PHF3 (NM_015153) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC220863 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATAGTTGATACATTTAATCATTTAATTCCTACTGAACACTTAGATGATGCCCTATTTCTAGGAT CCAACCTGGAGAATGAAGTCTGTGAGGATTTTAGTGCAAGTCAAAATGTCTTAGAGGACTCGCTGAAGAA CATGCTCAGCGATAAGGATCCTATGCTAGGATCTGCAAGTAACCAGTTCTGTTTGCCTGTTTTGGATAGC AATGATCCCAATTTCCAGATGCCTTGTTCAACAGTTGTTGGTCTTGACGATATTATGGATGAAGGAGTTG TTAAAGAAAGTGGCAATGATACCATTGATGAAGAAGAACTGATTTTACCTAACAGGAACTTAAGGGACAA GGTAGAAGAAAATTCAGTGAGATCTCCAAGAAAATCACCTCGTTTAATGGCACAAGAACAAGTAAGAAGT TTGCGACAGAGCACTATTGCCAAGCGTTCAAATGCAGCACCATTAAGTAACACAAAAAAAGCATCTGGGA AGACTGTATCTACTGCTAAAGCAGGAGTGAAACAACCAGAAAGGAGTCAGGTTAAAGAAGAAGTATGTAT GTCACTGAAACCTGAGTACCATAAGGAGAATAGAAGGTGCAGCCGAAATAGCGGACAAATTGAAGTGGTA CCTGAAGTATCAGTGTCTTCAAGTCATTCTTCAGTGTCATCTTGTCTTGAAATGAAGGATGAAGATGGAT TAGATTCTAAGCATAAGTGTAATAATCCGGGAGAAATAGATGTGCCATCTCATGAATTAAATTGTTCACT TCTTTCAGAGACTTGTGTTACTATTGGAGAAAAGAAAAATGAAGCTTTGATGGAATGTAAAGCCAAGCCT GTTGGTAGTCCATTGTTTAAGTTTTCAGATAAAGAAGAACATGAACAAAATGATTCCATTTCAGGTAAAA CGGGTGAGACTGTTGTTGAAGAAATGATAGCAACAAGAAAAGTTGAACAAGATTCAAAGGAGACAGTAAA ATTATCCCATGAAGATGACCATATTCTTGAGGACGCTGGATCTTCTGATATTTCTAGTGATGCTGCTTGT ACAAATCCAAATAAGACAGAAAACAGCCTTGTAGGTTTGCCTAGTTGTGTAGATGAAGTGACTGAATGTA ATTTGGAATTGAAGGATACCATGGGTATTGCTGATAAAACTGAGAACACCCTTGAAAGAAATAAAATTGA ACCGTTGGGTTATTGTGAAGATGCGGAGTCTAATAGGCAGTTGGAGAGCACTGAGTTTAATAAATCAAAC TTAGAGGTGGTTGATACTAGTACTTTTGGACCGGAAAGTAATATCTTGGAAAATGCTATTTGTGATGTGC CTGACCAAAATTCAAAACAGTTGAATGCTATAGAAAGTACTAAAATAGAGTCCCATGAAACAGCAAACCT TCAGGATGACAGAAACAGCCAGTCAAGTAGCGTTTCTTACTTAGAGTCAAAAAGTGTAAAATCCAAACAT ACAAAACCTGTAATTCATTCTAAGCAAAACATGACCACAGATGCTCCGAAGAAAATTGTTGCAGCAAAGT ATGAAGTAATACATAGCAAAACTAAAGTTAATGTCAAAAGTGTGAAACGAAATACTGATGTACCAGAATC TCAGCAAAATTTTCATAGGCCAGTCAAAGTCAGAAAAAAACAAATTGATAAGGAGCCAAAGATTCAGAGT TGCAATTCTGGGGTTAAATCTGTGAAAAACCAAGCTCATTCTGTACTGAAAAAAACATTACAGGATCAAA CTTTAGTACAAATTTTCAAGCCCTTAACTCATTCTTTGAGTGATAAGTCACACGCTCATCCTGGTTGCTT GAAAGAACCTCATCATCCTGCACAAACTGGACATGTATCACATTCTAGCCAGAAACAGTGTCATAAGCCT CAGCAACAGGCCCCAGCAATGAAAACCAATAGTCACGTGAAGGAAGAGCTTGAACACCCAGGCGTTGAGC ATTTTAAGGAAGAGGATAAACTGAAACTGAAAAAACCTGAGAAGAACCTACAACCCCGCCAAAGAAGAAG CAGCAAAAGTTTTTCTTTAGATGAGCCACCATTGTTCATTCCAGATAACATAGCTACCATAAGAAGAGAA GGCTCTGATCATAGCTCCTCATTTGAAAGCAAATATATGTGGACTCCCAGCAAGCAGTGTGGGTTTTGCA AAAAACCACATGGCAACAGGTTTATGGTTGGCTGTGGGAGATGTGATGACTGGTTTCATGGTGATTGTGT TGGGTTAAGTCTTTCTCAAGCACAGCAGATGGGCGAGGAAGACAAAGAATATGTCTGTGTAAAATGTTGT GCTGAAGAAGACAAAAAGACTGAAATACTAGATCCAGATACTTTGGAAAACCAAGCTACAGTTGAATTCC ATAGTGGAGATAAAACAATGGAGTGTGAAAAGCTTGGATTATCAAAACACACAACAAATGATAGAACCAA ATATATAGATGATACAGTGAAGCACAAGGTCAAAATTTTAAAACGGGAGTCTGGTGAAGGCAGAAATTCA TCAGACTGTAGAGATAATGAAATTAAAAAATGGCAGCTAGCTCCTCTTCGTAAGATGGGACAACCAGTTT TACCTCGGAGATCCTCAGAAGAAAAAAGTGAAAAAATACCGAAAGAGTCTACAACTGTTACTTGCACAGG AGAAAAAGCTTCAAAACCAGGTACTCATGAGAAGCAAGAGATGAAAAAGAAGAAAGTTGAAAAAGGAGTG CTTAATGTACATCCTGCTGCTTCTGCTTCCAAGCCTTCTGCAGATCAGATCAGGCAAAGTGTCAGACATT CTCTCAAAGACATTCTTATGAAGAGACTTACAGACTCAAATTTGAAGGTACCAGAGGAAAAGGCAGCAAA AGTTGCCACAAAAATTGAGAAAGAGCTTTTCTCTTTTTTTCGGGACACAGATGCTAAATATAAGAACAAA TATAGAAGTTTGATGTTTAATTTGAAAGATCCTAAAAACAATATATTATTTAAAAAAGTACTGAAAGGAG AAGTAACTCCTGATCATCTTATCAGAATGAGTCCAGAAGAACTAGCTTCTAAAGAGTTAGCTGCTTGGAG ACGAAGAGAAAACAGACATACCATAGAAATGATTGAGAAAGAGCAGAGAGAAGTGGAACGACGGCCAATC ACCAAAATAACTCATAAAGGTGAAATAGAAATTGAGAGTGATGCCCCAATGAAAGAACAGGAAGCAGCCA TGGAGATTCAGGAACCAGCCGCCAATAAGTCATTGGAGAAGCCAGAAGGATCTGAAAAACAAAAAGAGGA GGTTGACTCTATGTCTAAAGATACCACTAGTCAACACAGACAGCATCTTTTTGATCTCAACTGCAAAATC TGCATAGGTCGAATGGCACCACCTGTAGATGATCTTTCTCCAAAAAAAGTAAAAGTTGTTGTAGGAGTAG CTCGCAAACATTCAGACAATGAAGCAGAAAGTATAGCAGATGCATTATCTTCAACCTCAAATATTTTGGC TTCTGAATTCTTTGAGGAGGAGAAACAGGAGTCTCCAAAGTCAACGTTCTCTCCTGCTCCACGTCCAGAG ATGCCTGGAACTGTTGAAGTTGAGTCTACCTTTCTGGCTCGATTGAACTTCATCTGGAAAGGTTTTATCA ACATGCCTTCTGTGGCAAAATTTGTTACCAAAGCCTATCCAGTATCTGGCTCCCCAGAATACCTGACAGA GGACCTACCAGATAGTATTCAAGTAGGTGGCAGGATATCACCTCAGACAGTTTGGGATTATGTGGAAAAA ATAAAAGCATCAGGAACCAAGGAAATTTGTGTGGTTCGCTTCACACCAGTAACTGAAGAAGATCAAATTT CTTATACTTTGCTCTTTGCATACTTCAGTAGCAGAAAGCGCTATGGAGTAGCTGCTAACAACATGAAGCA GGTTAAAGATATGTACCTTATTCCTTTGGGTGCCACAGATAAAATTCCACACCCTCTTGTGCCTTTTGAT GGACCTGGGCTTGAACTGCATAGACCTAATCTATTGTTGGGCTTAATTATTCGTCAGAAACTGAAGCGAC AGCACAGTGCCTGTGCTAGTACTAGTCATATAGCTGAGACTCCTGAAAGTGCACCACCAATAGCATTGCC ACCTGATAAAAAAAGTAAAATAGAAGTTTCTACAGAAGAAGCACCAGAGGAAGAAAATGACTTTTTTAAT TCTTTTACAACTGTATTACACAAGCAGAGAAATAAACCTCAGCAGAATCTTCAGGAAGACCTTCCAACAG CAGTTGAACCTTTAATGGAAGTCACCAAACAGGAGCCACCAAAACCTTTAAGATTTCTTCCTGGCGTGTT GATTGGCTGGGAGAATCAACCTACTACTCTGGAATTAGCAAATAAACCTCTTCCTGTGGATGATATACTT CAAAGCCTTTTGGGCACCACTGGTCAAGTATATGACCAGGCCCAGTCAGTGATGGAACAAAACACTGTTA AAGAAATTCCATTTTTAAATGAGCAGACCAACTCAAAAATAGAGAAAACAGATAATGTGGAAGTAACTGA TGGTGAAAACAAGGAGATAAAAGTTAAAGTAGATAATATTTCAGAATCTACAGATAAGTCAGCAGAAATA GAAACATCAGTAGTAGGGTCCTCTTCCATTTCTGCAGGGTCTTTGACGAGTCTTAGTCTCAGAGGTAAGC CACCAGATGTTTCTACAGAAGCATTTTTAACAAATTTATCAATTCAGTCAAAACAAGAGGAAACTGTGGA GAGTAAAGAGAAAACATTAAAAAGACAGCTTCAGGAAGATCAAGAGAATAATTTGCAAGATAACCAGACT TCAAATAGTTCTCCATGCAGATCTAATGTAGGAAAAGGAAACATAGATGGTAATGTGAGCTGTAGTGAAA ACCTTGTTGCTAATACAGCGAGGTCTCCACAGTTTATCAACCTGAAAAGGGATCCTAGGCAAGCAGCAGG ACGAAGTCAGCCTGTAACTACTTCAGAAAGCAAAGATGGAGATAGTTGCCGGAATGGAGAAAAACACATG CTGCCTGGCCTGTCACACAACAAGGAGCACTTAACAGAACAAATCAATGTAGAGGAAAAGTTGTGTTCTG CAGAGAAAAACTCGTGTGTTCAGCAGAGTGACAATTTAAAAGTTGCACAAAACTCACCATCAGTAGAAAA CATACAGACTTCTCAAGCAGAACAAGCAAAACCCTTACAGGAGGATATTTTAATGCAAAATATTGAAACT GTGCACCCATTTCGAAGAGGATCAGCAGTAGCGACATCTCATTTTGAAGTTGGAAACACATGTCCATCAG AATTTCCTTCTAAAAGCATCACCTTTACTTCCAGAAGCACCAGCCCCAGAACAAGTACAAACTTTTCACC CATGAGGCCACAGCAGCCCAACCTTCAGCATCTCAAGTCTAGCCCACCTGGATTTCCATTTCCAGGGCCT CCTAATTTTCCCCCACAAAGCATGTTTGGATTTCCACCACATTTGCCACCTCCATTACTTCCCCCTCCAG GCTTTGGCTTTGCTCAAAATCCCATGGTTCCCTGGCCACCTGTTGTTCATCTCCCAGGTCAGCCACAGCG TATGATGGGTCCTCTCTCACAAGCATCAAGGTATATAGGCCCGCAGAATTTTTACCAGGTTAAAGACATT CGGAGGCCAGAAAGGCGCCATAGTGACCCTTGGGGTAGGCAAGACCAACAGCAACTGGATAGGCCATTTA ATAGGGGTAAAGGGGACCGCCAGAGATTTTATAGTGATTCACACCATTTGAAAAGAGAGCGACATGAAAA GGAATGGGAGCAAGAATCTGAAAGGCATAGACGCAGAGACAGAAGCCAAGACAAGGACAGAGACAGAAAA AGCAGGGAGGAAGGGCACAAAGATAAAGAGAGGGCACGGTTATCACATGGTGATCGAGGAACAGATGGAA AAGCAAGCAGAGATAGTAGGAATGTAGACAAGAAGCCAGATAAACCTAAAAGTGAAGACTATGAGAAGGA CAAAGAACGAGAGAAAAGTAAACACAGAGAAGGAGAAAAGGACAGGGATAGGTACCACAAAGATAGGGAC CACACTGACAGAACTAAAAGCAAAAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC220863 protein sequence Red=Cloning site Green=Tags(s) MDIVDTFNHLIPTEHLDDALFLGSNLENEVCEDFSASQNVLEDSLKNMLSDKDPMLGSASNQFCLPVLDS NDPNFQMPCSTVVGLDDIMDEGVVKESGNDTIDEEELILPNRNLRDKVEENSVRSPRKSPRLMAQEQVRS LRQSTIAKRSNAAPLSNTKKASGKTVSTAKAGVKQPERSQVKEEVCMSLKPEYHKENRRCSRNSGQIEVV PEVSVSSSHSSVSSCLEMKDEDGLDSKHKCNNPGEIDVPSHELNCSLLSETCVTIGEKKNEALMECKAKP VGSPLFKFSDKEEHEQNDSISGKTGETVVEEMIATRKVEQDSKETVKLSHEDDHILEDAGSSDISSDAAC TNPNKTENSLVGLPSCVDEVTECNLELKDTMGIADKTENTLERNKIEPLGYCEDAESNRQLESTEFNKSN LEVVDTSTFGPESNILENAICDVPDQNSKQLNAIESTKIESHETANLQDDRNSQSSSVSYLESKSVKSKH TKPVIHSKQNMTTDAPKKIVAAKYEVIHSKTKVNVKSVKRNTDVPESQQNFHRPVKVRKKQIDKEPKIQS CNSGVKSVKNQAHSVLKKTLQDQTLVQIFKPLTHSLSDKSHAHPGCLKEPHHPAQTGHVSHSSQKQCHKP QQQAPAMKTNSHVKEELEHPGVEHFKEEDKLKLKKPEKNLQPRQRRSSKSFSLDEPPLFIPDNIATIRRE GSDHSSSFESKYMWTPSKQCGFCKKPHGNRFMVGCGRCDDWFHGDCVGLSLSQAQQMGEEDKEYVCVKCC AEEDKKTEILDPDTLENQATVEFHSGDKTMECEKLGLSKHTTNDRTKYIDDTVKHKVKILKRESGEGRNS SDCRDNEIKKWQLAPLRKMGQPVLPRRSSEEKSEKIPKESTTVTCTGEKASKPGTHEKQEMKKKKVEKGV LNVHPAASASKPSADQIRQSVRHSLKDILMKRLTDSNLKVPEEKAAKVATKIEKELFSFFRDTDAKYKNK YRSLMFNLKDPKNNILFKKVLKGEVTPDHLIRMSPEELASKELAAWRRRENRHTIEMIEKEQREVERRPI TKITHKGEIEIESDAPMKEQEAAMEIQEPAANKSLEKPEGSEKQKEEVDSMSKDTTSQHRQHLFDLNCKI CIGRMAPPVDDLSPKKVKVVVGVARKHSDNEAESIADALSSTSNILASEFFEEEKQESPKSTFSPAPRPE MPGTVEVESTFLARLNFIWKGFINMPSVAKFVTKAYPVSGSPEYLTEDLPDSIQVGGRISPQTVWDYVEK IKASGTKEICVVRFTPVTEEDQISYTLLFAYFSSRKRYGVAANNMKQVKDMYLIPLGATDKIPHPLVPFD GPGLELHRPNLLLGLIIRQKLKRQHSACASTSHIAETPESAPPIALPPDKKSKIEVSTEEAPEEENDFFN SFTTVLHKQRNKPQQNLQEDLPTAVEPLMEVTKQEPPKPLRFLPGVLIGWENQPTTLELANKPLPVDDIL QSLLGTTGQVYDQAQSVMEQNTVKEIPFLNEQTNSKIEKTDNVEVTDGENKEIKVKVDNISESTDKSAEI ETSVVGSSSISAGSLTSLSLRGKPPDVSTEAFLTNLSIQSKQEETVESKEKTLKRQLQEDQENNLQDNQT SNSSPCRSNVGKGNIDGNVSCSENLVANTARSPQFINLKRDPRQAAGRSQPVTTSESKDGDSCRNGEKHM LPGLSHNKEHLTEQINVEEKLCSAEKNSCVQQSDNLKVAQNSPSVENIQTSQAEQAKPLQEDILMQNIET VHPFRRGSAVATSHFEVGNTCPSEFPSKSITFTSRSTSPRTSTNFSPMRPQQPNLQHLKSSPPGFPFPGP PNFPPQSMFGFPPHLPPPLLPPPGFGFAQNPMVPWPPVVHLPGQPQRMMGPLSQASRYIGPQNFYQVKDI RRPERRHSDPWGRQDQQQLDRPFNRGKGDRQRFYSDSHHLKRERHEKEWEQESERHRRRDRSQDKDRDRK SREEGHKDKERARLSHGDRGTDGKASRDSRNVDKKPDKPKSEDYEKDKEREKSKHREGEKDRDRYHKDRD HTDRTKSKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015153
ORF Size	6117 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015153.4
RefSeq Size	7960 bp
RefSeq ORF	6120 bp
Locus ID	23469
UniProt ID	Q92576
Domains	PHD, TFS2M
Protein Families	Transcription Factors
MW	229.5 kDa
Gene Summary	This gene encodes a member of a PHD finger-containing gene family. This gene may function as a transcription factor and may be involved in glioblastomas development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

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