HECTD3 (NM_024602) Human Tagged ORF Clone
CAT#: RC220357
HECTD3 (Myc-DDK-tagged)-Human HECT domain containing 3 (HECTD3)
ORF Plasmid: tGFP
"NM_024602" in other vectors (7)
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CNY 6,304.00
CNY 9,120.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220357 representing NM_024602
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTCCTGGCCCGGGCGCGGTGCTGGAGTCCCCCCGGCAGCTGCTGGGCCGCGTGCGCTTCTTGG CAGAGGCAGCGCGGAGCCTCCGCGCCGGGCGGCCGCTGCCAGCAGCGCTGGCTTTCGTGCCGCGAGAGGT GCTCTACAAGCTTTACAAGGACCCAGCGGGACCGTCGCGCGTGCTTCTGCCGGTGTGGGAGGCAGAGGGC CTGGGGCTGCGTGTGGGCGCCGCAGGCCCAGCCCCCGGTACCGGCTCCGGGCCCCTCCGCGCCGCCCGCG ACAGCATTGAGCTCCGGCGCGGCGCCTGCGTGCGCACCACGGGCGAGGAGCTGTGCAATGGCCACGGGCT CTGGGTGAAGCTGACAAAGGAGCAGCTGGCAGAGCACCTGGGCGACTGCGGGCTGCAGGAAGGCTGGCTG CTGGTGTGCCGCCCGGCGGAGGGCGGAGCCCGCCTGGTACCCATCGACACTCCCAACCACCTCCAGCGGC AGCAGCAGCTCTTTGGCGTGGATTATCGGCCGGTGCTCAGGTGGGAACAGGTGGTGGACCTGACATACTC ACATCGCCTGGGATCGAGACCTCAGCCGGCAGAGGCATACGCAGAAGCTGTACAAAGGCTACTCTATGTA CCCCCGACATGGACCTACGAGTGCGACGAGGACCTGATCCACTTCTTGTATGACCACCTGGGCAAGGAGG ATGAGAACCTGGGTAGCGTGAAGCAGTATGTGGAGAGCATAGACGTTTCCTCCTACACGGAGGAGTTCAA CGTGTCCTGCCTGACAGACAGCAATGCCGATACCTACTGGGAGAGCGATGGGTCCCAGTGCCAACACTGG GTACGGCTTACTATGAAGAAGGGCACCATTGTCAAGAAGCTGCTACTCACAGTGGATACCACAGATGACA ACTTTATGCCAAAGCGGGTGGTGGTCTATGGGGGTGAAGGGGACAACCTGAAGAAGCTGAGTGACGTGAG CATTGACGAGACCCTCATCGGGGATGTCTGTGTCCTGGAGGACATGACCGTCCACCTCCCGATCATCGAG ATCCGCATCGTGGAGTGCCGAGATGATGGGATTGATGTTCGTCTCCGAGGGGTCAAGATCAAGTCATCTA GACAGCGGGAACTAGGGTTGAATGCAGACCTGTTCCAGCCAACTAGTCTGGTGCGATATCCACGCCTAGA AGGCACCGACCCTGAAGTACTGTACCGCAGAGCTGTCCTCCTGCAGAGATTCATCAAGATCCTCGATAGT GTCCTGCACCACCTGGTACCTGCCTGGGACCACACACTGGGCACCTTCAGTGAGATTAAGCAAGTGAAGC AGTTCCTACTGCTGTCCCGCCAGCGGCCAGGCCTGGTGGCTCAGTGCCTGCGTGACTCTGAGAGCAGCAA GCCCAGCTTCATGCCACGCCTATACATCAACCGCCGTCTTGCCATGGAACACCGTGCCTGCCCCTCTCGA GACCCTGCCTGCAAGAATGCAGTCTTCACCCAGGTATATGAAGGCCTCAAGCCCTCTGACAAATATGAAA AGCCCCTGGACTACAGGTGGCCCATGCGCTATGACCAGTGGTGGGAGTGTAAATTTATTGCAGAAGGCAT CATTGACCAAGGGGGTGGTTTCCGGGACAGCCTGGCAGATATGTCAGAAGAGCTGTGCCCTAGCTCAGCG GATACCCCCGTGCCCCTGCCCTTCTTTGTACGCACAGCCAACCAGGGCAATGGCACTGGTGAGGCTCGGG ACATGTATGTACCCAACCCCTCCTGCCGAGACTTTGCCAAGTATGAATGGATCGGACAGCTGATGGGGGC TGCCCTTCGGGGTAAGGAGTTCCTGGTCCTGGCCCTGCCTGGTTTTGTGTGGAAGCAGCTTTCTGGTGAG GAGGTGAGCTGGAGCAAGGACTTCCCAGCTGTGGACTCTGTGCTGGTGAAGCTCCTGGAAGTGATGGAAG GAATGGACAAGGAGACGTTTGAGTTCAAGTTTGGGAAGGAACTAACATTCACCACTGTACTGAGTGACCA ACAGGTGGTGGAGCTGATCCCTGGGGGTGCAGGCATCGTCGTGGGATATGGGGACCGTTCTCGTTTCATC CAACTGGTCCAGAAGGCACGGCTAGAGGAGAGCAAGGAGCAGGTGGCAGCTATGCAGGCAGGTCTGCTGA AGGTGGTACCACAGGCTGTGCTGGACTTGCTGACCTGGCAAGAGTTGGAGAAGAAAGTGTGTGGGGATCC AGAGGTCACTGTGGATGCTCTGCGCAAGCTCACCCGGTTTGAGGACTTCGAGCCATCTGACTCGCGGGTG CAGTATTTCTGGGAGGCACTGAACAACTTCACCAACGAGGACCGGAGCCGCTTCCTGCGCTTTGTCACGG GCCGCAGTCGCCTGCCAGCACGGATCTACATCTACCCAGACAAGCTGGGCTACGAGACCACAGACGCGCT GCCCGAGTCTTCCACTTGCTCCAGCACCCTCTTCCTGCCACACTATGCCAGTGCCAAGGTATGCGAGGAG AAGCTCCGCTATGCGGCCTACAACTGCGTGGCCATCGACACTGACATGAGCCCTTGGGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220357 representing NM_024602
Red=Cloning site Green=Tags(s) MAGPGPGAVLESPRQLLGRVRFLAEAARSLRAGRPLPAALAFVPREVLYKLYKDPAGPSRVLLPVWEAEG LGLRVGAAGPAPGTGSGPLRAARDSIELRRGACVRTTGEELCNGHGLWVKLTKEQLAEHLGDCGLQEGWL LVCRPAEGGARLVPIDTPNHLQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYAEAVQRLLYV PPTWTYECDEDLIHFLYDHLGKEDENLGSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQCQHW VRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIE IRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLEGTDPEVLYRRAVLLQRFIKILDS VLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSR DPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSA DTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGE EVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFI QLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRV QYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEE KLRYAAYNCVAIDTDMSPWEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024602 |
ORF Size | 2583 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024602.6 |
RefSeq Size | 3628 bp |
RefSeq ORF | 2586 bp |
Locus ID | 79654 |
UniProt ID | Q5T447 |
Domains | HECT |
Protein Families | Druggable Genome |
MW | 97.6 kDa |
Gene Summary | The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220357L1 | Lenti ORF clone of Human HECT domain containing 3 (HECTD3), Myc-DDK-tagged |
CNY 8,704.00 |
|
RC220357L2 | Lenti ORF clone of Human HECT domain containing 3 (HECTD3), mGFP tagged |
CNY 11,020.00 |
|
RC220357L3 | Lenti ORF clone of Human HECT domain containing 3 (HECTD3), Myc-DDK-tagged |
CNY 11,020.00 |
|
RC220357L4 | Lenti ORF clone of Human HECT domain containing 3 (HECTD3), mGFP tagged |
CNY 11,020.00 |
|
RG220357 | HECTD3 (tGFP-tagged) - Human HECT domain containing 3 (HECTD3) |
CNY 7,904.00 |
|
SC112112 | HECTD3 (untagged)-Human HECT domain containing 3 (HECTD3) |
CNY 2,400.00 |
|
SC318338 | HECTD3 (untagged)-Human HECT domain containing 3 (HECTD3) |
CNY 16,630.00 |