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ARHGAP22 (NM_021226) Human Tagged ORF Clone
CAT＃: RC220614
ARHGAP22 (Myc-DDK-tagged)-Human Rho GTPase activating protein 22 (ARHGAP22)
ORF Plasmid: tGFP
"NM_021226" in other vectors (6)
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CNY 7,672.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RhoGAP2; RhoGap22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220614 representing NM_021226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGCCCAAAGATCAGGCAGGCCAGGAGGGCCCGCTCCAAAAGCCTAGTGATGGGGGAGCAGAGCC GGAGCCCTGGGCGGATGCCGTGCCCTCACAGGCTGGGCCCCGTGCTGAAGGCGGGCTGGCTGAAGAAGCA GAGGAGCATCATGAAGAACTGGCAGCAGCGCTGGTTTGTGCTGCGTGGGGATCAGCTTTTCTACTACAAG GACAAAGATGAGATCAAGCCCCAGGGATTTATTTCTCTACAAGGGACACAGGTGACTGAACTTCCTCCTG GCCCCGAGGACCCAGGGAAGCACCTCTTTGAGATCAGCCCAGGTGGTGCCGGGGAGCGGGAGAAGGTGCC GGCCAACCCCGAGGCGCTCCTGCTCATGGCCAGCTCCCAGCGTGACATGGAGGACTGGGTGCAGGCCATC CGCCGAGTCATCTGGGCCCCGCTGGGCGGAGGGATCTTTGGGCAGCGCCTAGAGGAAACAGTCCACCACG AGCGGAAGTATGGCCCCCGCCTGGCGCCCCTGCTGGTGGAGCAGTGTGTGGACTTCATCCGGGAGCGCGG GCTCACTGAGGAGGGGCTGTTCCGCATGCCAGGCCAGGCCAACCTGGTGAGGGACCTGCAGGATTCCTTC GACTGTGGGGAGAAGCCACTGTTTGACAGCACAACAGACGTGCACACGGTGGCCTCCCTGCTGAAGCTGT ACCTGCGGGAGCTCCCCGAGCCCGTGGTCCCCTTCGCCAGGTACGAGGACTTCCTCAGCTGCGCCCAGCT GCTCACCAAGGACGAGGGGGAGGGCACTCTGGAGTTGGCTAAACAAGTGAGCAACCTTCCTCAGGCAAAT TACAACCTGCTCAGATACATCTGCAAGTTTCTGGATGAAGTTCAGGCATACTCAAATGTCAACAAGATGA GTGTCCAGAATCTGGCAACCGTTTTTGGACCTAACATTCTGCGGCCACAGGTAGAGGACCCAGTAACCAT CATGGAAGGCACTTCCCTCGTCCAGCACCTGATGACCGTCCTCATCCGCAAACACAGCCAGCTCTTCACG GCACCGGTCCCGGAAGGGCCCACCTCCCCGCGCGGGGGCCTGCAATGCGCAGTGGGGTGGGGCTCCGAGG AGGTCACCAGGGACAGCCAAGGAGAGCCCGGCGGCCCCGGCCTGCCCGCGCACAGGACCTCTTCCCTGGA CGGGGCGGCCGTGGCGGTGCTCTCCAGAACAGCCCCCACGGGGCCGGGGAGCCGGTGCAGCCCTGGGAAG AAGGTGCAGACCCTGCCCAGTTGGAAGTCCTCCTTCCGGCAGCCGAGGTCCCTATCGGGAAGCCCGAAGG GGGGCGGCTCATCCCTGGAGGTGCCCATCATCTCCTCCGGCGGGAACTGGCTTATGAACGGGCTGTCCTC CCTGCGCGGACACCGCCGGGCCTCGTCGGGAGACCGGCTCAAGGACTCGGGCTCCGTGCAGAGACTCTCC ACCTACGACAATGTGCCCGCGCCGGGCCTGGTCCCCGGCATACCCAGCGTGGCCAGTATGGCGTGGTCCG GGGCCTCGTCCAGCGAGTCGTCGGTGGGGGGCTCACTCAGCAGCTGCACGGCCTGCCGCGCCAGCGACTC GTCTGCCCGCAGTTCCCTGCACACCGACTGGGCCCTGGAGCCCTCCCCGCTCCCCAGCAGCAGCGAGGAC CCCAAGTCCCTGGACCTGGACCACAGCATGGACGAGGCGGGCGCGGGTGCCAGCAACAGCGAGCCCAGCG AGCCGGACAGCCCCACCCGGGAACACGCGCGCCGCTCCGAGGCCTTACAGGGGCTGGTCACTGAGCTCAG GGCCGAGCTGTGCCGCCAGCGGACTGAGTACGAGAGGAGTGTGAAAAGAATCGAAGAAGGGAGTGCTGAC CTGAGAAAACGAATGTCCCGGTTAGAAGAAGAACTGGACCAGGAAAAGAAAAAATACATCATGCTGGAAA TAAAGCTGCGGAACTCTGAACGGGCGCGGGAGGATGCGGAGAGGAGGAACCAGCTGTTGCAGAGGGAAAT GGAGGAGTTTTTTTCGACCCTAGGAAGCTTGACTGTTGGGGCAAAAGGTGCCAGGGCCCCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220614 representing NM_021226
Red=Cloning site Green=Tags(s) MLSPKIRQARRARSKSLVMGEQSRSPGRMPCPHRLGPVLKAGWLKKQRSIMKNWQQRWFVLRGDQLFYYK DKDEIKPQGFISLQGTQVTELPPGPEDPGKHLFEISPGGAGEREKVPANPEALLLMASSQRDMEDWVQAI RRVIWAPLGGGIFGQRLEETVHHERKYGPRLAPLLVEQCVDFIRERGLTEEGLFRMPGQANLVRDLQDSF DCGEKPLFDSTTDVHTVASLLKLYLRELPEPVVPFARYEDFLSCAQLLTKDEGEGTLELAKQVSNLPQAN YNLLRYICKFLDEVQAYSNVNKMSVQNLATVFGPNILRPQVEDPVTIMEGTSLVQHLMTVLIRKHSQLFT APVPEGPTSPRGGLQCAVGWGSEEVTRDSQGEPGGPGLPAHRTSSLDGAAVAVLSRTAPTGPGSRCSPGK KVQTLPSWKSSFRQPRSLSGSPKGGGSSLEVPIISSGGNWLMNGLSSLRGHRRASSGDRLKDSGSVQRLS TYDNVPAPGLVPGIPSVASMAWSGASSSESSVGGSLSSCTACRASDSSARSSLHTDWALEPSPLPSSSED PKSLDLDHSMDEAGAGASNSEPSEPDSPTREHARRSEALQGLVTELRAELCRQRTEYERSVKRIEEGSAD LRKRMSRLEEELDQEKKKYIMLEIKLRNSERAREDAERRNQLLQREMEEFFSTLGSLTVGAKGARAPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021226 |
| ORF Size | 2094 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021226.4 |
| RefSeq Size | 2775 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 58504 |
| UniProt ID | Q7Z5H3 |
| MW | 76.6 kDa |
| Gene Summary | This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220614L1 | Lenti ORF clone of Human Rho GTPase activating protein 22 (ARHGAP22), Myc-DDK-tagged |
CNY 10,072.00 |
|
| RC220614L2 | Lenti ORF clone of Human Rho GTPase activating protein 22 (ARHGAP22), mGFP tagged |
CNY 7,700.00 |
|
| RC220614L3 | Lenti ORF clone of Human Rho GTPase activating protein 22 (ARHGAP22), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC220614L4 | Lenti ORF clone of Human Rho GTPase activating protein 22 (ARHGAP22), mGFP tagged |
CNY 7,700.00 |
|
| RG220614 | ARHGAP22 (tGFP-tagged) - Human Rho GTPase activating protein 22 (ARHGAP22) |
CNY 6,370.00 |
|
| SC304925 | ARHGAP22 (untagged)-Human Rho GTPase activating protein 22 (ARHGAP22) |
CNY 7,680.00 |
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