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MAP3K19 (NM_001018046) Human Tagged ORF Clone
CAT＃: RC219370
MAP3K19 (Myc-DDK-tagged)-Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2
ORF Plasmid: tGFP
"NM_001018046" in other vectors (6)
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CNY 3,800.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RCK; YSK4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219370 representing NM_001018046
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTATGCCAAAACCAGAAAGACATGCTGAGTCATTGCTTGACATTTGTCATGATACAAACTCTT CTCCAACTGATTTGATGACAGTTACCAAAAATCAAAACATCATCTTGCAAAGCATCAGCAGAAGTGAGGA GTTCGACCAAGATGGTGACTGCAGTCATTCCACACTGGTTAATGAAGAAGAAGATCCCAGTGGTGGTAGA CAGGACTGGCAACCCAGGACAGAAGGTGTTGAGATCACTGTAACTTTTCCAAGAGATGTCAGTCCTCCCC AAGAAATGAGCCAAGAAGACTTAAAAGAAAAGAATCTGATAAACTCATCGCTTCAAGAATGGGCACAAGC ACATGCAGTTTCTCATCCAAATGAAATAGAAACGGTGGAGCTCAGGAAAAAGAAGCTGACCATGCGGCCC TTAGTTTTGCAAAAAGAGGAAAGTTCCAGGGAGCTCTGCAATGTGAACTTGGGCTTTTTGCTACCAAGAT CTTGTTTAGAACTGAACATTTCCAAGTCTGTAACCAGAGAAGATGCTCCTCATTTTCTGAAGGAGCAGCA AAGAAAATCTGAAGAGTTTTCGACCTCTCATATGAAGTACAGTGGCCGAAGCATCAAGAGGCATAGTAGT GGGCTCAGGATATATGACAGGGAGGAGAAATTTCTCATCTCAAATGAAAAGAAGATATTTTCTGAAAATA GTTTAAAGTCTGAAGAACCTATCCTATGGACCAAGGGTGAGATTCTTGGAAAGGGAGCCTACGGCACAGT ATACTGTGGTCTCACTAGTCAAGGACAGCTAATAGCTGTAAAACAGGTGGCTTTGGATACCTCTAATAAA TTAGCTGCTGAAAAGGAATACCGGAAACTACAGGAAGAAGTAGATTTGCTCAAAGCACTGAAACATGTCA ACATTGTGGCCTATTTGGGGACATGCTTGCAAGAGAACACTGTGAGCATTTTCATGGAGTTTGTTCCTGG TGGCTCAATCTCTAGTATTATAAACCGTTTTGGGCCATTGCCTGAGATGGTGTTCTGTAAATATACGAAA CAAATACTTCAAGGTGTTGCTTATCTCCATGAGAACTGTGTGGTACATCGCGATATCAAAGGAAATAATG TTATGCTCATGCCAACTGGAATAATAAAGCTGATTGACTTTGGCTGTGCCAGGCGTTTGGCCTGGGCAGG TTTAAATGGCACCCACAGTGACATGCTTAAGTCCATGCATGGGACTCCATATTGGATGGCCCCAGAAGTC ATCAATGAGTCTGGCTATGGACGGAAATCAGATATCTGGAGCATTGGTTGTACTGTGTTTGAGATGGCTA CAGGGAAGCCTCCACTGGCTTCCATGGACAGGATGGCCGCCATGTTTTACATCGGAGCACACCGAGGGCT GATGCCTCCTTTACCAGACCACTTCTCAGAAAATGCAGCAGACTTTGTGCGCATGTGCCTGACCAGGGAC CAGCATGAGCGACCTTCTGCTCTCCAGCTCCTGAAGCACTCCTTCTTGGAGAGAAGTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219370 representing NM_001018046
Red=Cloning site Green=Tags(s) MSSMPKPERHAESLLDICHDTNSSPTDLMTVTKNQNIILQSISRSEEFDQDGDCSHSTLVNEEEDPSGGR QDWQPRTEGVEITVTFPRDVSPPQEMSQEDLKEKNLINSSLQEWAQAHAVSHPNEIETVELRKKKLTMRP LVLQKEESSRELCNVNLGFLLPRSCLELNISKSVTREDAPHFLKEQQRKSEEFSTSHMKYSGRSIKRHSS GLRIYDREEKFLISNEKKIFSENSLKSEEPILWTKGEILGKGAYGTVYCGLTSQGQLIAVKQVALDTSNK LAAEKEYRKLQEEVDLLKALKHVNIVAYLGTCLQENTVSIFMEFVPGGSISSIINRFGPLPEMVFCKYTK QILQGVAYLHENCVVHRDIKGNNVMLMPTGIIKLIDFGCARRLAWAGLNGTHSDMLKSMHGTPYWMAPEV INESGYGRKSDIWSIGCTVFEMATGKPPLASMDRMAAMFYIGAHRGLMPPLPDHFSENAADFVRMCLTRD QHERPSALQLLKHSFLERSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001018046 |
| ORF Size | 1530 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001018046.3 |
| RefSeq Size | 1711 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 80122 |
| UniProt ID | Q56UN5 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 57.5 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The protein kinase MAP3K19 phosphorylates MAP2Ks and thereby activates ERK and JNK kinases and increases viability of KRAS-mutant lung cancer cells
,Hoang, VT;Nyswaner, K;Torres-Ayuso, P;Brognard, J;,
J. Biol. Chem.
,PubMed ID 32358059
[MAP3K19]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219370L1 | Lenti ORF clone of Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2, Myc-DDK-tagged |
CNY 6,200.00 |
|
| RC219370L2 | Lenti ORF clone of Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC219370L3 | Lenti ORF clone of Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC219370L4 | Lenti ORF clone of Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG219370 | MAP3K19 (tGFP-tagged) - Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2 |
CNY 5,040.00 |
|
| SC302138 | MAP3K19 (untagged)-Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2 |
CNY 4,184.00 |
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