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KIAA0586 (NM_014749) Human Tagged ORF Clone
CAT＃: RC220179
- TrueORF®
KIAA0586 (Myc-DDK-tagged)-Human KIAA0586 (KIAA0586)
ORF Plasmid: tGFP
"NM_014749" in other vectors (4)
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CNY 10,072.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JBTS23; SRTD14; Talpid3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220179 representing NM_014749
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGCTCTGAGGTCAGCTTGGAGAAGAAAAAAAAGATTAAGATGCCAGTGAAGAGACTTCGTGAGG TAGTTTCTCAAAATCATGGAGATCATTTGGTTTTGCTGAAAGATGAGTTGCCCTGTGTTCCTCCGGCATT GTCTGCAAATAAACGTCTTCCTGTTGGAACGGGGACTAGTTTGAATGGAACATCACGTGGTTCATCAGAC TTAACTTCTGCTAGAAATTGTTACCAGCCTCTATTAGAAAATCCCATGGTGTCAGAAAGTGATTTTTCTA AAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAAAATAGAAGAGAACAACAAGCAAAAAGCAAATGACAT CTTCATTTCTCAGTATACAATGGGACAGAAAGATGCTCTAAGAACAGTTTTAAAGCAAAAAGCTCAAAGC ATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCAGGCATAGAGAAGGATGCTGTTACTC AGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCGTGGCTGCAGCAACTGCTGCTGCCAT TGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGCAAAAGTCAATTCTGTTACAGAATTA CTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACAGAGCAGCAAACAAGCATTCAGAGGA AACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATGTGTTTATGGAGCAGCACATAAGGCA TCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTTTATTAGTGCTGCACTCAAGACTAGT AGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAGTATTCCGTAAAACCAGAACACCCTA ATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCAAACAAGCACCTTTAAAAGAAGTTGA AGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGCACCTCGCAGATTTGCTCCTGTACCT GTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAAGAAAAAGAAAATATGGAAGTGTCGT GTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATAATGATTCTTTGACAAGAAAAAGTGA ATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGACTCCTGAGAAAACAAACAGATTTCCT TCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCTGATGATGTTCTTCATGACCTTGGCC AAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCTCTGAGTATGTTGAAGCTTCCAGATCT TCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGATCTGTATTGAAAGATGCTGAGAAGATT TTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAACCTGGAAGCTATTATTCGTGCAAAAGATG GAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAACAGAGAGATGTCAGAGAAAATTAGGATCAG AAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAAATTCAGGGGCTTTTGAAAGCAACCACAGTA ATACAAGATGAAGATTATATGTTACAAGTCTATGGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTTA AAAAAGGACCATATCTCAGATTTAATTCTCCATCTCCTAAGTCCAGACCACAGAGACCAAAAGTAATAGA ACGAGTTAAAGGCACTAAGGTAAAGTCAATAAGAACACAGACTGACTTCTATGCAACAAAACCTAAGAAG ATGGATTCTAAAATGAAACATTCTGTTCCTGTGTTACCTCATGGCGATCAGCAATATTTGTTCAGCCCAA GTAGAGAAATGCCTACTTTTTCAGGTACATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTAGGACA AACCCAAAGTAATAGTGATACCATGCCACCTGCTGGAGTGATTGTCAGCAAGCCACACCCTGTAACTGTG ACTACTTCTATTCCTCCATCATCTCGAAAAGTAGAAACTGGAGTAAAGAAACCTAACATAGCCATTGTAG AAATGAAGTCAGAAAAAAAGGATCCTCCTCAGCTTACTGTGCAGGTATTACCCAGTGTAGATATTGACAG CATTTCAAATAGTAGTGCTGATGTCCTTTCACCTCTGTCTAGCCCCAAAGAAGCATCTCTTCCTCCTGTG CAAACTTGGATAAAGACTCCAGAAATTATGAAGGTAGATGAAGAAGAGGTGAAGTTTCCAGGAACTAACT TTGATGAAATAATCGATGTCATACAGGAAGAAGAAAAATGTGATGAAATTCCAGACTCTGAACCAATTCT GGAGTTTAACAGAAGTGTTAAAGCTGATTCTACAAAATATAATGGTCCTCCATTTCCGCCAGTTGCTTCT ACTTTTCAGCCCACTGCTGATATTCTGGATAAAGTAATTGAGAGAAAAGAAACACTGGAAAATAGCTTAA TTCAATGGGTAGAGCAAGAAATAATGTCAAGAATTATCTCTGGGCTCTTTCCAGTCCAGCAACAGATTGC ACCTAGTATCAGTGTTTCAGTCAGTGAGACAAGTGAACCACTGACTTCTGACATTGTGGAAGGAACAAGC AGTGGCGCCCTCCAGCTTTTTGTTGATGCTGGTGTTCCTGTGAACTCAAATGTGATTAAACATTTTGTTA ACGAAGCTCTTGCTGAGACCATTGCTGTCATGCTGGGTGACAGAGAAGCAAAGAAGCAAGGTCCTGTTGC TACAGGTGTTTCTGGGGATGCTTCAACAAATGAAACATATTTGCCGGCAAGAGTGTGCACCCCACTGCCT ACCCCACAGCCTACGCCTCCTTGCTCACCTTCATCACCTGCTAAGGAGTGTGTTTTGGTAAAGACTCCAG ATTCTTCTCCCTGTGATTCGGATCATGATATGGCTTTTCCTGTGAAAGAAATATGTGCTGAAAAAGGAGA TGATATGCCTGCCATCATGCTTGTTAATACTCCAACAGTTACCCCTACTACTACACCTCCTCCAGCGGCG GCAGTTTTTACCCCAACTTTGTCAGATATTTCCATTGATAAATTGAAGGTATCAAGCCCAGAGCTTCCCA AGCCATGGGGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCTAACTCACCTCAAGAAGAACTTCATCC AAGAGCTATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGAGTATGGATTTCCCTGCTCAGCCTCCA CCTCCAGAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAAGGCCCCTTCCCCCTCACAGATGCCAG GTTCTGATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTCACTGAAACTGAAACTTTAGATAAACC CATCTCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTGGCCCCCAAGATTTTAGAAGATATAGGA CTGTACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTGCATGATGCCGTTGAAATGGAGGATGATC CTCCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAATTTCATGCAGATGCAATTCTTTCTTTTGC TAAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTCTATCATTCAGAGGACTTGGAAAACAGTGTG GGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCGGCAGCAGAGAACATCTTAATGGGACATTCTC TCTATATGCAGCCACCTGTCACTAATACACAGTCTTTGGATCAACAATGTGATCCTAAACCATTATCTCG GCAATTTGACACAGTTTCAGGTAGTATTTATGAAGATTCATGTGCTAGTCATGGTCCAATGAGTTTGGGA GAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTTCCCACAACACTTCTGACAGCACAAGAAAATGATG TTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAGTACCAACTAAAGCAAAATCAGGATGTTAAGCAAGT TGAACACAAACCATCACAAAGTTACCTACGTGTTAGAAATAAATCTGATATTGCACCTTCACAGCAACAA GTTTCACCAGGTGATATGGATCGGACACAAATTGAGCTTAATCCGTACCTCACATGTGTATTTTCAGGTG GGAAAGCAGTGCCACTCTCCGCTTCACAGATGCCCCCTGCCAAGATGTCAGTGATGCTGCCGTCAGTGAA CCTCGAGGACTGCTCTCAGTCTCTGAGTCTCAGCACAATGCAGGAGGACATGGAGTCTTCGGGGGCAGAT ACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220179 representing NM_014749
Red=Cloning site Green=Tags(s) MKGSEVSLEKKKKIKMPVKRLREVVSQNHGDHLVLLKDELPCVPPALSANKRLPVGTGTSLNGTSRGSSD LTSARNCYQPLLENPMVSESDFSKDVAVQVLPLDKIEENNKQKANDIFISQYTMGQKDALRTVLKQKAQS MPVFKEVKVHLLEDAGIEKDAVTQETRISPSGIDSATTVAAATAAAIATAAPLIKVQSDLEAKVNSVTEL LSKLQETDKHLQRVTEQQTSIQRKQEKLHCHDHEKQMNVFMEQHIRHLEKLQQQQIDIQTHFISAALKTS SFQPVSMPSSRAVEKYSVKPEHPNLGSCNPSLYNTFASKQAPLKEVEDTSFDKQKSPLETPAPRRFAPVP VSRDDELSKRENLLEEKENMEVSCHRGNVRLLEQILNNNDSLTRKSESSNTTSLTRSKIGWTPEKTNRFP SCEELETTKVTMQKSDDVLHDLGQKEKETNSMVQPKESLSMLKLPDLPQNSVKLQTTNTTRSVLKDAEKI LRGVQNNKKVLEENLEAIIRAKDGAAMYSLINALSTNREMSEKIRIRKTVDEWIKTISAEIQGLLKATTV IQDEDYMLQVYGKPVYQGHRSTLKKGPYLRFNSPSPKSRPQRPKVIERVKGTKVKSIRTQTDFYATKPKK MDSKMKHSVPVLPHGDQQYLFSPSREMPTFSGTLEGHLIPMAILLGQTQSNSDTMPPAGVIVSKPHPVTV TTSIPPSSRKVETGVKKPNIAIVEMKSEKKDPPQLTVQVLPSVDIDSISNSSADVLSPLSSPKEASLPPV QTWIKTPEIMKVDEEEVKFPGTNFDEIIDVIQEEEKCDEIPDSEPILEFNRSVKADSTKYNGPPFPPVAS TFQPTADILDKVIERKETLENSLIQWVEQEIMSRIISGLFPVQQQIAPSISVSVSETSEPLTSDIVEGTS SGALQLFVDAGVPVNSNVIKHFVNEALAETIAVMLGDREAKKQGPVATGVSGDASTNETYLPARVCTPLP TPQPTPPCSPSSPAKECVLVKTPDSSPCDSDHDMAFPVKEICAEKGDDMPAIMLVNTPTVTPTTTPPPAA AVFTPTLSDISIDKLKVSSPELPKPWGDGDLPLEEENPNSPQEELHPRAIVMSVAKDEEPESMDFPAQPP PPEPVPFMPFPAGTKAPSPSQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPKILEDIG LYLTNLNDSLSSTLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESAVSQQAVYHSEDLENSV GELSEGQRPQLTAAAENILMGHSLYMQPPVTNTQSLDQQCDPKPLSRQFDTVSGSIYEDSCASHGPMSLG ELELEPNSKLVLPTTLLTAQENDVNLPVAAEDFSQYQLKQNQDVKQVEHKPSQSYLRVRNKSDIAPSQQQ VSPGDMDRTQIELNPYLTCVFSGGKAVPLSASQMPPAKMSVMLPSVNLEDCSQSLSLSTMQEDMESSGAD TF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014749 |
| ORF Size | 4416 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014749.5 |
| RefSeq Size | 4994 bp |
| RefSeq ORF | 4419 bp |
| Locus ID | 9786 |
| UniProt ID | Q9BVV6 |
| MW | 161.7 kDa |
| Gene Summary | This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220179L3 | Lenti-ORF clone of KIAA0586 (Myc-DDK-tagged)-Human KIAA0586 (KIAA0586) |
CNY 16,630.00 |
|
| RC220179L4 | Lenti-ORF clone of KIAA0586 (mGFP-tagged)-Human KIAA0586 (KIAA0586) |
CNY 16,630.00 |
|
| RG220179 | KIAA0586 (tGFP-tagged) - Human KIAA0586 (KIAA0586) |
CNY 16,250.00 |
|
| SC127398 | KIAA0586 (untagged)-Human KIAA0586 (KIAA0586) |
CNY 11,328.00 |
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