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AMBN (NM_016519) Human Tagged ORF Clone
CAT＃: RC221219
- TrueORF®
AMBN (Myc-DDK-tagged)-Human ameloblastin (enamel matrix protein) (AMBN)
ORF Plasmid: tGFP
"NM_016519" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI1F |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221219 representing NM_016519
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCATCTAAGATTCCACTTTTCAAAATGAAGGACCTGATACTGATCCTATGCCTCCTGGAAATGA GTTTTGCAGTGCCGTTCTTTCCTCAGCAATCTGGAACACCGGGTATGGCTAGTTTGAGCCTTGAGACAAT GAGACAGTTGGGAAGTCTGCAGAGATTAAACACACTTTCTCAGTATTCTAGATACGGCTTTGGAAAATCA TTTAATTCTTTGTGGATGCACGGTCTCCTCCCACCACATTCCTCTCTTCCATGGATGAGGCCAAGAGAAC ATGAAACTCAACAGTATGAATATTCTTTGCCTGTGCATCCCCCACCTCTCCCATCACAGCCATCCTTGAA GCCTCAACAGCCAGGACTGAAACCTTTTCTCCAGTCTGCTGCTGCAACCACCAACCAGGCCACAGCACTG AAAGAAGCACTTCAGCCTCCAATTCACCTGGGACATCTGCCCTTGCAGGAAGGAGAACTGCCTCTGGTTC AGCAGCAGGTGGCACCATCAGATAAGCCACCAAAGCCTGAGCTCCCAGGAGTAGATTTTGCTGATCCACA AGGTCCATCACTCCCAGGAATGGATTTTCCTGATCCACAAGGTCCATCACTCCCAGGATTGGATTTTGCT GATCCACAAGGTTCAACAATTTTCCAAATAGCCCGTTTGATTTCTCACGGACCAATGCCACAAAATAAAC AATCTCCACTTTATCCAGGAATGTTGTACGTGCCTTTTGGAGCAAATCAATTGAATGCCCCTGCCAGACT TGGCATCATGAGTTCAGAAGAAGTGGCAGGCGGGAGAGAAGACCCAATGGCCTATGGAGCCATGTTTCCA GGATTTGGAGGCATGAGGCCCGGCTTTGAGGGAATGCCCCACAACCCAGCTATGGGCGGTGACTTCACTC TGGAATTTGACTCCCCAGTGGCTGCCACCAAAGGCCCTGAGAACGAAGAAGGAGGTGCACAAGGCTCCCC TATGCCGGAGGCCAACCCAGACAATCTAGAAAACCCAGCTTTCCTTACAGAGCTAGAACCTGCTCCCCAC GCAGGGCTCCTTGCTCTCCCTAAGGATGACATTCCCGGCCTGCCAAGGAGCCCTTCAGGGAAGATGAAGG GACTCCCCAGCGTCACCCCAGCAGCTGCTGACCCACTGATGACCCCTGAATTAGCTGATGTTTATAGGAC CTACGATGCTGACATGACCACATCCGTGGATTTCCAGGAAGAAGCAACCATGGATACCACGATGGCCCCA AACTCTCTGCAAACATCCATGCCAGGAAACAAAGCCCAGGAGCCCGAGATGATGCATGACGCATGGCATT TCCAAGAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221219 representing NM_016519
Red=Cloning site Green=Tags(s) MSASKIPLFKMKDLILILCLLEMSFAVPFFPQQSGTPGMASLSLETMRQLGSLQRLNTLSQYSRYGFGKS FNSLWMHGLLPPHSSLPWMRPREHETQQYEYSLPVHPPPLPSQPSLKPQQPGLKPFLQSAAATTNQATAL KEALQPPIHLGHLPLQEGELPLVQQQVAPSDKPPKPELPGVDFADPQGPSLPGMDFPDPQGPSLPGLDFA DPQGSTIFQIARLISHGPMPQNKQSPLYPGMLYVPFGANQLNAPARLGIMSSEEVAGGREDPMAYGAMFP GFGGMRPGFEGMPHNPAMGGDFTLEFDSPVAATKGPENEEGGAQGSPMPEANPDNLENPAFLTELEPAPH AGLLALPKDDIPGLPRSPSGKMKGLPSVTPAAADPLMTPELADVYRTYDADMTTSVDFQEEATMDTTMAP NSLQTSMPGNKAQEPEMMHDAWHFQEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016519 |
| ORF Size | 1341 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016519.6 |
| RefSeq Size | 2025 bp |
| RefSeq ORF | 1344 bp |
| Locus ID | 258 |
| UniProt ID | Q9NP70 |
| Protein Families | Secreted Protein |
| MW | 48.3 kDa |
| Gene Summary | This gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix formation and mineralization. This gene is located in the calcium-binding phosphoprotein gene cluster on chromosome 4. Mutations in this gene may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221219L3 | Lenti-ORF clone of AMBN (Myc-DDK-tagged)-Human ameloblastin (enamel matrix protein) (AMBN) |
CNY 5,890.00 |
|
| RC221219L4 | Lenti-ORF clone of AMBN (mGFP-tagged)-Human ameloblastin (enamel matrix protein) (AMBN) |
CNY 5,890.00 |
|
| RG221219 | AMBN (tGFP-tagged) - Human ameloblastin (enamel matrix protein) (AMBN) |
CNY 4,370.00 |
|
| SC304427 | AMBN (untagged)-Human ameloblastin (enamel matrix protein) (AMBN) |
CNY 3,656.00 |
