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CD101 (NM_004258) Human Tagged ORF Clone
CAT＃: RC219415
CD101 (Myc-DDK-tagged)-Human CD101 molecule (CD101)
ORF Plasmid: tGFP
"NM_004258" in other vectors (6)
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CNY 10,664.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EWI-101; IGSF2; V7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219415 representing NM_004258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCATCTCATATGTGGCATCTTTCTTTCTCCTTCTGACTAAGCTCAGCATTGGCCAGAGAGAAG TAACAGTTCAGAAAGGACCACTGTTTAGAGCTGAAGGTTACCCAGTCAGCATTGGCTGCAATGTAACTGG CCACCAGGGACCTTCTGAGCAGCATTTCCAGTGGTCTGTTTACCTGCCGACAAACCCGACCCAGGAAGTC CAGATCATTAGCACCAAGGATGCTGCCTTCTCTTACGCAGTATATACGCAGCGGGTGCGAAGCGGAGACG TCTACGTGGAGAGGGTCCAGGGCAACTCAGTCTTGTTGCACATCTCAAAACTCCAGATGAAGGATGCTGG CGAGTATGAGTGTCACACACCAAACACTGATGAGAAATACTATGGAAGTTACAGTGCAAAGACTAATCTA ATTGTTATTCCAGATACCCTCTCTGCCACCATGAGTTCTCAGACTCTCGGTAAGGAGGAAGGTGAGCCAT TAGCCCTCACCTGTGAGGCATCCAAAGCCACAGCCCAACATACTCACCTCTCTGTCACCTGGTACCTAAC ACAGGATGGAGGAGGAAGCCAAGCCACTGAGATTATTTCTCTCTCCAAAGATTTTATATTGGTCCCTGGG CCCTTGTATACAGAGCGGTTTGCAGCCAGTGACGTACAGCTCAACAAACTGGGACCCACTACATTCAGGC TGTCCATAGAGAGGCTCCAGTCCTCAGATCAGGGTCAGCTGTTCTGTGAGGCAACGGAATGGATTCAGGA TCCAGATGAAACTTGGATGTTCATCACCAAAAAGCAGACCGATCAAACCACTCTGAGGATCCAGCCAGCA GTGAAAGATTTTCAAGTCAACATTACAGCTGACAGCTTGTTTGCTGAAGGGAAACCCTTAGAACTGGTTT GCCTGGTTGTAAGCAGTGGCCGTGACCCACAGCTTCAAGGCATTTGGTTCTTCAATGGGACTGAAATTGC TCACATTGATGCTGGTGGAGTCCTGGGCCTGAAGAATGACTACAAAGAGAGAGCAAGTCAAGGAGAGCTC CAGGTTTCAAAGTTAGGCCCCAAGGCTTTCTCTCTCAAGATCTTCTCTCTGGGCCCAGAGGATGAAGGCG CCTACAGATGTGTGGTAGCAGAGGTCATGAAAACACGCACAGGTTCCTGGCAGGTGCTTCAGAGAAAGCA GTCACCAGACAGCCACGTGCACCTGAGGAAGCCAGCAGCAAGAAGTGTGGTCATGTCTACCAAGAACAAG CAGCAAGTTGTGTGGGAAGGAGAGACACTCGCCTTTCTCTGTAAGGCTGGTGGAGCTGAAAGTCCCCTGT CTGTGAGCTGGTGGCACATCCCACGGGACCAGACACAGCCCGAGTTTGTGGCTGGCATGGGGCAGGATGG CATTGTGCAGCTGGGTGCCTCCTATGGGGTACCCAGTTACCATGGCAACACAAGGCTGGAGAAAATGGAC TGGGCCACCTTCCAGCTGGAGATCACCTTCACTGCCATCACAGACAGTGGCACATATGAGTGCAGAGTAT CTGAGAAGTCTCGGAACCAGGCCAGAGATCTGAGCTGGACTCAGAAGATTTCAGTTACTGTAAAGTCTCT GGAGTCAAGTTTACAAGTTAGTCTGATGAGCCGTCAGCCGCAAGTGATGTTAACCAACACCTTTGACCTG TCCTGTGTCGTGAGGGCCGGTTACTCTGACCTCAAGGTGCCACTCACTGTGACGTGGCAGTTCCAGCCAG CTAGCTCTCACATCTTCCACCAGCTTATTCGAATCACCCACAATGGCACTATTGAATGGGGGAATTTCCT ATCCCGGTTCCAAAAGAAGACGAAAGTGTCGCAGTCTTTATTTCGTTCACAACTCCTAGTCCATGATGCC ACTGAGGAAGAGACAGGAGTGTATCAGTGTGAAGTAGAAGTTTATGACAGAAATTCCCTATACAACAACC GCCCCCCGAGGGCTTCTGCCATCTCTCACCCACTGAGGATAGCCGTCACTTTACCAGAGAGCAAGCTAAA AGTGAATTCAAGGAGTCAAGTCCAAGAGCTCTCCATCAACTCCAACACTGATATAGAATGTAGCATCTTG TCCCGGTCCAATGGAAACCTTCAGTTAGCCATTATTTGGTATTTTTCTCCTGTTTCCACTAATGCCTCTT GGCTAAAGATCCTGGAGATGGACCAAACCAATGTTATAAAAACTGGGGATGAGTTTCACACCCCACAGAG AAAACAAAAATTTCATACTGAGAAGGTTTCCCAAGACTTATTTCAGCTGCACATTCTGAATGTGGAAGAC AGCGATCGGGGCAAATATCACTGTGCTGTGGAGGAATGGCTCCTGTCTACAAATGGCACTTGGCACAAGC TTGGAGAAAAGAAGTCAGGACTAACAGAATTGAAACTCAAGCCCACAGGAAGTAAGGTACGTGTCTCCAA AGTGTACTGGACCGAAAATGTGACTGAGCACAGAGAAGTGGCCATCCGCTGCAGCCTGGAGAGTGTAGGC AGCTCAGCCACTCTGTACTCTGTGATGTGGTACTGGAACAGAGAAAACTCTGGAAGTAAATTGCTGGTGC ACTTGCAACATGATGGCTTGCTGGAGTATGGGGAAGAGGGGCTCAGGAGGCACCTGCACTGTTACCGTTC ATCCTCTACAGACTTTGTCCTGAAGCTTCATCAGGTGGAGATGGAGGATGCAGGAATGTACTGGTGTAGG GTGGCAGAGTGGCAGCTCCATGGACACCCAAGCAAGTGGATTAATCAAGCATCCGATGAGTCACAGCGGA TGGTGCTCACGGTGCTGCCTTCAGAGCCCACGCTTCCTTCCAGGATCTGCTCCTCGGCCCCTTTACTCTA TTTCCTGTTCATCTGTCCCTTCGTCCTGCTCCTCCTTCTGCTCATCTCCCTCCTCTGCTTATACTGGAAG GCCAGGAAGTTGTCAACACTGCGTTCCAACACACGGAAAGAAAAAGCTCTCTGGGTGGACTTGAAAGAGG CTGGAGGTGTGACCACAAATAGGAGGGAAGACGAGGAGGAAGATGAAGGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219415 representing NM_004258
Red=Cloning site Green=Tags(s) MAGISYVASFFLLLTKLSIGQREVTVQKGPLFRAEGYPVSIGCNVTGHQGPSEQHFQWSVYLPTNPTQEV QIISTKDAAFSYAVYTQRVRSGDVYVERVQGNSVLLHISKLQMKDAGEYECHTPNTDEKYYGSYSAKTNL IVIPDTLSATMSSQTLGKEEGEPLALTCEASKATAQHTHLSVTWYLTQDGGGSQATEIISLSKDFILVPG PLYTERFAASDVQLNKLGPTTFRLSIERLQSSDQGQLFCEATEWIQDPDETWMFITKKQTDQTTLRIQPA VKDFQVNITADSLFAEGKPLELVCLVVSSGRDPQLQGIWFFNGTEIAHIDAGGVLGLKNDYKERASQGEL QVSKLGPKAFSLKIFSLGPEDEGAYRCVVAEVMKTRTGSWQVLQRKQSPDSHVHLRKPAARSVVMSTKNK QQVVWEGETLAFLCKAGGAESPLSVSWWHIPRDQTQPEFVAGMGQDGIVQLGASYGVPSYHGNTRLEKMD WATFQLEITFTAITDSGTYECRVSEKSRNQARDLSWTQKISVTVKSLESSLQVSLMSRQPQVMLTNTFDL SCVVRAGYSDLKVPLTVTWQFQPASSHIFHQLIRITHNGTIEWGNFLSRFQKKTKVSQSLFRSQLLVHDA TEEETGVYQCEVEVYDRNSLYNNRPPRASAISHPLRIAVTLPESKLKVNSRSQVQELSINSNTDIECSIL SRSNGNLQLAIIWYFSPVSTNASWLKILEMDQTNVIKTGDEFHTPQRKQKFHTEKVSQDLFQLHILNVED SDRGKYHCAVEEWLLSTNGTWHKLGEKKSGLTELKLKPTGSKVRVSKVYWTENVTEHREVAIRCSLESVG SSATLYSVMWYWNRENSGSKLLVHLQHDGLLEYGEEGLRRHLHCYRSSSTDFVLKLHQVEMEDAGMYWCR VAEWQLHGHPSKWINQASDESQRMVLTVLPSEPTLPSRICSSAPLLYFLFICPFVLLLLLLISLLCLYWK ARKLSTLRSNTRKEKALWVDLKEAGGVTTNRREDEEEDEGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004258 |
| ORF Size | 3063 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004258.5 |
| RefSeq Size | 3557 bp |
| RefSeq ORF | 3066 bp |
| Locus ID | 9398 |
| UniProt ID | Q93033 |
| Domains | ig, IGv, IG |
| Protein Families | Transmembrane |
| MW | 115.1 kDa |
| Gene Summary | Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219415L1 | Lenti-ORF clone of CD101 (Myc-DDK-tagged)-Human CD101 molecule (CD101) |
CNY 7,600.00 |
|
| RC219415L2 | Lenti-ORF clone of CD101 (mGFP-tagged)-Human CD101 molecule (CD101) |
CNY 7,600.00 |
|
| RC219415L3 | Lenti-ORF clone of CD101 (Myc-DDK-tagged)-Human CD101 molecule (CD101) |
CNY 7,600.00 |
|
| RC219415L4 | Lenti-ORF clone of CD101 (mGFP-tagged)-Human CD101 molecule (CD101) |
CNY 7,600.00 |
|
| RG219415 | CD101 (tGFP-tagged) - Human CD101 molecule (CD101) |
CNY 12,264.00 |
|
| SC117463 | CD101 (untagged)-Human CD101 molecule (CD101) |
CNY 10,672.00 |
