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MTF2 (NM_007358) Human Tagged ORF Clone
CAT＃: RC221006
- TrueORF®
MTF2 (Myc-DDK-tagged)-Human metal response element binding transcription factor 2 (MTF2), transcript variant 1
ORF Plasmid: tGFP
"NM_007358" in other vectors (4)
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CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ976O13.2; M96; PCL2; TDRD19A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221006 representing NM_007358
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGACTCTACAGGGGCAGGTAATTCACTGGTCCACAAGCGGTCTCCTTTACGTCGAAACCAAAAGA CCCCAACATCCTTGACCAAGCTGTCTTTACAGGATGGACATAAAGCCAAAAAGCCAGCATGTAAATTTGA AGAGGGTCAGGATGTCCTAGCTAGATGGTCAGATGGCTTGTTTTATCTTGGCACTATCAAAAAGATAAAC ATATTGAAACAGAGCTGCTTCATCATATTTGAAGACAGTTCTAAATCCTGGGTTCTCTGGAAGGACATTC AAACAGGAGCCACTGGAAGTGGGGAAATGGTCTGTACAATATGTCAAGAAGAGTATTCAGAAGCTCCCAA TGAAATGGTTATATGTGACAAGTGTGGCCAAGGATATCATCAGTTGTGTCACACACCTCATATTGATTCC AGTGTGATTGATTCAGATGAAAAATGGCTCTGTCGGCAGTGTGTTTTTGCAACAACAACAAAGAGGGGTG GTGCACTTAAGAAAGGACCAAATGCCAAAGCATTGCAAGTCATGAAGCAGACATTACCCTATAGTGTGGC AGACCTTGAATGGGATGCAGGTCATAAAACCAATGTCCAGCAGTGTTACTGCTATTGTGGAGGCCCTGGA GACTGGTATTTGAAGATGCTACAGTGCTGCAAATGTAAGCAGTGGTTTCATGAGGCTTGTGTGCAATGCC TTCAAAAGCCAATGCTATTTGGAGACAGATTTTATACGTTTATATGCTCTGTCTGCAGTTCTGGACCAGA ATACCTCAAACGTCTACCATTACAGTGGGTAGATATAGCACACCTATGCCTTTACAACCTAAGTGTTATT CATAAGAAGAAATACTTTGATTCTGAACTTGAGCTTATGACATACATTAATGAAAACTGGGATAGATTGC ACCCTGGAGAGCTGGCAGACACACCAAAATCTGAAAGATATGAGCATGTTCTGGAGGCATTAAATGATTA CAAGACCATGTTTATGTCTGGGAAAGAAATAAAGAAGAAGAAGCATTTGTTTGGGTTGCGAATTCGTGTT CCTCCTGTGCCACCAAATGTGGCTTTCAAAGCAGAGAAAGAACCTGAAGGAACATCTCATGAATTTAAAA TTAAAGGCAGAAAGGCATCCAAACCTATATCTGATTCAAGGGAAGTAAGCAATGGCATAGAAAAAAAAGG AAAGAAAAAATCTGTAGGTCGTCCACCTGGCCCATATACAAGAAAAATGATTCAAAAAACTGCTGAGCCA CTTTTGGATAAGGAATCAATTTCAGAGAATCCTACTTTGGATTTACCTTGTTCTATAGGGAGAACTGAGG GAACTGCACATTCATCCAATACCTCAGATGTGGATTTCACGGGTGCTTCCAGTGCAAAAGAAACTACCTC GTCTAGCATTTCCAGGCATTATGGATTATCTGACTCCAGAAAAAGAACGCGTACAGGAAGATCTTGGCCT GCTGCAATACCACATTTGCGGAGAAGAAGAGGTCGTCTTCCAAGAAGAGCACTCCAGACTCAGAACTCAG AAATTGTAAAAGATGATGAAGGCAAAGAAGATTATCAGTTTGATGAACTCAACACAGAGATTCTGAATAA CTTAGCAGATCAGGAGTTACAACTCAATCATCTAAAGAACTCCATTACCAGTTATTTTGGTGCTGCAGGT AGAATAGCATGTGGCGAAAAATACCGAGTTTTGGCACGTCGGGTGACACTTGATGGAAAGGTGCAGTATC TTGTGGAATGGGAAGGAGCAACTGCATCCAGCGGACCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC221006 representing NM_007358
Red=Cloning site Green=Tags(s) MRDSTGAGNSLVHKRSPLRRNQKTPTSLTKLSLQDGHKAKKPACKFEEGQDVLARWSDGLFYLGTIKKIN ILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDS SVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPG DWYLKMLQCCKCKQWFHEACVQCLQKPMLFGDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVI HKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRV PPVPPNVAFKAEKEPEGTSHEFKIKGRKASKPISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEP LLDKESISENPTLDLPCSIGRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSWP AAIPHLRRRRGRLPRRALQTQNSEIVKDDEGKEDYQFDELNTEILNNLADQELQLNHLKNSITSYFGAAG RIACGEKYRVLARRVTLDGKVQYLVEWEGATASSGP SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007358 |
| ORF Size | 2174 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007358.4 |
| RefSeq Size | 2648 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 22823 |
| UniProt ID | Q9Y483 |
| Domains | PHD, TUDOR |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 66.9 kDa |
| Gene Summary | Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221006L3 | Lenti ORF clone of Human metal response element binding transcription factor 2 (MTF2), transcript variant 1, Myc-DDK-tagged |
CNY 7,130.00 |
|
| RC221006L4 | Lenti ORF clone of Human metal response element binding transcription factor 2 (MTF2), transcript variant 1, mGFP tagged |
CNY 7,130.00 |
|
| RG221006 | MTF2 (tGFP-tagged) - Human metal response element binding transcription factor 2 (MTF2), transcript variant 1 |
CNY 5,700.00 |
|
| SC115558 | MTF2 (untagged)-Human metal response element binding transcription factor 2 (MTF2), transcript variant 1 |
CNY 4,424.00 |
