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SORBS2 (NM_021069) Human Tagged ORF Clone
CAT＃: RC221118
SORBS2 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2
ORF Plasmid: tGFP
"NM_021069" in other vectors (4)
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CNY 7,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARGBP2; PRO0618 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221118 representing NM_021069
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTACTATCAGAGGCCGTTTTCCCCCTCGGCATATTCTCTCCCAGCCTCACTCAACTCCAGCATTG TCATGCAGCACGGCACATCCCTCGATTCCACAGACACATATCCCCAGCATGCGCAGTCTCTGGATGGCAC CACCAGCAGCTCTATCCCCCTGTACCGATCCTCAGAGGAAGAGAAGAGAGTGACAGTCATCAAAGCCCCG CATTACCCAGGGATCGGGCCCGTGGATGAATCCGGAATCCCCACAGCAATTAGAACGACAGTCGACCGGC CCAAGGACTGGTACAAGACGATGTTTAAGCAAATTCACATGGTGCACAAGCCGGATGATGACACAGACAT GTATAATACTCCTTATACATACAATGCAGGTCTGTACAACCCACCCTACAGTGCTCAGTCACACCCTGCT GCAAAGACCCAAACCTACAGACCTCTTTCCAAAAGCCACTCCGACAACAGCCCCAATGCCTTTAAGGATG CGTCCTCCCCAGTGCCTCCCCCACATGTTCCACCTCCAGTCCCGCCGCTTCGACCAAGAGATCGGTCTTC AACAGAAAAGCATGACTGGGATCCTCCAGACAGAAAAGTGGACACAAGAAAATTTCGGTCTGAGCCAAGG AGTATTTTTGAATATGAACCTGGCAAGTCATCAATTCTTCAGCATGAAAGACCAGCCTCCTTGTATCAGT CCTCTATAGACAGAAGCCTGGAAAGACCCATGAGTTCTGCAAGCATGGCCAGTGACTTCAGGAAGCGGAG GAAGAGCGAGCCTGCAGTGGGTCCACCACGGGGCTTGGGAGATCAAAGTGCGAGCAGGACTAGCCCAGGC CGAGTGGACCTCCCAGGATCAAGCACCACTCTTACAAAGTCTTTCACTAGCTCTTCTCCTTCTTCCCCAT CAAGAGCAAAAGGTGGGGATGATAGCAAAATATGTCCATCCCTTTGCAGTTACTCAGGGCTCAACGGCAA CCCCTCCAGTGAGTTAGATTACTGTAGCACTTACAGACAGCACTTGGATGTCCCTCGGGACTCACCAAGA GCCATTAGTTTCAAGAACGGCTGGCAAATGGCCCGGCAAAACGCAGAAATCTGGAGCAGCACGGAAGAAA CCGTCTCTCCCAAAATCAAATCCCGGAGCTGTGACGATCTCCTAAACGACGACTGCGATAGCTTCCCGGA CCCGAAAGTCAAGTCGGAAAGCATGGGCTCCCTGTTATGTGAGGAGGACTCCAAGGAGAGCTGCCCCATG GCGTGGGGGTCCCCCTACGTCCCGGAGGTTCGCAGTAACGGCAGATCAAGGATCAGACACAGGTCAGCCC GCAACGCCCCGGGGTTCCTGAAGATGTACAAGAAAATGCACCGCATCAACCGCAAGGACCTGATGAACTC CGAGGTGATCTGCTCCGTGAAGTCCAGGATTCTGCAGTACGAGAGCGAGCAGCAGCACAAGGACCTGCTG CGCGCCTGGAGCCAGTGCTCCACGGAGGAGGTGCCCCGGGACATGGTGCCCACACGCATCTCCGAATTCG AAAAGCTGATCCAGAAGTCCAAATCCATGCCAAATTTAGGGGATGACATGCTGTCTCCCGTAACCCTAGA ACCACCGCAAAATGGCCTATGTCCCAAGAGGAGGTTTTCCATTGAGTATTTGCTGGAGGAAGAAAATCAA AGCGGCCCCCCCGCTCGGGGCCGGCGAGGCTGCCAGTCTAACGCCCTGGTGCCCATTCACATTGAAGTCA CCAGCGATGAGCAGCCCCGAGCACACGTGGAGTTTTCCGACAGCGACCAGGACGGGGTTGTGTCCGACCA CAGTGACTACATTCACCTAGAGGGGTCATCCTTCTGCAGTGAAAGTGACTTTGATCACTTTTCCTTCACA TCCTCCGAAAGCTTTTACGGATCCAGCCACCACCACCACCATCACCACCACCACCACCACCGCCACCTCA TCAGCTCCTGCAAAGGCAGGTGCCCGGCCTCCTACACTCGATTTACCACAATGTTAAAACACGAAAGAGC CAGACACGAAAACACCGAGGAGCCCAGAAGGCAAGAAATGGACCCTGGCCTTTCTAAACTTGCTTTTCTA GTCAGTCCTGTGCCTTTCCGGAGGAAAAAAAATTCGGCTCCTAAGAAACAGACTGAAAAGGCAAAATGTA AAGCATCTGTGTTTGAGGCTCTGGACTCTGCCCTTAAAGACATCTGTGACCAAATTAAAGCTGAAAAGAA AAGGGGGAGCTTGCCGGACAACAGCATCCTGCACCGCCTCATCAGTGAGCTGCTGCCAGATGTTCCCGAG AGGAACTCATCCCTGAGAGCGCTGAGGAGGAGCCCCCTGCACCAGCCTCTCCACCCACTGCCTCCCGATG GTGCTATTCATTGTCCACCCTACCAGAATGACTGCGGGAGAATGCCCCGCAGTGCCTCTTTCCAAGACGT GGACACAGCCAACAGCAGCTGCCACCACCAAGACCGTGGCGGTGCACTCCAAGACCGTGAGTCCCCTAGA AGTTACTCATCCACTTTGACTGACATGGGGAGAAGTGCACCAAGGGAAAGAAGAGGAACTCCAGAAAAAG AGAAATTGCCTGCAAAAGCTGTTTATGATTTTAAGGCTCAGACATCTAAGGAGTTGTCATTTAAGAAAGG AGATACTGTCTACATCCTCAGGAAAATTGATCAAAATTGGTATGAGGGAGAACACCACGGGAGAGTGGGC ATCTTCCCGATCTCATACGTAGAGAAACTCACACCTCCTGAGAAAGCACAGCCTGCAAGACCACCTCCGC CAGCCCAGCCCGGAGAAATCGGAGAAGCTATAGCCAAATACAACTTCAACGCAGACACAAATGTGGAGCT GTCACTGAGAAAGGGAGATAGAGTTATTCTTCTTAAAAGAGTTGATCAAAACTGGTATGAAGGTAAAATC CCAGGAACCAACAGACAAGGCATCTTCCCTGTTTCCTATGTGGAGGTCGTCAAGAAGAACACAAAAGGTG CTGAGGACTACCCTGACCCTCCAATACCCCACAGCTATTCTAGTGATAGGATTCACAGCTTGAGCTCAAA TAAGCCACAGCGTCCTGTGTTTACTCATGAAAATATTCAAGGTGGGGGGGAACCGTTTCAGGCTCTGTAT AACTATACTCCCAGGAATGAAGATGAGCTGGAGCTCAGAGAAAGTGATGTCATTGATGTCATGGAAAAGT GTGATGACGGCTGGTTTGTGGGGACCTCAAGAAGAACCAAATTCTTTGGTACTTTCCCCGGAAACTACGT CAAGAGGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221118 representing NM_021069
Red=Cloning site Green=Tags(s) MSYYQRPFSPSAYSLPASLNSSIVMQHGTSLDSTDTYPQHAQSLDGTTSSSIPLYRSSEEEKRVTVIKAP HYPGIGPVDESGIPTAIRTTVDRPKDWYKTMFKQIHMVHKPDDDTDMYNTPYTYNAGLYNPPYSAQSHPA AKTQTYRPLSKSHSDNSPNAFKDASSPVPPPHVPPPVPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPR SIFEYEPGKSSILQHERPASLYQSSIDRSLERPMSSASMASDFRKRRKSEPAVGPPRGLGDQSASRTSPG RVDLPGSSTTLTKSFTSSSPSSPSRAKGGDDSKICPSLCSYSGLNGNPSSELDYCSTYRQHLDVPRDSPR AISFKNGWQMARQNAEIWSSTEETVSPKIKSRSCDDLLNDDCDSFPDPKVKSESMGSLLCEEDSKESCPM AWGSPYVPEVRSNGRSRIRHRSARNAPGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYESEQQHKDLL RAWSQCSTEEVPRDMVPTRISEFEKLIQKSKSMPNLGDDMLSPVTLEPPQNGLCPKRRFSIEYLLEEENQ SGPPARGRRGCQSNALVPIHIEVTSDEQPRAHVEFSDSDQDGVVSDHSDYIHLEGSSFCSESDFDHFSFT SSESFYGSSHHHHHHHHHHHRHLISSCKGRCPASYTRFTTMLKHERARHENTEEPRRQEMDPGLSKLAFL VSPVPFRRKKNSAPKKQTEKAKCKASVFEALDSALKDICDQIKAEKKRGSLPDNSILHRLISELLPDVPE RNSSLRALRRSPLHQPLHPLPPDGAIHCPPYQNDCGRMPRSASFQDVDTANSSCHHQDRGGALQDRESPR SYSSTLTDMGRSAPRERRGTPEKEKLPAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVG IFPISYVEKLTPPEKAQPARPPPPAQPGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKI PGTNRQGIFPVSYVEVVKKNTKGAEDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALY NYTPRNEDELELRESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021069 |
| ORF Size | 3300 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021069.5 |
| RefSeq Size | 4435 bp |
| RefSeq ORF | 3303 bp |
| Locus ID | 8470 |
| UniProt ID | O94875 |
| Domains | SH3, Sorb |
| MW | 123.9 kDa |
| Gene Summary | Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. This gene encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221118L3 | Lenti ORF clone of Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2, Myc-DDK-tagged |
CNY 13,490.00 |
|
| RC221118L4 | Lenti ORF clone of Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2, mGFP tagged |
CNY 13,490.00 |
|
| RG221118 | SORBS2 (tGFP-tagged) - Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2 |
CNY 12,730.00 |
|
| SC315181 | SORBS2 (untagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2 |
CNY 21,280.00 |
