTGTACAGCTACGTGTATCAGGATGGGCTGCGGCTCCTTGGTGCCCGGGCCCGAGGG GTAGCCATGCTGGGTGTGTTCCTGGTCTCCGCAGTGGCCCATGAGTATATCTTCTGCTTCGTCCTGGGGT TCTTCTATCCCGTCATGCTGATACTCTTCCTTGTCATTGGAGGAATGTTGAACTTCATGATGCATGACCA GCGCACCGGCCCGGCATGGAACGTGCTGATGTGGACCATGCTGTTTCTAGGCCAGGGAATCCAGGTCAGC CTGTACTGCCAGGAGTGGTACGCACGGCGGCACTGCCCCTTACCCCAGGCAACTTTCTGGGGGCTGGTGA CACCTCGATCTTGGTCCTGCCATACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221499 protein sequence
Red=Cloning site Green=Tags(s) MEPGGARLRLQRTGGLGGERERQPCGDGNTETHRAPDLVQWTRHMEAVKAQLLEQAQGQLRELLDRAMRE AIQSYPSQDKPLPPPPPGSLSRTQEPSLGKQKVFIIRKSLLDELMEVQHFRTIYHMFIAGLCVFIISTLA IDFIDEGRLLLEFDLLIFSFGQLPLALVTWVPMFLSTLLAPYQALRLWARGTWTQATGLGCALLAAHAVV LCALPVHVAVEHQLPPASRCVLVFEQVRFLMKSYSFLREAVPGILRARRGEGIQAPSFSSYLYFLFCPTL IYRETYPRTPYVRWNYVAKNFAQALGCVLYACFILGRLCVPVFANMSREPFSTRALVLSILHATLPGIFM LLLIFFAFLHCWLNAFAEMLRFGDRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLRLLGARARG VAMLGVFLVSAVAHEYIFCFVLGFFYPVMLILFLVIGGMLNFMMHDQRTGPAWNVLMWTMLFLGQGIQVS LYCQEWYARRHCPLPQATFWGLVTPRSWSCHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003578 |
ORF Size | 1566 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003578.4 |
RefSeq Size | 2073 bp |
RefSeq ORF | 1569 bp |
Locus ID | 8435 |
UniProt ID | O75908 |
Protein Families | Transmembrane |
Protein Pathways | Steroid biosynthesis |
MW | 59.8 kDa |
Gene Summary | Summary:This gene is a member of a small family of acyl coenzyme A:cholesterol acyltransferases. The gene encodes a membrane-bound enzyme localized in the endoplasmic reticulum that produces intracellular cholesterol esters from long-chain fatty acyl CoA and cholesterol. The cholesterol esters are then stored as cytoplasmic lipid droplets inside the cell. The enzyme is implicated in cholesterol absorption in the intestine and in the assembly and secretion of apolipoprotein B-containing lipoproteins such as very low density lipoprotein (VLDL). Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Mitotane inhibits Sterol-O-Acyl Transferase 1 triggering lipid-mediated endoplasmic reticulum stress and apoptosis in adrenocortical carcinoma cells
,Sbiera, S;Leich, E;Liebisch, G;Sbiera, I;Schirbel, A;Wiemer, L;Matysik, S;Eckhardt, C;Gardill, F;Gehl, A;Kendl, S;Weigand, I;Bala, M;Ronchi, CL;Deutschbein, T;Schmitz, G;Rosenwald, A;Allolio, B;Fassnacht, M;Kroiss, M;,
Endocrinology
,PubMed ID 26305886
[SOAT 2]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221499L3 | Lenti ORF clone of Human sterol O-acyltransferase 2 (SOAT2), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC221499L4 | Lenti ORF clone of Human sterol O-acyltransferase 2 (SOAT2), mGFP tagged |
CNY 6,460.00 |
|
RG221499 | SOAT2 (tGFP-tagged) - Human sterol O-acyltransferase 2 (SOAT2) |
CNY 5,488.00 |
|
SC303352 | SOAT2 (untagged)-Human sterol O-acyltransferase 2 (SOAT2) |
CNY 3,896.00 |