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LIMCH1 (NM_014988) Human Tagged ORF Clone
CAT＃: RC221099
LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1
ORF Plasmid: tGFP
"NM_014988" in other vectors (4)
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CNY 7,544.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LIMCH1A; LMO7B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221099 representing NM_014988
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGTCCCGCTCTCGGTCTGGAAGCTCTTCAGCCCCTGCAGCCCGAGCCGCCCCCCGAGCCCGCCT TCTCCGAGGCGCAGAAGTGGATTGAGCAAGTAACTGGCAGAAGTTTTGGTGATAAAGATTTTCGGACAGG TTTAGAAAATGGAATCCTCCTCTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAAT AGATTGCCTACCCCCATTGCAGGACTGGACAATATTATCTTATTCTTGAGAGGTTGTAAAGAGCTCGGCC TTAAAGAATCTCAACTTTTTGACCCGAGTGACCTCCAGGATACATCCAACAGAGTAACAGTCAAGAGCCT TGATTATAGTAGGAAGCTGAAAAATGTATTAGTTACCATTTACTGGCTGGGAAAAGCAGCAAACAGCTGC ACATCCTACAGCGGAACGACACTAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGAAAGGACA CTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCTGGGATTCCGAGCG CAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGATTCCTTTGGCTCT CGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGAGGAAGCGACTCTG AATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGGCGGACTTCCCA TGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCAGACGGCCTACGTC CCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGTACCGCCACCTCCC CGCTGGGTGGGGAGAGGCCCTTCAGATACGGTCCGAGAACTCCTGTGTCTGATGACGCAGAGAGCACCAG CATGTTTGACATGCGGTGTGAGGAGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTG CAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTC GTAGAAGAAGTGTTTCTCAGGACTTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGC TGGAGAAGATGGGACAAGTGAACGAAGGAAAAGCATCAAAACCTACAGAGAAATTGTTCAAGAAAAAGAG CGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAACGCTCGGTCCCAGGAGGAGGCAGAGGGGATCCTTC AACAGTACATTGAGAGGTTCACCATCAGTGAGGCTGTTCTCGAACGCTTGGAGATGCCAAAAATTCTGGA AAGAAGCCATTCAACAGAGCCAAATTTATCCTCCTTCCTGAATGACCCCAATCCCATGAAATACCTGCGG CAACAGTCACTGCCTCCACCCAAATTCACTGCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGG ATACCAGCATGTCAGCAGGCAGTGGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCTATGCTGAC ACCCAAGCCTTACTCCCAGCCCAAAAATTCTCAAGATGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTC AGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAGAAGGAAAGAGAGTGTCCCACGGTGGCACCTGCCC ACTCCTTAACCAAATCCCAGATGTTTGAAGGTGTGGCCAGAGTGCACGGGTCTCCACTGGAGCTGAAACA AGACAACGGTAGCATCGAGATCAACATAAAGAAGCCAAACTCTGTTCCCCAAGAGCTCGCAGCAACCACT GAGAAAACGGAACCGAATAGTCAAGAGGACAAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAAC TTGCCTCATCAGAACCACAGCATTTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCCTTTGTGGA ATTTCCCTCCAGCCCCCAGCTGAAGAATGATGTGTCGGAAGAAAAAGACCAGAAGAAACCAGAAAATGAA ATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTGGTAAAGCCAAAATCTCCAGAACCCGAAGCAA CGCTGACATTTCCATTTCTGGACAAAATGCCTGAAGCCAACCAACTACATTTGCCAAATCTCAATTCTCA AGTGGATTCTCCAAGCAGTGAGAAGTCACCTGTTACGACACCTCAGTTTAAGTTCTGGGCATGGGACCCA GAAGAGGAGCGCAGGCGACAGGAAAAATGGCAACAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGA AGGAGCAGGACAAGCTGAAAGAAGAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATA CTATGAGGAGGAGCGTAAGATAATTGAAGACACTGTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGAC CAGCTGTCTACCTCTTCCTCCATGACTGAAGGCAGTGGGACAATGAATAAGATAGACCTGGGAAACTGTC AAGATGAAAAACAAGACAGAAGATGGAAGAAATCATTCCAGGGAGATGACAGTGACTTATTGCTGAAGAC TAGGGAAAGTGATCGACTGGAGGAGAAGGGCAGCCTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCT GTATCAAAAGGAGTCCATGAAGACCATCAGCTGGATACCGAGGCTGGGGCCCCACACTGTGGAACAAACC CACAGCTTGCTCAGGATCCATCCCAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAAGATGTGAA GCCAAAAACCCTCCCGCTGGATAAAAGCATTAACCATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGAAA AGTCCCCGAGAGCACTTCCAGGCTGGGCCTTTCTCTCCCTGTTCTCCCACCCCTCCCGGTCAGTCACCAA ACAGGTCTATAAGTGGAAAGAAGCTGTGCTCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATGAT CATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAGGTGTGGAATTTGTAAAGGCCAGCTTGGA GATGCAGTGAGTGGGACGGATGTTAGGATTCGAAATGGTCTCCTGAACTGTAATGATTGCTACATGCGAT CCAGAAGTGCCGGGCAGCCTACAACATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221099 representing NM_014988
Red=Cloning site Green=Tags(s) MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKIN RLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSC TSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGS RSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYV PAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQL QLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKE RRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLR QQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKV SVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATT EKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENE MSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDP EEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSAD QLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNP VSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKK SPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLG DAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014988 |
| ORF Size | 3249 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014988.1, NP_055803.1 |
| RefSeq Size | 5113 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 22998 |
| UniProt ID | Q9UPQ0 |
| MW | 121.7 kDa |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221099L3 | Lenti ORF clone of Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, Myc-DDK-tagged |
CNY 13,300.00 |
|
| RC221099L4 | Lenti ORF clone of Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mGFP tagged |
CNY 13,300.00 |
|
| RG221099 | LIMCH1 (tGFP-tagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1 |
CNY 12,540.00 |
|
| SC304197 | LIMCH1 (untagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1 |
CNY 7,552.00 |
