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NIRF (UHRF2) (NM_152896) Human Tagged ORF Clone
CAT＃: RC219579
UHRF2 (Myc-DDK-tagged)-Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2)
ORF Plasmid: tGFP
"NM_152896" in other vectors (6)
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CNY 5,872.00
CNY 8,460.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NIRF; RNF107; TDRD23; URF2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219579 representing NM_152896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATACAGGTTCGCACCATTGATGGCTCCAAGACGTGCACCATTGAGGACGTGTCTCGCAAAGCCA CGATTGAGGAGCTGCGCGAGCGGGTGTGGGCGCTGTTCGACGTGCGGCCCGAATGCCAGCGCCTCTTCTA CCGGGGCAAGCAGTTGGAAAATGGATATACCTTATTTGATTATGATGTTGGACTGAATGATATAATTCAG CTGCTAGTTCGCCCAGACCCTGATCATCTTCCTGGCACATCTACACAGATTGAGGCTAAACCCTGTTCTA ATAGTCCACCTAAAGTAAAGAAAGCTCCGAGGGTAGGACCTTCCAATCAGCCATCTACATCAGCTCGTGC CCGTCTTATTGATCCTGGCTTTGGAATATATAAGGTAAATGAATTGGTGGATGCCAGAGATGTCGGCCTT GGTGCTTGGTTTGAAGCACACATACATAGTGTTACTAGAGCTTCTGATGGACAGTCACGTGGCAAAACTC CACTGAAGAATGGCAGTTCTTGTAAAAGGACTAATGGAAATATAAAGCATAAATCCAAAGAGAACACAAA TAAATTGGACAGTGTACCCTCTACGTCTAATTCAGACTGTGTTGCTGCTGATGAAGACGTTATTTACCAT ATCCAGTATGATGAATACCCAGAAAGCGGTACTCTAGAAATGAATGTCAAGGATCTTAGACCACGAGCTA GAACCATTTTGAAATGGAATGAACTAAATGTTGGTGATGTGGTAATGGTTAATTATAATGTAGAAAGTCC TGGACAAAGAGGATTCTGGTTTGATGCAGAAATTACCACATTGAAGACAATCTCAAGGACCAAAAAAGAA CTTCGTGTGAAAATTTTCCTGGGGGGTTCTGAAGGAACATTAAATGACTGCAAGATAATATCTGTAGATG AAATCTTCAAGATTGAGAGACCTGGAGCCCATCCCCTTTCATTTGCAGATGGAAAGTTTTTAAGGCGAAA TGACCCTGAATGTGACCTGTGTGGTGGAGACCCAGAAAAGAAATGTCATTCTTGCTCCTGTCGTGTATGT GGTGGGAAACATGAACCCAACATGCAGCTTCTGTGTGATGAATGTAATGTGGCTTATCATATTTACTGTC TGAATCCACCTTTGGATAAAGTCCCAGAAGAGGAATACTGGTATTGTCCTTCTTGTAAAACTGATTCCAG TGAAGTTGTAAAGGCTGGTGAAAGACTCAAGATGAGTAAAAAGAAAGCAAAGATGCCGTCAGCTAGTACT GAAAGCCGAAGAGACTGGGGCAGGGGAATGGCTTGTGTTGGTCGTACGAGAGAATGTACTATTGTCCCTT CTAATCATTATGGACCCATTCCTGGTATTCCTGTTGGATCAACTTGGAGATTTAGAGTTCAGGTGAGCGA AGCAGGTGTTCACAGACCCCATGTTGGTGGAATTCATGGTCGAAGTAATGATGGGGCTTATTCTCTTGTA CTGGCTGGTGGATTTGCGGATGAAGTCGACCGAGGTGATGAGTTCACATACACTGGAAGCGGTGGTAAAA ATCTTGCTGGTAACAAAAGAATTGGTGCACCTTCAGCTGATCAAACATTAACAAACATGAACAGGGCATT GGCCCTAAACTGTGATGCTCCATTGGATGATAAAATTGGAGCAGAGTCTCGGAATTGGAGAGCTGGTAAG CCAGTCAGAGTGATACGCAGTTTTAAAGGGAGGAAGATCAGCAAATATGCTCCTGAAGAAGGCAACAGAT ATGATGGCATTTATAAGGTGGTGAAATACTGGCCAGAGATTTCATCAAGCCATGGATTCTTGGTTTGGCG CTATCTTTTAAGAAGAGATGATGTTGAACCTGCTCCTTGGACCTCTGAAGGAATAGAACGGTCAAGGAGA TTATGTCTACGTTTACAGTATCCAGCAGGTTACCCTTCAGATAAAGAAGGGAAGAAGCCTAAAGGACAGT CAAAGAAGCAGCCCAGTGGAACCACAAAAAGGCCAATTTCAGATGATGACTGTCCAAGTGCCTCCAAAGT GTACAAAGCATCAGATTCAGCAGAAGCAATTGAGGCTTTTCAACTAACTCCTCAACAGCAACATCTCATC AGAGAAGATTGTCAAAACCAGAAGCTGTGGGATGAAGTGCTTTCACATCTTGTGGAAGGACCAAATTTTC TGAAAAAATTGGAACAATCTTTTATGTGCGTTTGCTGTCAGGAGCTAGTTTACCAGCCTGTGACAACTGA GTGCTTCCACAATGTCTGTAAAGATTGCCTACAGCGCTCCTTTAAGGCACAGGTTTTCTCCTGCCCTGCT TGCCGGCATGATCTTGGCCAGAATTACATCATGATTCCCAATGAGATTCTGCAGACTCTACTTGACCTTT TCTTCCCTGGCTACAGCAAAGGACGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219579 representing NM_152896
Red=Cloning site Green=Tags(s) MWIQVRTIDGSKTCTIEDVSRKATIEELRERVWALFDVRPECQRLFYRGKQLENGYTLFDYDVGLNDIIQ LLVRPDPDHLPGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARARLIDPGFGIYKVNELVDARDVGL GAWFEAHIHSVTRASDGQSRGKTPLKNGSSCKRTNGNIKHKSKENTNKLDSVPSTSNSDCVAADEDVIYH IQYDEYPESGTLEMNVKDLRPRARTILKWNELNVGDVVMVNYNVESPGQRGFWFDAEITTLKTISRTKKE LRVKIFLGGSEGTLNDCKIISVDEIFKIERPGAHPLSFADGKFLRRNDPECDLCGGDPEKKCHSCSCRVC GGKHEPNMQLLCDECNVAYHIYCLNPPLDKVPEEEYWYCPSCKTDSSEVVKAGERLKMSKKKAKMPSAST ESRRDWGRGMACVGRTRECTIVPSNHYGPIPGIPVGSTWRFRVQVSEAGVHRPHVGGIHGRSNDGAYSLV LAGGFADEVDRGDEFTYTGSGGKNLAGNKRIGAPSADQTLTNMNRALALNCDAPLDDKIGAESRNWRAGK PVRVIRSFKGRKISKYAPEEGNRYDGIYKVVKYWPEISSSHGFLVWRYLLRRDDVEPAPWTSEGIERSRR LCLRLQYPAGYPSDKEGKKPKGQSKKQPSGTTKRPISDDDCPSASKVYKASDSAEAIEAFQLTPQQQHLI REDCQNQKLWDEVLSHLVEGPNFLKKLEQSFMCVCCQELVYQPVTTECFHNVCKDCLQRSFKAQVFSCPA CRHDLGQNYIMIPNEILQTLLDLFFPGYSKGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152896 |
| ORF Size | 2406 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152896.3 |
| RefSeq Size | 3621 bp |
| RefSeq ORF | 2409 bp |
| Locus ID | 115426 |
| UniProt ID | Q96PU4 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 89.8 kDa |
| Gene Summary | This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219579L1 | Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), Myc-DDK-tagged |
CNY 8,272.00 |
|
| RC219579L2 | Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), mGFP tagged |
CNY 10,360.00 |
|
| RC219579L3 | Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), Myc-DDK-tagged |
CNY 10,360.00 |
|
| RC219579L4 | Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), mGFP tagged |
CNY 10,360.00 |
|
| RG219579 | UHRF2 (tGFP-tagged) - Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2) |
CNY 7,472.00 |
|
| SC124707 | UHRF2 (untagged)-Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2) |
CNY 5,880.00 |
