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NDRG4 (NM_020465) Human Tagged ORF Clone
CAT＃: RC221058
NDRG4 (Myc-DDK-tagged)-Human NDRG family member 4 (NDRG4), transcript variant 1
ORF Plasmid: tGFP
"NM_020465" in other vectors (6)
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CNY 3,656.00
CNY 4,370.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BDM1; SMAP-8; SMAP8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221058 representing NM_020465
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGCTGCAGGAGCTGCGATTCCCTGAGGAGAAGCCGCTGCTCCGGGGCCAGGACGCCACCGAGC TGGAGAGCTCCGATGCCTTCCTCTTGGCTGCAGACACAGACTGGAAGGAACATGACATCGAGACACCCTA CGGCCTTCTGCATGTAGTGATCCGGGGCTCCCCCAAGGGGAACCGCCCAGCCATCCTCACCTACCATGAT GTGGGCCTCAACCACAAACTATGCTTCAACACCTTCTTCAACTTCGAGGACATGCAGGAGATCACCAAGC ACTTTGTGGTGTGTCACGTGGATGCCCCTGGACAACAGGTGGGGGCGTCGCAGTTTCCTCAGGGGTACCA GTTCCCCTCCATGGAGCAGCTGGCTGCCATGCTCCCCAGCGTGGTGCAGCATTTCGGGTTCAAGTATGTG ATTGGCATCGGAGTGGGCGCCGGAGCCTATGTGCTGGCCAAGTTTGCACTCATCTTCCCCGACCTGGTGG AGGGGCTGGTGCTGGTGAACATCGACCCCAATGGCAAAGGCTGGATAGACTGGGCTGCCACCAAGCTCTC CGGCCTAACTAGCACTTTACCCGACACGGTGCTCTCCCACCTCTTCAGCCAGGAGGAGCTGGTGAACAAC ACAGAGTTGGTGCAGAGCTACCGGCAGCAGATTGGGAACGTGGTGAACCAGGCCAACCTGCAGCTCTTCT GGAACATGTACAACAGCCGCAGAGACCTGGACATTAACCGGCCTGGAACGGTGCCCAATGCCAAGACGCT CCGCTGCCCCGTGATGCTGGTGGTTGGGGATAATGCACCCGCTGAGGACGGGGTGGTGGAGTGCAACTCC AAACTGGACCCGACCACTACGACCTTCCTGAAGATGGCAGACTCTGGAGGGCTGCCCCAGGTCACACAGC CAGGGAAGCTGACTGAAGCCTTCAAATACTTCCTGCAAGGCATGGGCTACATGCCCTCAGCCAGCATGAC CCGCCTGGCACGCTCCCGCACTGCATCCCTCACCAGTGCCAGCTCGGTGGATGGCAGCCGCCCACAGGCC TGCACCCACTCGGAGAGCAGCGAGGGGCTGGGCCAGGTCAACCACACCATGGAGGTGTCCTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221058 representing NM_020465
Red=Cloning site Green=Tags(s) MAGLQELRFPEEKPLLRGQDATELESSDAFLLAADTDWKEHDIETPYGLLHVVIRGSPKGNRPAILTYHD VGLNHKLCFNTFFNFEDMQEITKHFVVCHVDAPGQQVGASQFPQGYQFPSMEQLAAMLPSVVQHFGFKYV IGIGVGAGAYVLAKFALIFPDLVEGLVLVNIDPNGKGWIDWAATKLSGLTSTLPDTVLSHLFSQEELVNN TELVQSYRQQIGNVVNQANLQLFWNMYNSRRDLDINRPGTVPNAKTLRCPVMLVVGDNAPAEDGVVECNS KLDPTTTTFLKMADSGGLPQVTQPGKLTEAFKYFLQGMGYMPSASMTRLARSRTASLTSASSVDGSRPQA CTHSESSEGLGQVNHTMEVSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020465 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020465.4 |
| RefSeq Size | 3288 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 65009 |
| UniProt ID | Q9ULP0 |
| Domains | Ndr |
| MW | 40.5 kDa |
| Gene Summary | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MiR-483-5p and miR-139-5p promote aggressiveness by targeting N-myc downstream-regulated gene family members in adrenocortical cancer
,Agosta, C;Laugier, J;Guyon, L;Denis, J;Bertherat, J;Libé, R;Boisson, B;Sturm, N;Feige, JJ;Chabre, O;Cherradi, N;,
Int. J. Cancer
,PubMed ID 29516499
[NDRG4]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221058L1 | Lenti ORF clone of Human NDRG family member 4 (NDRG4), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC221058L2 | Lenti ORF clone of Human NDRG family member 4 (NDRG4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC221058L3 | Lenti ORF clone of Human NDRG family member 4 (NDRG4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221058L4 | Lenti ORF clone of Human NDRG family member 4 (NDRG4), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG221058 | NDRG4 (tGFP-tagged) - Human NDRG family member 4 (NDRG4), transcript variant 1 |
CNY 5,256.00 |
|
| SC108447 | NDRG4 (untagged)-Human NDRG family member 4 (NDRG4), transcript variant 1 |
CNY 3,656.00 |
