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MAP4K1 (NM_007181) Human Tagged ORF Clone
CAT＃: RC220698
MAP4K1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2
ORF Plasmid: tGFP
"NM_007181" in other vectors (7)
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CNY 6,104.00
CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HPK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220698 representing NM_007181
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGG GTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGAT GGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGG CACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCT GTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGT CTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGA GCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGG CTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGC CCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAG CTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGC CTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAA GAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAAT CGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTG AGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCT GGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGA CCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGT CAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACT TCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTG AGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCAT CCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGA CAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTG TTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACC TGCTCCTGGGGGCAGAGGAAGGCATCTTCATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCT CTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCC CACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTC ACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTG CCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCC GTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGC CGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGT GAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTG GGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGT TGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGAT CCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGA GTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTC TGCTTGGCTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCGCCTCACTGCAACCTCCTCCTCCCAGGTTC AAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220698 representing NM_007181
Red=Cloning site Green=Tags(s) MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCR HANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKG ANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAE LQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLN RGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETR PPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQL SPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKL FNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTP HLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETS VVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWL GEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHG VQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007181 |
| ORF Size | 2499 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007181.6 |
| RefSeq Size | 2732 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 11184 |
| UniProt ID | Q92918 |
| Domains | pkinase, CNH, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| MW | 91.1 kDa |
| Gene Summary | Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220698L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged |
CNY 8,504.00 |
|
| RC220698L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged |
CNY 10,640.00 |
|
| RC220698L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, Myc-DDK-tagged |
CNY 8,504.00 |
|
| RC220698L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mGFP tagged |
CNY 8,504.00 |
|
| RG220698 | MAP4K1 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 |
CNY 9,600.00 |
|
| SC310086 | MAP4K1 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 |
CNY 16,060.00 |
|
| SC323528 | MAP4K1 (untagged)-Kinase deficient mutant (K46M) of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2 |
CNY 6,112.00 |
