TRPV4 (NM_021625) Human Tagged ORF Clone

CAT＃: RC220160

TRPV4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_021625" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 6,376.00

货期*
现货
规格
    • 10 ug
Cited in 3 publications.

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA324060
Anti-TRPV4 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

添加到购物车

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BCYM3; CMT2C; HMSN2C; OTRPC4; SMAL; SPSMA; SSQTL1; TRP12; VRL2; VROAC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220160 representing NM_021625.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGGATTCCAGCGAAGGCCCCCGCGCGGGGCCCGGGGAGGTGGCTGAGCTCCCCGGGGATGAGAGT
GGCACCCCAGGTGGGGAGGCTTTTCCTCTCTCCTCCCTGGCCAATCTGTTTGAGGGGGAGGATGGCTCC
CTTTCGCCCTCACCGGCTGATGCCAGTCGCCCTGCTGGCCCAGGCGATGGGCGACCAAATCTGCGCATG
AAGTTCCAGGGCGCCTTCCGCAAGGGGGTGCCCAACCCCATCGATCTGCTGGAGTCCACCCTATATGAG
TCCTCGGTGGTGCCTGGGCCCAAGAAAGCACCCATGGACTCACTGTTTGACTACGGCACCTATCGTCAC
CACTCCAGTGACAACAAGAGGTGGAGGAAGAAGATCATAGAGAAGCAGCCGCAGAGCCCCAAAGCCCCT
GCCCCTCAGCCGCCCCCCATCCTCAAAGTCTTCAACCGGCCTATCCTCTTTGACATCGTGTCCCGGGGC
TCCACTGCTGACCTGGACGGGCTGCTCCCATTCTTGCTGACCCACAAGAAACGCCTAACTGATGAGGAG
TTTCGAGAGCCATCTACGGGGAAGACCTGCCTGCCCAAGGCCTTGCTGAACCTGAGCAATGGCCGCAAC
GACACCATCCCTGTGCTGCTGGACATCGCGGAGCGCACCGGCAACATGCGGGAGTTCATTAACTCGCCC
TTCCGTGACATCTACTATCGAGGTCAGACAGCCCTGCACATCGCCATTGAGCGTCGCTGCAAACACTAC
GTGGAACTTCTCGTGGCCCAGGGAGCTGATGTCCACGCCCAGGCCCGTGGGCGCTTCTTCCAGCCCAAG
GATGAGGGGGGCTACTTCTACTTTGGGGAGCTGCCCCTGTCGCTGGCTGCCTGCACCAACCAGCCCCAC
ATTGTCAACTACCTGACGGAGAACCCCCACAAGAAGGCGGACATGCGGCGCCAGGACTCGCGAGGCAAC
ACAGTGCTGCATGCGCTGGTGGCCATTGCTGACAACACCCGTGAGAACACCAAGTTTGTTACCAAGATG
TACGACCTGCTGCTGCTCAAGTGTGCCCGCCTCTTCCCCGACAGCAACCTGGAGGCCGTGCTCAACAAC
GACGGCCTCTCGCCCCTCATGATGGCTGCCAAGACGGGCAAGATTGGGATCTTTCAGCACATCATCCGG
CGGGAGGTGACGGATGAGGACACACGGCACCTGTCCCGCAAGTTCAAGGACTGGGCCTATGGGCCAGTG
TATTCCTCGCTTTATGACCTCTCCTCCCTGGACACGTGTGGGGAAGAGGCCTCCGTGCTGGAGATCCTG
GTGTACAACAGCAAGATTGAGAACCGCCACGAGATGCTGGCTGTGGAGCCCATCAATGAACTGCTGCGG
GACAAGTGGCGCAAGTTCGGGGCCGTCTCCTTCTACATCAACGTGGTCTCCTACCTGTGTGCCATGGTC
ATCTTCACTCTCACCGCCTACTACCAGCCGCTGGAGGGCACACCGCCGTACCCTTACCGCACCACGGTG
GACTACCTGCGGCTGGCTGGCGAGGTCATTACGCTCTTCACTGGGGTCCTGTTCTTCTTCACCAACATC
AAAGACTTGTTCATGAAGAAATGCCCTGGAGTGAATTCTCTCTTCATTGATGGCTCCTTCCAGCTGCTC
TACTTCATCTACTCTGTCCTGGTGATCGTCTCAGCAGCCCTCTACCTGGCAGGGATCGAGGCCTACCTG
GCCGTGATGGTCTTTGCCCTGGTCCTGGGCTGGATGAATGCCCTTTACTTCACCCGTGGGCTGAAGCTG
ACGGGGACCTATAGCATCATGATCCAGAAGATTCTCTTCAAGGACCTTTTCCGATTCCTGCTCGTCTAC
TTGCTCTTCATGATCGGCTACGCTTCAGCCCTGGTCTCCCTCCTGAACCCGTGTGCCAACATGAAGGTG
TGCAATGAGGACCAGACCAACTGCACAGTGCCCACTTACCCCTCGTGCCGTGACAGCGAGACCTTCAGC
ACCTTCCTCCTGGACCTGTTTAAGCTGACCATCGGCATGGGCGACCTGGAGATGCTGAGCAGCACCAAG
TACCCCGTGGTCTTCATCATCCTGCTGGTGACCTACATCATCCTCACCTTTGTGCTGCTCCTCAACATG
CTCATTGCCCTCATGGGCGAGACAGTGGGCCAGGTCTCCAAGGAGAGCAAGCACATCTGGAAGCTGCAG
TGGGCCACCACCATCCTGGACATTGAGCGCTCCTTCCCCGTATTCCTGAGGAAGGCCTTCCGCTCTGGG
GAGATGGTCACCGTGGGCAAGAGCTCGGACGGCACTCCTGACCGCAGGTGGTGCTTCAGGGTGGATGAG
GTGAACTGGTCTCACTGGAACCAGAACTTGGGCATCATCAACGAGGACCCGGGCAAGAATGAGACCTAC
CAGTATTATGGCTTCTCGCATACCGTGGGCCGCCTCCGCAGGGATCGCTGGTCCTCGGTGGTACCCCGC
GTGGTGGAACTGAACAAGAACTCGAACCCGGACGAGGTGGTGGTGCCTCTGGACAGCATGGGGAACCCC
CGCTGCGATGGCCACCAGCAGGGTTACCCCCGCAAGTGGAGGACTGATGACGCCCCGCTC
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
>Peptide sequence encoded by RC220160
Blue=ORF Red=Cloning site Green=Tag(s)

MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRM
KFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAP
APQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRN
DTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPK
DEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKM
YDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPV
YSSLYDLSSLDTCGEEASVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMV
IFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTNIKDLFMKKCPGVNSLFIDGSFQLL
YFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVY
LLFMIGYASALVSLLNPCANMKVCNEDQTNCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTK
YPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSG
EMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPR
VVELNKNSNPDEVVVPLDSMGNPRCDGHQQGYPRKWRTDDAPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021625
ORF Size 2613 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021625.3, NP_067638.3
RefSeq Size 3254 bp
RefSeq ORF 2616 bp
Locus ID 59341
UniProt ID Q9HBA0
Domains ANK, ion_trans
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW 98.3 kDa
Gene Summary This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

The use of this cDNA Clones has been cited in the following citations:
TRPV4 and KRAS and FGFR1 gain-of-function mutations drive giant cell lesions of the jaw ,Gomes, CC;Gayden, T;Bajic, A;Harraz, OF;Pratt, J;Nikbakht, H;Bareke, E;Diniz, MG;Castro, WH;St-Onge, P;Sinnett, D;Han, H;Rivera, B;Mikael, LG;De Jay, N;Kleinman, CL;Valera, ET;Bassenden, AV;Berghuis, AM;Majewski, J;Nelson, MT;Gomez, RS;Jabado, N;, Nat Commun ,PubMed ID 30385747 [TRPV4]
Transient receptor potential vanilloid 4 (TRPV4) activation by arachidonic acid requires protein kinase A-mediated phosphorylation ,Cao, S;Anishkin, A;Zinkevich, NS;Nishijima, Y;Korishettar, A;Wang, Z;Fang, J;Wilcox, DA;Zhang, DX;, J. Biol. Chem. ,PubMed ID 29462784 [TRPV4]
Arachidonic Acid–Induced Dilation in Human Coronary Arterioles: Convergence of Signaling Mechanisms on Endothelial TRPV4‐Mediated Ca 2+ Entry ,null, Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease ,PubMed ID 23619744 [TRPV4]

Other Versions

SKU Description Size Price
RC220160L1 Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, Myc-DDK-tagged
    • 10 ug

CNY 8,776.00

RC220160L2 Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, mGFP tagged
    • 10 ug

CNY 5,890.00

RC220160L3 Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, Myc-DDK-tagged
    • 10 ug

CNY 8,776.00

RC220160L4 Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, mGFP tagged
    • 10 ug

CNY 8,776.00

RG220160 TRPV4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1
    • 10 ug

CNY 7,976.00

SC309684 TRPV4 (untagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1
    • 10 ug

CNY 6,384.00
CNY 7,220.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它TRPV4产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (13)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

    • 10 ug

CNY 5,420.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

CNY 4,090.00

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

    • 100 ul

CNY 1,050.00

RC220160L1

Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, Myc-DDK-tagged

    • 10 ug

CNY 8,776.00

RC220160L2V

Lenti ORF particles, TRPV4 (mGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 9,975.00 暂停销售

RC220160L4

Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, mGFP tagged

    • 10 ug

CNY 8,776.00

RC220160L2

Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, mGFP tagged

    • 10 ug

CNY 5,890.00

RC220160L3V

Lenti ORF particles, TRPV4 (Myc-DDK tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 8,930.00 暂停销售

RC220160L1V

Lenti ORF particles, TRPV4 (Myc-DDK tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 8,930.00 暂停销售

SC309684

TRPV4 (untagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1

    • 10 ug

CNY 6,384.00 CNY 7,220.00

RC220160L4V

Lenti ORF particles, TRPV4 (mGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 9,975.00 暂停销售

RG220160

TRPV4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1

    • 10 ug

CNY 7,976.00

RC220160L3

Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, Myc-DDK-tagged

    • 10 ug

CNY 8,776.00

Previous
Next
Customer Reviews