Protein kinase like protein SgK493 (PKDCC) (NM_138370) Human Tagged ORF Clone

CAT#: RC220604

PKDCC (Myc-DDK-tagged)-Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC)

ORF Plasmid: DDK tGFP



  "NM_138370" in other vectors (7)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal VLK Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RLSDF; SGK493; Vlk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220604 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTGCTGGAGCGGCTGCGGCACCCCAACGTGCTGCAGCTCTATGGCTACTGCTACCAGGACAGCG
AGGACATCCCAGACACCCTGACCACCATCACGGAGCTGGGCGCCCCTGTAGAAATGATCCAGCTGCTGCA
AACTTCCTGGGAGGATCGATTCCGAATCTGCCTGAGCCTGGGCCGCCTCCTCCACCACCTGGCCCACTCC
CCACTGGGCTCCGTCACTCTGCTGGACTTCCGCCCTCGGCAGTTTGTGCTGGTGGATGGGGAGCTCAAAG
TGACGGACCTGGATGACGCACGTGTGGAGGAGACGCCGTGTGCAGGCAGCACCGACTGCATACTCGAGTT
TCCGGCCAGGAACTTCACCCTGCCCTGCTCAGCCCAGGGCTGGTGCGAGGGCATGAACGAGAAGCGGAAC
CTCTATAATGCCTACAGGTTTTTCTTCACATACCTCCTGCCTCACAGTGCCCCGCCTTCACTGCGTCCTC
TGCTGGACAGCATCGTCAACGCCACAGGAGAGCTCGCCTGGGGGGTGGACGAGACCCTGGCCCAGCTGGA
GAAGGTGCTGCACCTGTACCGGAGCGGGCAGTATCTGCAGAACTCCACGGCAAGCAGCAGTACCGAGTAC
CAGTGTATCCCAGACAGCACCATCCCCCAGGAAGACTACCGCTGCTGGCCATCCTACCACCACGGGAGCT
GCCTCCTTTCAGTGTTCAACCTGGCTGAGGCTGTGGATGTCTGTGAGAGCCATGCCCAGTGTCGGGCCTT
TGTGGTCACCAACCAGACCACCTGGACAGGTCGGCAGCTGGTCTTTTTCAAGACTGGATGGAGCCAAGTG
GTCCCTGATCCCAACAAGACCACATATGTGAAGGCCTCTGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220604 protein sequence
Red=Cloning site Green=Tags(s)

MVLLERLRHPNVLQLYGYCYQDSEDIPDTLTTITELGAPVEMIQLLQTSWEDRFRICLSLGRLLHHLAHS
PLGSVTLLDFRPRQFVLVDGELKVTDLDDARVEETPCAGSTDCILEFPARNFTLPCSAQGWCEGMNEKRN
LYNAYRFFFTYLLPHSAPPSLRPLLDSIVNATGELAWGVDETLAQLEKVLHLYRSGQYLQNSTASSSTEY
QCIPDSTIPQEDYRCWPSYHHGSCLLSVFNLAEAVDVCESHAQCRAFVVTNQTTWTGRQLVFFKTGWSQV
VPDPNKTTYVKASG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138370
ORF Size 882 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138370.1, NP_612379.1
RefSeq Size 2517 bp
RefSeq ORF 1482 bp
Locus ID 91461
UniProt ID Q504Y2
Protein Families Druggable Genome, Protein Kinase
MW 33.3 kDa
Gene Summary Secreted tyrosine-protein kinase that mediates phosphorylation of extracellular proteins and endogenous proteins in the secretory pathway, which is essential for patterning at organogenesis stages. Mediates phosphorylation of MMP1, MMP13, MMP14, MMP19 and ERP29 (PubMed:25171405). Probably plays a role in platelets: rapidly and quantitatively secreted from platelets in response to stimulation of platelet degranulation (PubMed:25171405). May also have serine/threonine protein kinase activity. Required for longitudinal bone growth through regulation of chondrocyte differentiation. May be indirectly involved in protein transport from the Golgi apparatus to the plasma membrane (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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