ZNF274 (NM_016325) Human Tagged ORF Clone
CAT#: RC220631
- TrueORF®
ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a
ORF Plasmid: tGFP
"NM_016325" in other vectors (4)
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CNY 4,624.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HFB101; ZF2; ZKSCAN19; ZSCAN51 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220631 representing NM_016325
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCAGGCTTCCGACGGCCTGGTCCTGTGAACCAGTGACCTTTGAAGATGTAACACTGGGTTTTA CCCCGGAAGAGTGGGGACTGCTGGACCTCAAACAGAAGTCCCTGTACAGGGAAGTGATGCTGGAGAACTA CAGGAACCTGGTCTCAGTGGAGTATCCTGAGCTCCAGCTGGACCCTAAATTGGATCCTCTTCCTGCTGAG AGTCCCCTAATGAACATTGAGGTTGTTGAGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATG CCCAGATCCAGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTCCAACA GCAGGGCAAGCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACATG ACAGGTCCCCGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGCCTAAGGCACGCT CCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTAGGTGCACTGCCTGTGAAGCTCCGGAC ATGGGTGGAATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGGCCCTGGTAGAGGGTGTGACCTGGATG TCTGAGGAGGAAGTACTTCCTGCAGGACAACCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCC AGCAGGAGGAGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTT CCAGGATGTGGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGTAC CGCGATGTGATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACACTGGAAAATAAAG AGTTAGCTCCAAATTCTGACATTCCTGAGGAAGAACCAGCCCCCAGCCTGAAAGTACAAGAATCCTCAAG GGATTGTGCCTTGTCCTCTACATTAGAAGATACCTTGCAGGGTGGGGTCCAGGAAGTCCAAGACACAGTG TTGAAGCAGATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCC AGGCAAACAGTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGC AAACAGTGGCGCTCTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCAAA CGTGACTCACAAGTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGC AAAAGGCCAAGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTACTAAACAGATTACGTTTATAAG AATTCACAAGGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTAAAATATTCCGGAACCCAAGATACTTT TCTGTGCATAAGAAAATCCATACCGGAGAGAGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTC AGAGCTCTTCCCTCACACAGCATCAGAGAGTTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGG GAGGACCTTCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACAAG TGTCAGGACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATCAGACATCAGAGGACTCACACCGGGG AGCGCCCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAGAGCTCACACCTTATTGGGCACCAGAG AACCCACAATAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220631 representing NM_016325
Red=Cloning site Green=Tags(s) MASRLPTAWSCEPVTFEDVTLGFTPEEWGLLDLKQKSLYREVMLENYRNLVSVEYPELQLDPKLDPLPAE SPLMNIEVVEVLTLNQEVAGPRNAQIQALYAEDGSLSADAPSEQVQQQGKHPGDPEAARQRFRQFRYKDM TGPREALDQLRELCHQWLQPKARSKEQILELLVLEQFLGALPVKLRTWVESQHPENCQEVVALVEGVTWM SEEEVLPAGQPAEGTTCCLEVTAQQEEKQEDAAICPVTVLPEEPVTFQDVAVDFSREEWGLLGPTQRTEY RDVMLETFGHLVSVGWETTLENKELAPNSDIPEEEPAPSLKVQESSRDCALSSTLEDTLQGGVQEVQDTV LKQMESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQKIDNPESQANSGALDTNQVLLHKIPPRKRLRK RDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYF SVHKKIHTGERPYVCQDCGKGFVQSSSLTQHQRVHSGERPFECQECGRTFNDRSAISQHLRTHTGAKPYK CQDCGKAFRQSSHLIRHQRTHTGERPYACNKCGKAFTQSSHLIGHQRTHNRTKRKKKQPTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016325 |
ORF Size | 1863 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016325.3, NP_057409.1 |
RefSeq Size | 2743 bp |
RefSeq ORF | 1866 bp |
Locus ID | 10782 |
UniProt ID | Q96GC6 |
Protein Families | Transcription Factors |
Protein Pathways | Neurotrophin signaling pathway |
MW | 70.3 kDa |
Gene Summary | This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220631L3 | Lenti-ORF clone of ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 6,460.00 |
|
RC220631L4 | Lenti-ORF clone of ZNF274 (mGFP-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 6,460.00 |
|
RG220631 | ZNF274 (tGFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 5,040.00 |
|
SC314227 | ZNF274 (untagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a |
CNY 9,980.00 |