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FNIP1 (NM_133372) Human Tagged ORF Clone
CAT＃: RC220543
FNIP1 (Myc-DDK-tagged)-Human folliculin interacting protein 1 (FNIP1), transcript variant 1
ORF Plasmid: tGFP
"NM_133372" in other vectors (4)
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CNY 8,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220543 representing NM_133372
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCTACGCTGTTCCAGAAGCTCTTCAGCAAGAGGACCGGGCTGGGCGCGCCCGGCCGCGACGCCC GGGACCCAGATTGCGGGTTCAGTTGGCCTTTACCAGAGTTTGATCCAAGCCAGATTCGACTGATTGTATA TCAAGACTGTGAAAGACGAGGGAGAAATGTTTTGTTTGACTCCAGTGTTAAGAGAAGAAATGAGGACATA TCAGTATCGAAACTCTGCAGTGATGCTCAAGTTAAAGTCTTTGGGAAATGCTGCCAACTGAAACCTGGAG GAGACAGTTCTTCCTCTTTAGATAGTTCTGTGACTTCATCTTCTGATATAAAAGACCAGTGTCTTAAGTA CCAGGGTTCTCGGTGCTCTTCTGATGCCAATATGCTTGGAGAGATGATGTTTGGCTCAGTAGCAATGAGC TACAAAGGATCCACCTTAAAAATTCATCAGATTCGTTCCCCTCCACAGCTCATGCTCAGCAAAGTGTTTA CTGCTCGGACTGGCAGCAGTATTTGTGGGAGTCTCAATACGCTACAAGATAGTCTTGAATTCATCAATCA GGACAACAATACATTAAAGGCTGATAATAACACAGTTATTAATGGACTGCTTGGAAATATAGGTCTTTCA CAGTTCTGCAGCCCCAGGCGGGCATTCTCTGAGCAGGGTCCGCTCCGCCTGATCAGGAGCGCCTCTTTCT TTGCAGTTCACAGCAACCCAATGGACATGCCTGGAAGAGAGCTCAATGAGGACAGAGACAGTGGCATAGC ACGGTCTGCATCTCTCAGCAGCTTGCTGATCACTCCATTTCCTTCCCCAAACTCCTCACTTACCCGAAGT TGTGCCAGCAGCTACCAGCGACGTTGGCGACGCAGCCAAACAACAAGTTTGGAAAATGGGGTATTTCCTA GATGGTCTATAGAAGAAAGCTTTAATCTCTCAGATGAAAGCTGTGGCCCTAACCCAGGAATTGTGCGGAA AAAGAAGATTGCAATTGGGGTAATCTTTTCATTGTCCAAAGATGAAGATGAAAATAACAAATTTAATGAA TTCTTTTTTTCACATTTTCCTCTCTTTGAAAGCCACATGAACAAATTAAAGAGTGCAATAGAACAGGCTA TGAAAATGAGCCGGAGATCAGCTGATGCCAGTCAGAGAAGTTTGGCATATAATCGAATAGTTGATGCCCT AAATGAATTCAGAACAACAATTTGTAATCTTTACACGATGCCACGAATTGGAGAACCTGTCTGGCTTACA ATGATGTCGGGGACTCCAGAAAAGAACCACCTTTGCTATCGTTTCATGAAGGAGTTCACCTTTCTAATGG AAAATGCTTCCAAAAATCAATTCTTGCCAGCTCTCATTACTGCAGTTCTGACCAATCATCTTGCCTGGGT TCCAACAGTCATGCCAAATGGACAACCACCTATAAAAATATTTTTAGAAAAGCATTCCTCTCAGAGTGTG GACATGTTGGCAAAGACTCATCCATATAACCCACTTTGGGCACAACTGGGAGACTTGTATGGCGCTATTG GCTCTCCCGTACGGTTAGCAAGGACTGTGGTAGTTGGCAAACGACAAGACATGGTCCAGAGGCTACTTTA TTTTCTTACTTATTTTATAAGATGCTCTGAACTTCAAGAAACGCATCTTTTAGAAAATGGAGAAGATGAA GCCATCGTTATGCCAGGCACAGTAATTACTACCACTTTAGAGAAAGGTGAAATAGAAGAATCAGAGTATG TCCTTGTCACAATGCATAGAAACAAAAGCAGTTTGCTCTTTAAAGAGTCAGAAGAAATTAGAACTCCCAA TTGTAACTGTAAATATTGCAGTCATCCACTCCTTGGGCAAAATGTAGAGAACATTTCACAACAAGAGAGA GAAGATATTCAAAACAGCTCTAAGGAGCTGCTAGGAATTTCAGATGAGTGCCAGATGATTTCTCCTTCTG ACTGCCAAGAAGAAAATGCTGTTGATGTTAAACAGTACAGAGATAAATTAAGAACTTGCTTTGACGCCAA GTTAGAGACAGTTGTTTGCACAGGATCTGTTCCAGTAGACAAATGTGCATTGTCAGAGTCAGGCTTAGAG TCAACAGAGGAAACATGGCAGAGTGAGAAGTTGCTGGATTCAGACAGTCACACAGGCAAAGCAATGAGAT CCACAGGAATGGTTGTGGAAAAAAAACCTCCAGATAAGATTGTGCCTGCTTCATTTTCTTGTGAGGCTGC CCAGACAAAGGTTACTTTCCTGATTGGGGATTCTATGTCACCTGATTCAGATACTGAGCTTCGAAGTCAG GCAGTGGTGGATCAGATTACCAGACATCACACCAAACCATTGAAGGAAGAAAGAGGGGCTATTGATCAGC ATCAAGAAACTAAACAAACAACCAAGGACCAATCTGGAGAGTCTGATACACAGAACATGGTTTCTGAAGA GCCCTGTGAACTTCCCTGTTGGAATCATTCAGACCCAGAAAGCATGAGCTTATTCGACGAATATTTTAAT GATGATTCAATCGAAACCAGGACTATTGATGATGTTCCATTTAAAACAAGTACAGATAGTAAAGACCATT GCTGTATGTTAGAGTTTTCAAAAATATTGTGTACAAAAAATAACAAGCAGAACAATGAATTTTGTAAATG TATAGAAACAGTTCCCCAAGATTCATGTAAAACCTGCTTTCCTCAGCAGGACCAAAGAGATACACTCTCC ATTCTTGTCCCCCATGGGGATAAAGAGAGTTCAGATAAAAAAATTGCTGTAGGAACTGAATGGGACATTC CAAGAAATGAAAGTTCAGACAGTGCCCTTGGGGATAGTGAAAGTGAAGATACAGGTCATGATATGACTAG ACAAGTTAGCAGTTATTATGGAGGAGAGCAAGAAGATTGGGCAGAAGAGGATGAGATACCTTTTCCTGGG TCAAAGTTAATCGAAGTGAGTGCTGTTCAGCCCAACATTGCCAACTTCGGGAGGTCCTTGCTGGGTGGCT ACTGCTCATCTTATGTGCCTGACTTTGTTCTTCAAGGAATTGGGAGTGATGAGAGGTTCCGTCAGTGTCT GATGTCAGATTTATCTCATGCTGTGCAGCATCCAGTTTTGGATGAACCAATAGCAGAAGCTGTCTGTATT ATAGCTGACATGGATAAATGGACTGTTCAAGTGGCCAGTAGCCAGAGACGAGTGACAGATAATAAATTGG GAAAGGAAGTATTGGTTTCCAGTCTTGTTTCCAATCTGCTTCATTCCACACTTCAGCTTTATAAGCATAA CTTGTCTCCAAATTTTTGTGTAATGCATCTTGAAGACCGGTTGCAGGAGCTATACTTCAAAAGTAAAATG CTGTCTGAATACCTGAGGGGGCAGATGCGTGTTCATGTCAAGGAGCTGGGAGTGGTTCTGGGGATTGAAT CCAGTGATCTTCCACTTCTGGCTGCTGTAGCAAGCACTCACTCTCCATATGTTGCACAAATACTCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220543 representing NM_133372
Red=Cloning site Green=Tags(s) MAPTLFQKLFSKRTGLGAPGRDARDPDCGFSWPLPEFDPSQIRLIVYQDCERRGRNVLFDSSVKRRNEDI SVSKLCSDAQVKVFGKCCQLKPGGDSSSSLDSSVTSSSDIKDQCLKYQGSRCSSDANMLGEMMFGSVAMS YKGSTLKIHQIRSPPQLMLSKVFTARTGSSICGSLNTLQDSLEFINQDNNTLKADNNTVINGLLGNIGLS QFCSPRRAFSEQGPLRLIRSASFFAVHSNPMDMPGRELNEDRDSGIARSASLSSLLITPFPSPNSSLTRS CASSYQRRWRRSQTTSLENGVFPRWSIEESFNLSDESCGPNPGIVRKKKIAIGVIFSLSKDEDENNKFNE FFFSHFPLFESHMNKLKSAIEQAMKMSRRSADASQRSLAYNRIVDALNEFRTTICNLYTMPRIGEPVWLT MMSGTPEKNHLCYRFMKEFTFLMENASKNQFLPALITAVLTNHLAWVPTVMPNGQPPIKIFLEKHSSQSV DMLAKTHPYNPLWAQLGDLYGAIGSPVRLARTVVVGKRQDMVQRLLYFLTYFIRCSELQETHLLENGEDE AIVMPGTVITTTLEKGEIEESEYVLVTMHRNKSSLLFKESEEIRTPNCNCKYCSHPLLGQNVENISQQER EDIQNSSKELLGISDECQMISPSDCQEENAVDVKQYRDKLRTCFDAKLETVVCTGSVPVDKCALSESGLE STEETWQSEKLLDSDSHTGKAMRSTGMVVEKKPPDKIVPASFSCEAAQTKVTFLIGDSMSPDSDTELRSQ AVVDQITRHHTKPLKEERGAIDQHQETKQTTKDQSGESDTQNMVSEEPCELPCWNHSDPESMSLFDEYFN DDSIETRTIDDVPFKTSTDSKDHCCMLEFSKILCTKNNKQNNEFCKCIETVPQDSCKTCFPQQDQRDTLS ILVPHGDKESSDKKIAVGTEWDIPRNESSDSALGDSESEDTGHDMTRQVSSYYGGEQEDWAEEDEIPFPG SKLIEVSAVQPNIANFGRSLLGGYCSSYVPDFVLQGIGSDERFRQCLMSDLSHAVQHPVLDEPIAEAVCI IADMDKWTVQVASSQRRVTDNKLGKEVLVSSLVSNLLHSTLQLYKHNLSPNFCVMHLEDRLQELYFKSKM LSEYLRGQMRVHVKELGVVLGIESSDLPLLAAVASTHSPYVAQILL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133372 |
| ORF Size | 3498 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133372.2, NP_588613.2 |
| RefSeq Size | 6584 bp |
| RefSeq ORF | 3501 bp |
| Locus ID | 96459 |
| UniProt ID | Q8TF40 |
| MW | 130.4 kDa |
| Gene Summary | This gene encodes a protein that binds to the tumor suppressor protein folliculin and to AMP-activated protein kinase (AMPK). The encoded protein participates in the regulation of cellular metabolism and nutrient sensing by modulating the AMPK and target of rapamycin signaling pathways. This gene has a closely related paralog that encodes a protein with similar binding activities. Both related proteins also associate with the molecular chaperone heat shock protein-90 (Hsp90) and negatively regulate its ATPase activity and facilitate its association with folliculin. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220543L3 | Lenti ORF clone of Human folliculin interacting protein 1 (FNIP1), transcript variant 1, Myc-DDK-tagged |
CNY 13,490.00 |
|
| RC220543L4 | Lenti ORF clone of Human folliculin interacting protein 1 (FNIP1), transcript variant 1, mGFP tagged |
CNY 13,490.00 |
|
| RG220543 | FNIP1 (tGFP-tagged) - Human folliculin interacting protein 1 (FNIP1), transcript variant 1 |
CNY 12,730.00 |
|
| SC305948 | FNIP1 (untagged)-Human folliculin interacting protein 1 (FNIP1), transcript variant 1 |
CNY 22,420.00 |
