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AMN (NM_030943) Human Tagged ORF Clone
CAT＃: RC219248
- TrueORF®
AMN (Myc-DDK-tagged)-Human amnionless homolog (mouse) (AMN)
ORF Plasmid: tGFP
"NM_030943" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | amnionless; IGS2; PRO1028 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219248 representing NM_030943
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGTCCTGGGCCGGGTCCTGCTGTGGCTGCAGCTCTGCGCACTGACCCAGGCGGTCTCCAAACTCT GGGTCCCCAACACGGACTTCGACGTCGCAGCCAACTGGAGCCAGAACCGGACCCCGTGCGCCGGCGGCGC CGTTGAGTTCCCGGCGGACAAGATGGTGTCAGTCCTGGTGCAAGAAGGTCACGCCGTCTCAGACATGCTC CTGCCGCTGGATGGGGAACTCGTCCTGGCTTCAGGAGCCGGATTCGGCGTCTCAGACGTGGGCTCGCACC TGGACTGTGGCGCGGGCGAACCTGCCGTCTTCCGCGACTCTGACCGCTTCTCCTGGCATGACCCGCACCT GTGGCGCTCTGGGGACGAGGCACCTGGCCTCTTCTTCGTGGACGCCGAGCGCGTGCCCTGCCGCCACGAC GACGTCTTCTTTCCGCCTAGTGCCTCCTTCCGCGTGGGGCTCGGCCCTGGCGCTAGCCCCGTGCGTGTCC GCAGCATCTCGGCTCTGGGCCGGACGTTCACGCGCGACGAGGACCTGGCTGTTTTCCTGGCGTCCCGCGC GGGCCGCCTACGCTTCCACGGGCCGGGCGCGCTGAGCGTGGGCCCCGAGGACTGCGCGGACCCGTCGGGC TGCGTCTGCGGCAACGCGGAGGCGCAGCCGTGGATCTGCGCGGCCCTGCTCCAGCCCCTGGGCGGCCGCT GTCCCCAGGCCGCCTGCCACTTTGCCCTCCGGCCCCAGGGGCAGTGCTGTGACCTCTGTGGAGCCGTTGT GTTGCTGACCCACGGCCCCGCATTTGACCTGGAGCGGTACCGGGCGCGGATACTGGACACCTTCCTGGGT CTGCCTCAGTACCACGGGCTGCAGGTGGCCGTGTCCAAGGTGCCACGCTCGTCCCGGCTCCGTGAGGCCG ATACGGAGATCCAGGTGGTGCTGGTGGAGAATGGGCCCGAGACAGGCGGAGCGGGGCGGCTGGCCCGGGC CCTCCTGGCGGACGTCGCCGAGAACGGCGAGGCCCTCGGCGTCCTGGAGGCGACCATGCGGGAGTCGGGC GCACACGTCTGGGGCAGCTCCGCGGCTGGGCTGGCGGGCGGCGTGGCGGCTGCCGTGCTGCTGGCGCTGC TGGTCCTGCTGGTGGCGCCGCCGCTGCTGCGCCGCGCGGGGAGGCTCAGGTGGAGGAGGCACGAGGCGGC GGCCCCGGCTGGAGCGCCCCTCGGCTTCCGCAACCCGGTGTTCGACGTGACGGCCTCCGAGGAGCTGCCC CTGCCGCGGCGGCTCAGCCTGGTTCCGAAGGCGGCCGCAGACAGCACCAGCCACAGTTACTTCGTCAACC CTCTGTTCGCCGGGGCCGAGGCCGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219248 representing NM_030943
Red=Cloning site Green=Tags(s) MGVLGRVLLWLQLCALTQAVSKLWVPNTDFDVAANWSQNRTPCAGGAVEFPADKMVSVLVQEGHAVSDML LPLDGELVLASGAGFGVSDVGSHLDCGAGEPAVFRDSDRFSWHDPHLWRSGDEAPGLFFVDAERVPCRHD DVFFPPSASFRVGLGPGASPVRVRSISALGRTFTRDEDLAVFLASRAGRLRFHGPGALSVGPEDCADPSG CVCGNAEAQPWICAALLQPLGGRCPQAACHFALRPQGQCCDLCGAVVLLTHGPAFDLERYRARILDTFLG LPQYHGLQVAVSKVPRSSRLREADTEIQVVLVENGPETGGAGRLARALLADVAENGEALGVLEATMRESG AHVWGSSAAGLAGGVAAAVLLALLVLLVAPPLLRRAGRLRWRRHEAAAPAGAPLGFRNPVFDVTASEELP LPRRLSLVPKAAADSTSHSYFVNPLFAGAEAEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030943 |
| ORF Size | 1359 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030943.1, NP_112205.1 |
| RefSeq Size | 1896 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 81693 |
| UniProt ID | Q9BXJ7 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 45.6 kDa |
| Gene Summary | The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells
,Udagawa, T;Harita, Y;Miura, K;Mitsui, J;Ode, KL;Morishita, S;Urae, S;Kanda, S;Kajiho, Y;Tsurumi, H;Ueda, HR;Tsuji, S;Saito, A;Oka, A;,
Sci Rep
,PubMed ID 29402915
[AMN]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219248L1 | Lenti ORF clone of Human amnionless homolog (mouse) (AMN), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC219248L2 | Lenti ORF clone of Human amnionless homolog (mouse) (AMN), mGFP tagged |
CNY 5,890.00 |
|
| RC219248L3 | Lenti ORF clone of Human amnionless homolog (mouse) (AMN), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219248L4 | Lenti ORF clone of Human amnionless homolog (mouse) (AMN), mGFP tagged |
CNY 5,890.00 |
|
| RG219248 | AMN (tGFP-tagged) - Human amnionless homolog (mouse) (AMN) |
CNY 5,256.00 |
|
| SC305303 | AMN (untagged)-Human amnionless homolog (mouse) (AMN) |
CNY 3,656.00 |
