AGXT2 (NM_031900) Human Tagged ORF Clone
CAT#: RC219357
AGXT2 (Myc-DDK-tagged)-Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_031900" in other vectors (6)
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CNY 3,832.00
CNY 4,470.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AGT2; BAIBA; DAIBAT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219357 representing NM_031900
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTAATCTGGAGACATTTGCTGAGACCCTTGTGCCTGGTCACTTCCGCTCCCAGGATCCTTGAGA TGCATCCTTTCCTGAGCCTAGGTACTTCCCGGACATCAGTAACCAAGCTCAGTCTTCATACAAAGCCCAG AATGCCTCCATGTGACTTCATGCCTGAAAGATACCAGTCCCTTGGCTACAACCGTGTCCTGGAAATCCAC AAGGAACATCTTTCTCCTGTGGTGACGGCATATTTCCAGAAACCCCTGCTGCTCCACCAGGGGCACATGG AGTGGCTCTTTGATGCTGAAGGAAGCAGATACCTGGATTTCTTTTCCGGGATTGTTACTGTCAGTGTTGG CCATTGCCACCCAAAGGTGAATGCAGTGGCACAAAAGCAGCTCGGCCGCCTGTGGCATACAAGCACCGTC TTCTTCCACCCTCCAATGCATGAATATGCAGAGAAGCTTGCCGCACTTCTTCCTGAGCCTCTTAAGGTCA TTTTCTTGGTGAACAGTGGCTCAGAAGCCAATGAGCTGGCCATGCTGATGGCCAGGGCGCACTCAAACAA CATAGACATCATTTCTTTCAGAGGAGCCTACCATGGATGCAGTCCTTACACACTTGGCTTGACAAACGTA GGGATCTACAAGATGGAACTCCCTGGTGGGACAGGTTGCCAACCAACAATGTGTCCAGATGTTTTTCGTG GCCCTTGGGGAGGAAGCCACTGTCGAGATTCTCCAGTGCAAACAATCAGGAAGTGCAGCTGTGCACCAGA CTGCTGCCAAGCTAAAGATCAGTATATTGAGCAATTCAAAGATACGCTGAGCACATCTGTGGCCAAGTCA ATTGCTGGATTTTTCGCAGAACCTATTCAAGGTGTGAATGGAGTTGTCCAGTACCCAAAGGGGTTTCTAA AGGAAGCCTTTGAGCTGGTGCGAGCAAGGGGAGGCGTGTGCATTGCAGATGAAGTGCAGACAGGATTTGG AAGGTTGGGCTCTCACTTCTGGGGCTTCCAAACCCACGATGTCCTGCCTGACATTGTCACCATGGCTAAA GGGATTGGGAATGGCTTTCCCATGGCAGCAGTCATAACCACTCCAGAGATTGCCAAATCTTTGGCGAAAT GCCTGCAGCACTTCAACACCTTTGGAGGGAACCCCATGGCCTGTGCCATTGGATCTGCTGTGCTTGAGGT GATTAAAGAAGAAAATCTACAGGAAAACAGTCAAGAAGTTGGGACCTACATGTTACTAAAGTTTGCTAAG CTGCGGGATGAATTTGAAATTGTTGGAGACGTCCGAGGCAAAGGCCTCATGATAGGCATAGAAATGGTGC AGGATAAGATAAGCTGTCGGCCTCTTCCCCGTGAAGAAGTAAATCAGATCCATGAGGACTGCAAGCACAT GGGACTCCTCGTTGGCAGAGGCAGCATTTTTTCTCAGACATTTCGCATTGCGCCCTCAATGTGCATCACT AAACCAGAAGTTGATTTTGCAGTAGAAGTATTTCGTTCTGCCTTAACCCAACACATGGAAAGAAGAGCTA AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219357 representing NM_031900
Red=Cloning site Green=Tags(s) MTLIWRHLLRPLCLVTSAPRILEMHPFLSLGTSRTSVTKLSLHTKPRMPPCDFMPERYQSLGYNRVLEIH KEHLSPVVTAYFQKPLLLHQGHMEWLFDAEGSRYLDFFSGIVTVSVGHCHPKVNAVAQKQLGRLWHTSTV FFHPPMHEYAEKLAALLPEPLKVIFLVNSGSEANELAMLMARAHSNNIDIISFRGAYHGCSPYTLGLTNV GIYKMELPGGTGCQPTMCPDVFRGPWGGSHCRDSPVQTIRKCSCAPDCCQAKDQYIEQFKDTLSTSVAKS IAGFFAEPIQGVNGVVQYPKGFLKEAFELVRARGGVCIADEVQTGFGRLGSHFWGFQTHDVLPDIVTMAK GIGNGFPMAAVITTPEIAKSLAKCLQHFNTFGGNPMACAIGSAVLEVIKEENLQENSQEVGTYMLLKFAK LRDEFEIVGDVRGKGLMIGIEMVQDKISCRPLPREEVNQIHEDCKHMGLLVGRGSIFSQTFRIAPSMCIT KPEVDFAVEVFRSALTQHMERRAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_031900 |
ORF Size | 1542 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031900.4 |
RefSeq Size | 2165 bp |
RefSeq ORF | 1545 bp |
Locus ID | 64902 |
UniProt ID | Q9BYV1 |
Protein Families | Druggable Genome |
Protein Pathways | Alanine, aspartate and glutamate metabolism |
MW | 52.4 kDa |
Gene Summary | The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. It is an important regulator of methylarginines and is involved in the control of blood pressure in kidney. Polymorphisms in this gene affect methylarginine and beta-aminoisobutyrate metabolism, and are associated with carotid atherosclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219357L1 | Lenti ORF clone of Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,232.00 |
|
RC219357L2 | Lenti ORF clone of Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,370.00 |
|
RC219357L3 | Lenti ORF clone of Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,370.00 |
|
RC219357L4 | Lenti ORF clone of Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,370.00 |
|
RG219357 | AGXT2 (tGFP-tagged) - Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein |
CNY 5,432.00 |
|
SC305360 | AGXT2 (untagged)-Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein |
CNY 3,832.00 |