IGSF3 (NM_001542) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EWI-3; LCDD; V8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC220966 representing NM_001542 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTGCTTTTTCCCGGTGCTGAGCTGTCTGGCTGTGCTGGGTGTGGTGTCAGCACAGCGGCAGGTCA CCGTTCAGGAAGGACCCTTGTACCGCACGGAGGGCTCCCACATCACTATCTGGTGCAATGTGAGTGGCTA CCAGGGACCTTCTGAGCAGAATTTCCAGTGGTCCATTTACCTGCCTTCGTCGCCAGAGCGAGAGGTGCAG ATCGTCAGCACCATGGACTCTTCCTTCCCCTATGCCATCTACACCCAGCGCGTCCGCGGAGGGAAGATCT TCATAGAAAGAGTCCAGGGGAACTCAACCCTATTGCACATCACAGATCTTCAGGCCCGGGATGCCGGGGA GTATGAATGCCACACACCCAGCACTGATAAGCAATACTTTGGGAGTTACAGTGCAAAGATGAACCTAGTG GTGATCCCAGACTCCCTGCAGACCACTGCCATGCCCCAGACTCTGCACAGAGTGGAGCAGGACCCGCTGG AGCTCACTTGTGAGGTGGCCTCAGAGACCATTCAGCACAGCCACCTGTCTGTGGCCTGGCTCCGGCAGAA AGTTGGCGAGAAGCCCGTGGAGGTCATCTCCCTGAGCCGAGATTTCATGCTTCACTCCAGCAGCGAATAT GCCCAGAGGCAGAGCCTGGGGGAGGTGCGGCTGGACAAGCTGGGGAGGACCACCTTCCGCCTCACCATCT TCCACCTGCAGCCTTCTGACCAGGGCGAATTCTACTGCGAGGCCGCCGAGTGGATCCAGGATCCGGATGG GTCGTGGTATGCTATGACCCGAAAGCGTTCCGAGGGAGCCGTGGTCAACGTCCAGCCAACTGACAAAGAA TTCACTGTTCGGCTGGAGACAGAGAAGCGGCTGCACACGGTGGGCGAGCCGGTGGAGTTCAGATGCATCC TGGAGGCTCAGAATGTTCCCGACCGTTACTTTGCTGTCTCCTGGGCCTTCAACAGCTCGCTCATCGCCAC CATGGGTCCTAACGCTGTGCCTGTCCTCAACAGCGAATTTGCTCACCGGGAAGCCAGGGGACAGCTTAAG GTGGCCAAAGAGAGCGACAGTGTCTTTGTGCTGAAGATCTACCACCTCCGCCAGGAAGATAGCGGGAAAT ACAACTGCCGGGTGACTGAGCGAGAGAAAACCGTGACCGGGGAATTCATTGATAAGGAGAGCAAGCGTCC CAAGAACATCCCCATCATAGTCCTCCCCCTCACAGATAACTGGGTAGTTAAAGTCCCCCAGCACCACCAG CTCCTGTCCCAAGGCCACTTGGAGAGCAGCATCTCCGTGGAGGTGGCCAGCAATGCCAGCGTCATCCTTG AGGGCGAGGACCTGCGCTTCTCCTGCAGTGTCCGCACGGCAGGCAGGCCGCAGGGTCGCTTCTCTGTCAT CTGGCAGCTTGTGGACAGGCAGAACCGCCGCAGCAATATCATGTGGCTAGACCGGGATGGCACCGTGCAG CCAGGCTCGTCCTACTGGGAGCGCAGCAGCTTTGGGGGCGTCCAGATGGAGCAGGTGCAGCCCAACTCGT TCAGCCTGGGCATCTTCAACAGCAGGAAGGAGGACGAGGGCCAGTATGAATGCCATGTGACTGAATGGGT GCGGGCAGTGGATGGCGAGTGGCAGATTGTTGGGGAGCGCCGGGCCAGCACTCCCATCTCCATCACAGCT CTTGAAATGGGCTTCGCAGTCACAGCCATCTCCCGGACACCGGGGGTGACCTACAGCGACTCCTTTGACT TGCAGTGTATCATCAAACCCCACTACCCTGCCTGGGTCCCCGTGTCGGTGACATGGCGGTTCCAGCCGGT GGGCACGGTGGAGTTCCATGACTTGGTGACCTTCACCCGGGACGGAGGGGTCCAGTGGGGGGACAGGTCC TCCAGCTTCCGAACCCGAACTGCCATCGAGAAGGCTGAGTCCAGCAACAACGTCCGCCTAAGCATCAGCC GAGCCAGTGACACGGAAGCAGGCAAGTACCAGTGTGTGGCAGAGCTGTGGCGGAAGAACTACAACAACAC CTGGACGCGACTGGCGGAGAGGACCTCCAACCTGCTGGAGATCAGGGTGCTGCAGCCAGTGACAAAGCTG CAGGTGAGCAAATCGAAGAGGACCCTCACCCTGGTGGAAAACAAGCCCATTCAGTTGAACTGCTCAGTCA AGTCTCAGACTAGCCAGAACTCCCACTTTGCGGTGCTCTGGTATGTCCACAAGCCCTCGGATGCCGATGG CAAGCTTATCCTGAAGACCACCCACAACTCCGCCTTTGAATACGGTACTTACGCCGAGGAGGAGGGCCTG AGAGCCAGGCTCCAGTTTGAGAGGCATGTGTCGGGGGGCCTGTTCAGCCTCACCGTCCAGAGAGCCGAGG TCAGCGACAGCGGCAGCTACTACTGCCACGTGGAGGAGTGGCTGCTGAGCCCCAACTACGCCTGGTACAA GCTGGCAGAGGAGGTTTCTGGGCGCACAGAAGTCACTGTGAAACAGCCAGACAGCCGCCTGAGGCTCAGC CAAGCCCAGGGGAACCTGTCGGTTCTGGAGACCCGGCAGGTACAGCTGGAGTGTGTGGTTCTCAACCGCA CCAGCATAACCTCCCAGCTCATGGTGGAATGGTTTGTATGGAAGCCCAACCACCCTGAGCGGGAGACTGT GGCCCGCTTGAGCCGTGACGCCACCTTCCACTATGGAGAGCAGGCAGCCAAGAACAATCTGAAGGGGCGG CTGCATTTGGAGAGTCCTTCCCCCGGCGTGTACCGTCTCTTCATCCAGAACGTGGCTGTGCAGGACAGCG GGACCTACAGCTGCCATGTGGAGGAGTGGCTGCCCAGCCCCAGTGGCATGTGGTATAAGCGGGCAGAGGA CACCGCTGGGCAGACAGCTCTGACAGTCATGCGACCAGATGCTTCCCTGCAGGTGGACACAGTGGTCCCC AATGCCACGGTCTCTGAGAAGGCAGCTTTCCAGCTGGACTGTAGCATCGTGTCCCGCTCCAGCCAGGACT CCCGCTTCGCTGTGGCCTGGTATTCCCTGAGGACTAAAGCTGGGGGGAAAAGGAGCAGCCCTGGCCTGGA AGAACAGGAAGAGGAAAGGGAGGAGGAGGAGGAGGAGGACGACGACGACGACGACGACCCAACAGAGCGG ACGGCCCTGCTGAGCGTGGGCCCAGATGCTGTCTTTGGCCCAGAGGGCAGTCCTTGGGAGGGCAGGCTTC GCTTCCAGAGGCTCTCCCCGGTGCTCTACCGGCTCACAGTGCTGCAGGCAAGCCCCCAAGATACAGGCAA TTACTCCTGCCATGTGGAGGAGTGGCTGCCCAGCCCTCAGAAGGAATGGTACCGGCTGACGGAGGAGGAG TCAGCCCCCATCGGCATCCGTGTTCTAGATACAAGTCCCACCCTCCAGTCCATCATCTGCTCCAACGACG CACTCTTCTACTTCGTCTTCTTCTACCCTTTCCCCATCTTTGGCATTCTTATCATCACCATCCTTCTGGT GCGTTTCAAGAGCCGGAACTCCAGCAAGAACTCTGATGGGAAGAATGGGGTGCCTCTGCTGTGGATCAAA GAGCCACACCTCAACTACTCCCCTACTTGCCTGGAGCCCCCTGTTCTCAGTATCCATCCAGGGGCCATAG AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC220966 representing NM_001542 Red=Cloning site Green=Tags(s) MKCFFPVLSCLAVLGVVSAQRQVTVQEGPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIYLPSSPEREVQ IVSTMDSSFPYAIYTQRVRGGKIFIERVQGNSTLLHITDLQARDAGEYECHTPSTDKQYFGSYSAKMNLV VIPDSLQTTAMPQTLHRVEQDPLELTCEVASETIQHSHLSVAWLRQKVGEKPVEVISLSRDFMLHSSSEY AQRQSLGEVRLDKLGRTTFRLTIFHLQPSDQGEFYCEAAEWIQDPDGSWYAMTRKRSEGAVVNVQPTDKE FTVRLETEKRLHTVGEPVEFRCILEAQNVPDRYFAVSWAFNSSLIATMGPNAVPVLNSEFAHREARGQLK VAKESDSVFVLKIYHLRQEDSGKYNCRVTEREKTVTGEFIDKESKRPKNIPIIVLPLTDNWVVKVPQHHQ LLSQGHLESSISVEVASNASVILEGEDLRFSCSVRTAGRPQGRFSVIWQLVDRQNRRSNIMWLDRDGTVQ PGSSYWERSSFGGVQMEQVQPNSFSLGIFNSRKEDEGQYECHVTEWVRAVDGEWQIVGERRASTPISITA LEMGFAVTAISRTPGVTYSDSFDLQCIIKPHYPAWVPVSVTWRFQPVGTVEFHDLVTFTRDGGVQWGDRS SSFRTRTAIEKAESSNNVRLSISRASDTEAGKYQCVAELWRKNYNNTWTRLAERTSNLLEIRVLQPVTKL QVSKSKRTLTLVENKPIQLNCSVKSQTSQNSHFAVLWYVHKPSDADGKLILKTTHNSAFEYGTYAEEEGL RARLQFERHVSGGLFSLTVQRAEVSDSGSYYCHVEEWLLSPNYAWYKLAEEVSGRTEVTVKQPDSRLRLS QAQGNLSVLETRQVQLECVVLNRTSITSQLMVEWFVWKPNHPERETVARLSRDATFHYGEQAAKNNLKGR LHLESPSPGVYRLFIQNVAVQDSGTYSCHVEEWLPSPSGMWYKRAEDTAGQTALTVMRPDASLQVDTVVP NATVSEKAAFQLDCSIVSRSSQDSRFAVAWYSLRTKAGGKRSSPGLEEQEEEREEEEEEDDDDDDDPTER TALLSVGPDAVFGPEGSPWEGRLRFQRLSPVLYRLTVLQASPQDTGNYSCHVEEWLPSPQKEWYRLTEEE SAPIGIRVLDTSPTLQSIICSNDALFYFVFFYPFPIFGILIITILLVRFKSRNSSKNSDGKNGVPLLWIK EPHLNYSPTCLEPPVLSIHPGAID TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001542
ORF Size	3642 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001542.4
RefSeq Size	7253 bp
RefSeq ORF	3645 bp
Locus ID	3321
UniProt ID	O75054
Domains	ig, IGv, IG
Protein Families	Transmembrane
MW	137.3 kDa
Gene Summary	The protein encoded by this gene is an immunoglobulin-like membrane protein containing several V-type Ig-like domains. A mutation in this gene has been associated with bilateral nasolacrimal duct obstruction (LCDD). [provided by RefSeq, Jun 2016]

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