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CD300E (NM_181449) Human Tagged ORF Clone
CAT＃: RC220200
CD300E (Myc-DDK-tagged)-Human CD300e molecule (CD300E)
ORF Plasmid: tGFP
"NM_181449" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD300LE; CLM-2; CLM2; CMRF35-A5; IREM-2; IREM2; PIgR-2; PIgR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220200 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGCTCCCAGCTCTACTCCTTCTCTGCCTCTCAGGCTGTTTGTCTCTGAAGGGCCCCGGCTCTG TGACTGGCACTGCGGGGGACTCTCTGACAGTGTGGTGTCAGTATGAGAGCATGTACAAGGGATATAACAA GTACTGGTGCCGAGGACAGTACGACACGTCATGTGAGAGCATTGTGGAGACCAAGGGAGAAGAGAAGGTG GAGAGGAATGGCCGCGTGTCCATCAGAGACCACCCGGAGGCTCTCGCCTTCACTGTGACCATGCAGAACC TCAATGAAGATGATGCTGGATCTTACTGGTGCAAAATTCAGACAGTGTGGGTCCTGGATTCATGGTCACG CGATCCCTCGGACCTGGTTAGGGTGTATGTTTCCCCAGCAATTACAACCCCAAGGAGGACCACACATCCA GCCACACCTCCCATCTTCCTGGTGGTGAACCCTGGGCGAAACCTCAGCACCAGGGAGGTGTTGACCCAAA ATTCAGGGTTCCGGCTCAGCAGCCCTCACTTCCTGCTCGTGGTCCTTCTGAAGCTGCCCCTGCTCCTGAG CATGCTGGGTGCTGTTTTCTGGGTGAACAGGCCTCAGTGGGCTCCTCCTGGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220200 protein sequence
Red=Cloning site Green=Tags(s) MWLLPALLLLCLSGCLSLKGPGSVTGTAGDSLTVWCQYESMYKGYNKYWCRGQYDTSCESIVETKGEEKV ERNGRVSIRDHPEALAFTVTMQNLNEDDAGSYWCKIQTVWVLDSWSRDPSDLVRVYVSPAITTPRRTTHP ATPPIFLVVNPGRNLSTREVLTQNSGFRLSSPHFLLVVLLKLPLLLSMLGAVFWVNRPQWAPPGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181449 |
| ORF Size | 615 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181449.3 |
| RefSeq Size | 3525 bp |
| RefSeq ORF | 618 bp |
| Locus ID | 342510 |
| UniProt ID | Q496F6 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 23 kDa |
| Gene Summary | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins expressed on myeloid cells. The protein interacts with the TYRO protein tyrosine kinase-binding protein and is thought to act as an activating receptor. [provided by RefSeq, Nov 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220200L3 | Lenti ORF clone of Human CD300e molecule (CD300E), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220200L4 | Lenti ORF clone of Human CD300e molecule (CD300E), mGFP tagged |
CNY 5,890.00 |
|
| RG220200 | CD300E (tGFP-tagged) - Human CD300e molecule (CD300E) |
CNY 4,000.00 |
|
| SC107220 | CD300E (untagged)-Human CD300e molecule (CD300E) |
CNY 2,400.00 |
