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GCM1 (NM_003643) Human Tagged ORF Clone
CAT＃: RC220319
GCM1 (Myc-DDK-tagged)-Human glial cells missing homolog 1 (Drosophila) (GCM1)
ORF Plasmid: tGFP
"NM_003643" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GCMA; hGCMa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220319 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCTGACGACTTTGATTCTGAAGACAAAGAGATATTAAGCTGGGATATTAATGATGTGAAACTGC CACAGAACGTGAAAAAAACCGACTGGTTCCAGGAGTGGCCAGATTCCTATGCCAAACACATCTACAGCTC GGAGGACAAGAATGCGCAGCGGCACCTGAGCAGCTGGGCCATGCGCAATACCAACAACCACAACTCCCGC ATCCTCAAGAAGTCCTGCCTGGGTGTGGTGGTGTGCGGCCGCGACTGTCTCGCAGAGGAGGGGCGCAAGA TCTACCTGAGACCTGCCATCTGTGACAAGGCCCGGCAGAAGCAGCAGCGGAAACGCTGTCCCAACTGTGA CGGGCCTCTGAAGCTCATCCCTTGCCGAGGTCATGGGGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC GGACGCTTTATATTTTTCCAGTCAAAGGGAGAGCATGATCATCCAAAACCAGAAACCAAGTTAGAAGCTG AGGCAAGAAGAGCCATGAAGAAAGTGAACACAGCACCTTCCTCCGTCTCATTGAGCCTGAAGGGGAGCAC AGAGACCAGGTCTCTTCCAGGTGAAACACAAAGTCAGGGGAGTTTACCTTTAACTTGGTCTTTCCAGGAA GGCGTCCAATTGCCTGGTAGTTACAGTGGACATTTAATAGCTAACACTCCTCAGCAGAACTCACTAAATG ATTGCTTTTCCTTCTCCAAGAGTTATGGTCTGGGAGGAATCACAGATCTGACTGACCAGACTTCCACTGT GGACCCCATGAAGCTCTATGAAAAGCGCAAATTGTCCAGTAGCAGAACCTACAGTAGTGGAGACCTGCTT CCTCCTTCTGCCTCCGGAGTCTACTCTGATCATGGCGATCTACAAGCGTGGAGTAAAAATGCTGCTTTGG GGAGAAATCATCTTGCTGACAACTGTTATTCCAATTATCCTTTTCCTCTGACCAGCTGGCCTTGCAGCTT CTCTCCTTCCCAAAACTCTTCAGAACCCTTTTACCAGCAGCTTCCATTGGAGCCACCTGCAGCCAAAACT GGCTGTCCCCCATTATGGCCAAATCCAGCGGGTAATCTTTATGAAGAGAAAGTACATGTGGATTTTAACA GCTACGTCCAGTCTCCTGCATACCATTCACCTCAAGAAGACCCCTTTCTCTTCACCTACGCCTCTCATCC TCATCAGCAATATTCACTGCCAAGCAAGAGCAGCAAATGGGATTTTGAGGAAGAAATGACATACTTGGGT TTGGATCACTGCAACAATGATATGCTTCTGAACCTGTGTCCTTTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220319 protein sequence
Red=Cloning site Green=Tags(s) MEPDDFDSEDKEILSWDINDVKLPQNVKKTDWFQEWPDSYAKHIYSSEDKNAQRHLSSWAMRNTNNHNSR ILKKSCLGVVVCGRDCLAEEGRKIYLRPAICDKARQKQQRKRCPNCDGPLKLIPCRGHGGFPVTNFWRHD GRFIFFQSKGEHDHPKPETKLEAEARRAMKKVNTAPSSVSLSLKGSTETRSLPGETQSQGSLPLTWSFQE GVQLPGSYSGHLIANTPQQNSLNDCFSFSKSYGLGGITDLTDQTSTVDPMKLYEKRKLSSSRTYSSGDLL PPSASGVYSDHGDLQAWSKNAALGRNHLADNCYSNYPFPLTSWPCSFSPSQNSSEPFYQQLPLEPPAAKT GCPPLWPNPAGNLYEEKVHVDFNSYVQSPAYHSPQEDPFLFTYASHPHQQYSLPSKSSKWDFEEEMTYLG LDHCNNDMLLNLCPLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003643 |
| ORF Size | 1308 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003643.4 |
| RefSeq Size | 2780 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 8521 |
| UniProt ID | Q9NP62 |
| Domains | GCM |
| Protein Families | Transcription Factors |
| MW | 49.3 kDa |
| Gene Summary | This gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220319L1 | Lenti ORF clone of Human glial cells missing homolog 1 (Drosophila) (GCM1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC220319L2 | Lenti ORF clone of Human glial cells missing homolog 1 (Drosophila) (GCM1), mGFP tagged |
CNY 5,890.00 |
|
| RC220319L3 | Lenti ORF clone of Human glial cells missing homolog 1 (Drosophila) (GCM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220319L4 | Lenti ORF clone of Human glial cells missing homolog 1 (Drosophila) (GCM1), mGFP tagged |
CNY 5,890.00 |
|
| RG220319 | GCM1 (tGFP-tagged) - Human glial cells missing homolog 1 (Drosophila) (GCM1) |
CNY 5,256.00 |
|
| SC313458 | GCM1 (untagged)-Human glial cells missing homolog 1 (Drosophila) (GCM1) |
CNY 3,656.00 |
