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ALPK3 (NM_020778) Human Tagged ORF Clone
CAT＃: RC220076
- TrueORF®
ALPK3 (Myc-DDK-tagged)-Human alpha-kinase 3 (ALPK3)
ORF Plasmid: tGFP
"NM_020778" in other vectors (3)
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CNY 19,224.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMH27; MAK; MIDORI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220076 representing NM_020778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGCTTGGCTTGTCTATGTGCTGGGCCAACAGCCACTGGCGAGGCAAGGCGAGGGTCAGTCAC GGCTGGTGCCAGGAAGAGGGCTGGTTCTTTGGCTCCCTGGTCTCCCGCGGTCTAGCCCAAGCTGGCCAGC GGTTGACCTGGCTCCCCTGGCCCCGGCCAGGCCTCGTGGACCCCTCATATGCCACACGGGACATGAGCAG GCCGGCCGGGAGCCGGGTCCCGGGAGCTCCACGAAGGGGCCTGTCCTCCATGACCAGGACACCCGCTGCG CCTTCCTCCCGAGGCCTCCCGGGCCTCTCCAGACGCGGCGCTACTGCAGACACCAGGGCCGCCAAGGGAG CGGACTCGGAGCCGGCCCTGGGGCGGGCACATGGGCCCCGGCGCCCCCCGGCGTCTCCAAGCCGCGCTGC CCGGGTCGGGCCAGGCCAGGGGAGGGACAGCAGCAGGTGACGACGGCCCGGCCACCGGCTATAAATAGGG GCGCGCGTCAGCCGCGGGCGGGAGCGGCGGCGGCGGGCAGGGGCCCGGGGGCCGGGGCCTGGAGGACAGG CGAGGCAGCGGCGAGTGCGGGGCCGGCGGTCGGGGAGGGCGGTGCCATGGGGTCGCGGAGGGCCCCCACC CGGGGCTGGGGCGCGGGTGGGCGGTCGGGGGCGGGGGGCGACGGTGAGGACGACGGCCCCGTGTGGATCC CCAGCCCAGCCAGCCGGAGCTACCTGCTCAGCGTGCGGCCCGAGACCAGCTTATCAAGCAACCGGTTGTC TCACCCCAGCTCTGGAAGGAGCACCTTCTGCTCCATCATTGCTCAGCTCACAGAGGAGACCCAGCCGCTA TTTGAGACCACGCTCAAGTCCCGGTCTGTGTCCGAGGACAGCGACGTCAGGTTCACCTGCATCGTCACAG GATACCCAGAGCCAGAGGTGACCTGGTACAAGGATGATACGGAGCTGGACCGCTACTGTGGCTTGCCAAA ATATGAGATCACTCATCAGGGCAACCGCCACACACTGCAGCTGTACAGGTGTCGAGAAGAAGATGCCGCC ATCTACCAGGCCTCTGCCCAGAACAGCAAGGGCATTGTGTCCTGCTCAGGGGTCCTGGAGGTGGGCACCA TGACTGAGTACAAGATCCACCAGCGCTGGTTCGCCAAGTTGAAGCGCAAGGCTGCGGCAAAGCTGCGCGA GATCGAGCAGAGCTGGAAGCACGAGAAGGCGGTGCCTGGGGAGGTCGACACTCTGCGCAAGCTCAGCCCC GACCGCTTCCAGCGAAAGCGGCGATTGAGCGGGGCTCAAGCGCCGGGCCCCTCGGTCCCTACCAGGGAGC CTGAGGGTGGGACCCTGGCGGCTTGGCAGGAGGGAGAGACTGAGACTGCTCAGCACTCAGGTTTGGGCCT GATCAACAGTTTTGCTTCTGGAGAAGTGACCACCAACGGGGAGGCTGCCCCCGAGAATGGAGAGGACGGA GAGCATGGCTTGCTGACATACATCTGTGACGCCATGGAGCTGGGGCCTCAGAGAGCCCTCAAAGAGGAGA GTGGGGCCAAGAAGAAAAAGAAAGATGAGGAATCCAAGCAAGGCCTGCGGAAGCCAGAGTTAGAGAAGGC AGCCCAAAGCCGCCGTTCTTCAGAAAACTGCATCCCCAGCTCAGACGAGCCTGACTCCTGTGGGACTCAG GGGCCCGTGGGCGTGGAGCAGGTTCAGACCCAGCCCAGAGGCAGGGCTGCACGGGGGCCTGGGTCCTCTG GCACAGATAGTACCAGGAAGCCAGCCTCTGCTGTGGGCACTCCAGACAAGGCCCAGAAGGCCCCTGGCCC AGGCCCAGGCCAGGAAGTGTATTTCTCCTTGAAGGACATGTACCTGGAGAACACCCAGGCAGTCAGGCCT CTTGGGGAAGAGGGACCCCAGACCCTGAGTGTCCGGGCGCCTGGGGAGAGTCCCAAGGGGAAGGCACCCC TCAGGGCTAGAAGCGAGGGGGTGCCTGGCGCTCCTGGCCAGCCCACACACTCCTTGACCCCCCAGCCGAC TAGGCCTTTCAACAGAAAGAGATTTGCCCCTCCAAAGCCCAAAGGAGAGGCCACCACTGACAGCAAGCCC ATTTCTTCTCTGAGTCAAGCTCCAGAATGCGGGGCCCAGAGCTTAGGAAAGGCCCCACCTCAGGCCTCTG TGCAGGTGCCGACGCCCCCTGCCCGGCGGAGACATGGCACCCGGGACAGCACGTTGCAGGGGCAAGCAGG CCACAGGACTCCAGGAGAGGTCCTGGAATGCCAGACAACCACGGCTCCTACCATGTCGGCCAGCAGCAGC TCTGATGTAGCCTCCATTGGGGTTAGCACTTCCGGAAGTCAAGGTATCATTGAACCCATGGATATGGAAA CCCAGGAGGATGGGAGAACATCTGCTAACCAGAGAACTGGAAGCAAGAAGAATGTGCAGGCAGATGGGAA GATACAAGTGGATGGAAGGACCAGGGGAGATGGAACACAGACAGCCCAGAGGACACGTGCAGATAGGAAG ACGCAGGTGGATGCTGGGACACAAGAAAGCAAGAGGCCACAGTCAGACAGGAGTGCACAGAAGGGCATGA TGACACAGGGAAGGGCAGAGACACAGCTAGAAACAACACAGGCAGGTGAGAAGATACAGGAAGACAGGAA GGCCCAGGCAGATAAGGGCACACAGGAAGACAGAAGGATGCAGGGAGAGAAGGGGATGCAGGGAGAGAAG GGGACGCAGTCAGAGGGGAGCGCGCCCACAGCCATGGAAGGTCAGTCTGAGCAAGAGGTGGCAACCAGCC TCGGCCCACCATCCAGAACCCCCAAACTCCCACCTACAGCGGGTCCTAGAGCTCCTCTGAATATTGAATG TTTTGTACAGACCCCAGAAGGGTCTTGTTTCCCAAAAAAACCTGGTTGCCTGCCCAGATCTGAGGAGGCA GTAGTAACAGCCTCCAGGAACCATGAGCAAACTGTGCTGGGTCCCCTGTCAGGGAACCTCATGCTCCCAG CACAGCCGCCCCATGAGGGGAGTGTGGAGCAGGTGGGAGGAGAGAGATGCCGAGGGCCACAGTCATCAGG CCCAGTCGAGGCCAAGCAGGAGGACAGCCCGTTCCAGTGCCCCAAGGAGGAGCGGCCAGGGGGAGTGCCG TGTATGGATCAGGGTGGCTGTCCTCTAGCTGGCCTGAGCCAGGAGGTACCCACGATGCCTTCTCTTCCTG GAACTGGGCTGACAGCTAGCCCAAAGGCGGGGCCGTGTAGCACCCCGACTTCTCAGCACGGGAGCACAGC CACCTTCCTGCCCTCTGAGGATCAGGTCCTGATGAGTTCTGCCCCAACACTGCACCTGGGGCTGGGGACC CCCACTCAGAGTCACCCACCAGAAACCATGGCCACCAGCAGTGAGGGGGCCTGCGCCCAGGTACCAGATG TGGAGGGGCGGACCCCAGGTCCCCGGAGCTGTGACCCTGGCCTCATAGATTCCCTGAAGAACTACCTGCT TCTGCTGCTGAAGCTGTCCAGCACAGAGACAAGTGGAGCAGGGGGAGAGTCCCAGGTGGGGGCAGCCACC GGAGGTCTGGTGCCCTCAGCCACTCTGACACCCACTGTGGAAGTGGCTGGGCTTAGTCCCCGGACATCGA GGCGCATCCTGGAGCGTGTGGAGAACAACCACCTGGTGCAGAGTGCACAGACCCTGCTGCTGAGCCCCTG TACCTCCCGCCGCCTCACCGGCCTCCTGGACCGTGAGGTGCAGGCTGGCCGCCAGGCCCTTGCTGCTGCC CGAGGCTCCTGGGGTCCTGGTCCCAGCTCCCTCACTGTCCCTGCCATTGTGGTAGACGAGGAGGACCCTG GGCTGGCCTCAGAAGGAGCCAGTGAGGGTGAAGGAGAGGTTTCCCCTGAGGGGCCTGGCCTCCTGGGGGC CTCTCAGGAGAGCAGCATGGCTGGTCGACTGGGGGAGGCGGGTGGGCAGGCAGCCCCTGGACAGGGGCCC TCAGCAGAGAGCATAGCCCAGGAGCCCTCCCAAGAGGAGAAGTTCCCAGGGGAGGCTCTGACAGGTCTCC CGGCAGCTACACCTGAGGAACTGGCTCTAGGGGCCCGGAGGAAGAGATTTCTCCCTAAGGTCAGAGCAGC AGGAGACGGGGAGGCAACCACACCTGAAGAAAGGGAGAGCCCCACGGTTTCCCCCCGGGGGCCCAGGAAA AGCCTGGTGCCTGGGTCCCCAGGGACTCCAGGGCGGGAGAGACGCTCCCCTACGCAGGGCAGAAAGGCGA GCATGCTGGAGGTGCCTCGGGCAGAGGAGGAGCTGGCGGCAGGAGACCTGGGCCCCAGCCCCAAGGCCGG CGGTCTGGACACAGAGGTGGCCCTGGATGAAGGCAAGCAGGAGACACTGGCCAAGCCCAGGAAAGCCAAA GACCTGCTGAAAGCCCCACAGGTGATCCGGAAGATTCGGGTGGAGCAGTTTCCTGATGCCTCCGGTAGCC TGAAGCTGTGGTGCCAGTTTTTCAACATTCTTAGTGACTCAGTCTTGACATGGGCCAAGGATCAGCGCCC AGTGGGCGAGGTGGGCAGGAGCGCAGGGGATGAGGGGCCGGCGGCCTTGGCCATCGTGCAGGCCTCCCCC GTAGACTGCGGTGTGTATCGGTGCACCATCCACAATGAGCACGGCTCGGCCTCCACCGACTTCTGCCTCA GCCCTGAGGTGTTGTCAGGATTCATCTCCAGAGAAGAAGGTGAAGTTGGAGAAGAGATTGAGATGACCCC TATGGTGTTTGCTAAGGGTCTGGCTGACTCTGGCTGCTGGGGGGACAAGCTCTTTGGGCGACTGGTAAGC GAGGAGCTCCGAGGGGGTGGATATGGGTGTGGCCTTCGGAAGGCCTCCCAGGCCAAGGTCATCTACGGGC TGGAACCCATCTTCGAGTCGGGCCGCACGTGCATCATCAAGGTGTCCAGCCTGCTTGTGTTTGGGCCCAG CAGTGAGACTTCTCTTGTGGGCAGAAACTACGACGTCACCATCCAGGGGTGCAAGATCCAGAACATGAGT CGGGAGTACTGCAAAATCTTCGCAGCAGAAGCCCGGGCCGCGCCTGGCTTTGGGGAGGTGCCTGAGATCA TCCCACTGTATCTGATCTACCGGCCTGCAAACAATATCCCATATGCTACCCTGGAGGAAGACCTGGGCAA GCCCCTGGAGTCTTACTGTTCTCGGGAATGGGGCTGTGCTGAGGCTCCGACAGCATCTGGCAGCTCTGAG GCCATGCAGAAATGCCAGACCTTCCAACACTGGCTGTATCAGTGGACAAATGGCAGCTTCCTTGTCACAG ACTTGGCAGGGGTTGACTGGAAGATGACTGATGTGCAGATTGCTACCAAACTCCGAGGATACCAGGGCCT CAAGGAAAGCTGCTTCCCTGCCCTGCTGGACCGGTTCGCCTCCTCCCACCAGTGCAATGCCTACTGTGAG CTGCTGGGGCTGACACCTCTCAAGGGCCCGGAGGCGGCCCACCCCCAAGCCAAAGCCAAAGGCTCTAAGA GTCCATCTGCTGGCAGGAAAGGCTCCCAGCTGAGTCCTCAGCCCCAGAAGAAAGGCCTCCCTAGTCCTCA GGGCACCCGGAAGAGTGCTCCAAGTTCCAAGGCCACCCCTCAGGCCTCAGAGCCAGTCACCACTCAGTTG TTGGGACAGCCTCCCACCCAAGAGGAGGGCTCCAAGGCCCAGGGCATGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220076 representing NM_020778
Red=Cloning site Green=Tags(s) MEVAWLVYVLGQQPLARQGEGQSRLVPGRGLVLWLPGLPRSSPSWPAVDLAPLAPARPRGPLICHTGHEQ AGREPGPGSSTKGPVLHDQDTRCAFLPRPPGPLQTRRYCRHQGRQGSGLGAGPGAGTWAPAPPGVSKPRC PGRARPGEGQQQVTTARPPAINRGARQPRAGAAAAGRGPGAGAWRTGEAAASAGPAVGEGGAMGSRRAPT RGWGAGGRSGAGGDGEDDGPVWIPSPASRSYLLSVRPETSLSSNRLSHPSSGRSTFCSIIAQLTEETQPL FETTLKSRSVSEDSDVRFTCIVTGYPEPEVTWYKDDTELDRYCGLPKYEITHQGNRHTLQLYRCREEDAA IYQASAQNSKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKLREIEQSWKHEKAVPGEVDTLRKLSP DRFQRKRRLSGAQAPGPSVPTREPEGGTLAAWQEGETETAQHSGLGLINSFASGEVTTNGEAAPENGEDG EHGLLTYICDAMELGPQRALKEESGAKKKKKDEESKQGLRKPELEKAAQSRRSSENCIPSSDEPDSCGTQ GPVGVEQVQTQPRGRAARGPGSSGTDSTRKPASAVGTPDKAQKAPGPGPGQEVYFSLKDMYLENTQAVRP LGEEGPQTLSVRAPGESPKGKAPLRARSEGVPGAPGQPTHSLTPQPTRPFNRKRFAPPKPKGEATTDSKP ISSLSQAPECGAQSLGKAPPQASVQVPTPPARRRHGTRDSTLQGQAGHRTPGEVLECQTTTAPTMSASSS SDVASIGVSTSGSQGIIEPMDMETQEDGRTSANQRTGSKKNVQADGKIQVDGRTRGDGTQTAQRTRADRK TQVDAGTQESKRPQSDRSAQKGMMTQGRAETQLETTQAGEKIQEDRKAQADKGTQEDRRMQGEKGMQGEK GTQSEGSAPTAMEGQSEQEVATSLGPPSRTPKLPPTAGPRAPLNIECFVQTPEGSCFPKKPGCLPRSEEA VVTASRNHEQTVLGPLSGNLMLPAQPPHEGSVEQVGGERCRGPQSSGPVEAKQEDSPFQCPKEERPGGVP CMDQGGCPLAGLSQEVPTMPSLPGTGLTASPKAGPCSTPTSQHGSTATFLPSEDQVLMSSAPTLHLGLGT PTQSHPPETMATSSEGACAQVPDVEGRTPGPRSCDPGLIDSLKNYLLLLLKLSSTETSGAGGESQVGAAT GGLVPSATLTPTVEVAGLSPRTSRRILERVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAGRQALAAA RGSWGPGPSSLTVPAIVVDEEDPGLASEGASEGEGEVSPEGPGLLGASQESSMAGRLGEAGGQAAPGQGP SAESIAQEPSQEEKFPGEALTGLPAATPEELALGARRKRFLPKVRAAGDGEATTPEERESPTVSPRGPRK SLVPGSPGTPGRERRSPTQGRKASMLEVPRAEEELAAGDLGPSPKAGGLDTEVALDEGKQETLAKPRKAK DLLKAPQVIRKIRVEQFPDASGSLKLWCQFFNILSDSVLTWAKDQRPVGEVGRSAGDEGPAALAIVQASP VDCGVYRCTIHNEHGSASTDFCLSPEVLSGFISREEGEVGEEIEMTPMVFAKGLADSGCWGDKLFGRLVS EELRGGGYGCGLRKASQAKVIYGLEPIFESGRTCIIKVSSLLVFGPSSETSLVGRNYDVTIQGCKIQNMS REYCKIFAAEARAAPGFGEVPEIIPLYLIYRPANNIPYATLEEDLGKPLESYCSREWGCAEAPTASGSSE AMQKCQTFQHWLYQWTNGSFLVTDLAGVDWKMTDVQIATKLRGYQGLKESCFPALLDRFASSHQCNAYCE LLGLTPLKGPEAAHPQAKAKGSKSPSAGRKGSQLSPQPQKKGLPSPQGTRKSAPSSKATPQASEPVTTQL LGQPPTQEEGSKAQGMR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020778 |
| ORF Size | 5721 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020778.2, NP_065829.2 |
| RefSeq Size | 10914 bp |
| RefSeq ORF | 5118 bp |
| Locus ID | 57538 |
| UniProt ID | Q96L96 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 201.1 kDa |
| Gene Summary | Involved in cardiomyocyte differentiation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pathogenesis of Cardiomyopathy Caused by Variants in ALPK3 , an Essential Pseudokinase in the Cardiomyocyte Nucleus and Sarcomere
,null,
Circulation
,PubMed ID 36321451
[ALPK3]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220076L1 | Lenti ORF clone of Human alpha-kinase 3 (ALPK3), Myc-DDK-tagged |
CNY 21,624.00 |
|
| RG220076 | ALPK3 (tGFP-tagged) - Human alpha-kinase 3 (ALPK3) |
CNY 20,900.00 |
|
| SC309405 | ALPK3 (untagged)-Human alpha-kinase 3 (ALPK3) |
CNY 36,670.00 |
