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IL16 (NM_004513) Human Tagged ORF Clone
CAT＃: RC220031
IL16 (Myc-DDK-tagged)-Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 1
ORF Plasmid: tGFP
"NM_004513" in other vectors (4)
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CNY 4,696.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LCF; NIL16; prIL-16; PRIL16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220031 representing NM_004513
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTATAGCTTTGATACCACAGCCGAAGACCCTTGGGTTAGGATTTCTGACTGCATCAAAAACTTAT TTAGCCCCATCATGAGTGAGAACCATGGCCACATGCCTCTACAGCCCAATGCCAGCCTGAATGAAGAAGA AGGGACACAGGGCCACCCAGATGGGACCCCACCAAAGCTGGACACCGCCAATGGCACTCCCAAAGTTTAC AAGTCAGCAGACAGCAGCACTGTGAAGAAAGGTCCTCCTGTGGCTCCCAAGCCAGCCTGGTTTCGCCAAA GCTTGAAAGGTTTGAGGAATCGTGCTTCAGACCCAAGAGGGCTCCCTGATCCTGCCTTGTCCACCCAGCC AGCACCTGCTTCCAGGGAGCACCTAGGATCACACATCCGGGCCTCCTCCTCCTCCTCCTCCATCAGGCAG AGAATCAGCTCCTTTGAAACCTTTGGCTCCTCTCAACTGCCTGACAAAGGAGCCCAGAGACTGAGCCTCC AGCCCTCCTCTGGGGAGGCAGCAAAACCTCTTGGGAAGCATGAGGAAGGACGGTTTTCTGGACTCTTGGG GCGAGGGGCTGCACCCACTCTTGTGCCCCAGCAGCCTGAGCAAGTACTGTCCTCGGGGTCCCCTGCAGCC TCCGAGGCCAGAGACCCAGGTGTGTCTGAGTCCCCTCCCCCAGGGCGGCAGCCCAATCAGAAAACTCTCC CCCCTGGCCCGGACCCGCTCCTAAGGCTGCTGTCAACACAGGCTGAGGAATCTCAAGGCCCAGTGCTCAA GATGCCTAGCCAGCGAGCACGGAGCTTCCCCCTGACCAGGTCCCAGTCCTGTGAGACGAAGCTACTTGAC GAAAAGACCAGCAAACTCTATTCTATCAGCAGCCAAGTGTCATCGGCTGTCATGAAATCCTTGCTGTGCC TTCCATCTTCTATCTCCTGTGCCCAGACTCCCTGCATCCCCAAGGAAGGGGCATCTCCAACATCATCATC CAACGAAGACTCAGCTGCAAATGGTTCTGCTGAAACATCTGCCTTGGACACAGGGTTCTCGCTCAACCTT TCAGAGCTGAGAGAATATACAGAGGGTCTCACGGAAGCCAAGGAAGACGATGATGGGGACCACAGTTCCC TTCAGTCTGGTCAGTCCGTTATCTCCCTGCTGAGCTCAGAAGAATTAAAAAAACTCATCGAGGAGGTGAA GGTTCTGGATGAAGCAACATTAAAGCAATTAGACGGCATCCATGTCACCATCTTACACAAGGAGGAAGGT GCTGGTCTTGGGTTCAGCTTGGCAGGAGGAGCAGATCTAGAAAACAAGGTGATTACGGTTCACAGAGTGT TTCCAAATGGGCTGGCCTCCCAGGAAGGGACTATTCAGAAGGGCAATGAGGTTCTTTCCATCAACGGCAA GTCTCTCAAGGGGACCACGCACCATGATGCCTTGGCCATCCTCCGCCAAGCTCGAGAGCCCAGGCAAGCT GTGATTGTCACAAGGAAGCTGACTCCAGAGGCCATGCCCGACCTCAACTCCTCCACTGACTCTGCAGCCT CAGCCTCTGCAGCCAGTGATGTTTCTGTAGAATCTACAGCAGAGGCCACAGTCTGCACGGTGACACTGGA GAAGATGTCGGCAGGGCTGGGCTTCAGCCTGGAAGGAGGGAAGGGCTCCCTACACGGAGACAAGCCTCTC ACCATTAACAGGATTTTCAAAGGAGCAGCCTCAGAACAAAGTGAGACAGTCCAGCCTGGAGATGAAATCT TGCAGCTGGGTGGCACTGCCATGCAGGGCCTCACACGGTTTGAAGCCTGGAACATCATCAAGGCACTGCC TGATGGACCTGTCACGATTGTCATCAGGAGAAAAAGCCTCCAGTCCAAGGAAACCACAGCTGCTGGAGAC TCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220031 representing NM_004513
Red=Cloning site Green=Tags(s) MDYSFDTTAEDPWVRISDCIKNLFSPIMSENHGHMPLQPNASLNEEEGTQGHPDGTPPKLDTANGTPKVY KSADSSTVKKGPPVAPKPAWFRQSLKGLRNRASDPRGLPDPALSTQPAPASREHLGSHIRASSSSSSIRQ RISSFETFGSSQLPDKGAQRLSLQPSSGEAAKPLGKHEEGRFSGLLGRGAAPTLVPQQPEQVLSSGSPAA SEARDPGVSESPPPGRQPNQKTLPPGPDPLLRLLSTQAEESQGPVLKMPSQRARSFPLTRSQSCETKLLD EKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNL SELREYTEGLTEAKEDDDGDHSSLQSGQSVISLLSSEELKKLIEEVKVLDEATLKQLDGIHVTILHKEEG AGLGFSLAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGTTHHDALAILRQAREPRQA VIVTRKLTPEAMPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPL TINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGD S TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004513 |
| ORF Size | 1893 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004513.5 |
| RefSeq Size | 6193 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 3603 |
| UniProt ID | Q14005 |
| Domains | PDZ |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 66.5 kDa |
| Gene Summary | The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220031L3 | Lenti ORF clone of Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC220031L4 | Lenti ORF clone of Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG220031 | IL16 (tGFP-tagged) - Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 1 |
CNY 6,296.00 |
|
| SC109345 | IL16 (untagged)-Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 1 |
CNY 4,704.00 |
