NANOS1 (NM_199461) Human Tagged ORF Clone
CAT#: RC219123
NANOS1 (Myc-DDK-tagged)-Human nanos homolog 1 (Drosophila) (NANOS1)
ORF Plasmid: tGFP
"NM_199461" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EC_Rep1a; NOS-1; NOS1; SPGF12; ZC2HC12A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219123 representing NM_199461
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTTTCCCCTGGGCGCCCCGCTCGCCCCGCCGCGGCCGCGCCCCCCCGCCCATGGCGCTCGTGC CCAGCGCCCGCTACGTGAGCGCCCCGGGCCCGGCGCACCCGCAGCCCTTCAGCTCCTGGAACGACTACCT GGGGCTCGCCACGCTCATCACCAAAGCGGTGGACGGCGAGCCGCGCTTCGGCTGCGCCCGCGGTGGGAAC GGCGGCGGCGGCTCCCCGCCCTCCTCCTCCTCGTCGTCCTGCTGCTCCCCCCACACGGGGGCCGGGCCTG GGGCGCTGGGGCCGGCGCTGGGGCCGCCCGACTACGACGAGGACGACGACGACGACAGCGACGAGCCGGG GTCCCGGGGCCGCTACCTGGGGAGCGCGCTGGAATTGCGCGCGCTGGAGCTGTGCGCGGGCCCCGCCGAG GCCGGGCTGCTGGAGGAGCGCTTCGCCGAGCTGAGCCCGTTCGCGGGTCGTGCCGCCGCCGTGCTGCTGG GCTGCGCGCCCGCCGCCGCCGCCGCCGCCACCACCACCAGCGAGGCGACGCCGCGCGAGGAGCGGGCCCC GGCGTGGGCGGCCGAGCCCCGGCTGCACGCGGCCTCCGGGGCGGCGGCCGCCCGGCTGCTGAAGCCCGAG CTGCAGGTGTGCGTGTTCTGCCGGAACAACAAGGAGGCGATGGCGCTCTACACCACCCATATCCTCAAGG GCCCCGACGGGCGAGTGCTGTGTCCCGTGCTGCGCCGCTACACGTGTCCCCTGTGCGGCGCCAGCGGCGA CAACGCGCACACCATCAAGTACTGCCCGCTCTCCAAAGTGCCGCCGCCGCCCGCCCGCCCGCCGCCCCGC AGCGCCAGGGACGGCCCGCCTGGCAAGAAGCTGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219123 representing NM_199461
Red=Cloning site Green=Tags(s) MEAFPWAPRSPRRGRAPPPMALVPSARYVSAPGPAHPQPFSSWNDYLGLATLITKAVDGEPRFGCARGGN GGGGSPPSSSSSSCCSPHTGAGPGALGPALGPPDYDEDDDDDSDEPGSRGRYLGSALELRALELCAGPAE AGLLEERFAELSPFAGRAAAVLLGCAPAAAAAATTTSEATPREERAPAWAAEPRLHAASGAAAARLLKPE LQVCVFCRNNKEAMALYTTHILKGPDGRVLCPVLRRYTCPLCGASGDNAHTIKYCPLSKVPPPPARPPPR SARDGPPGKKLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199461 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199461.4 |
RefSeq Size | 4627 bp |
RefSeq ORF | 879 bp |
Locus ID | 340719 |
UniProt ID | Q8WY41 |
MW | 30 kDa |
Gene Summary | This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model
,null,
International Journal of Molecular Sciences
,PubMed ID 35743036
[NANOS1]
|
Human NANOS1 Represses Apoptosis by Downregulating Pro-Apoptotic Genes in the Male Germ Cell Line
,Janecki, DM;Ilaslan, E;Smialek, MJ;Sajek, MP;Kotecki, M;Ginter-Matuszewska, B;Krainski, P;Jaruzelska, J;Kusz-Zamelczyk, K;,
Int J Mol Sci
,PubMed ID 32344590
[NANOS1]
|
A male infertility mutation reverts NANOS1 activity from anti-apoptotic to pro-apoptotic by disrupting repression of GADD45A, GADD45B, GADD45G and RHOB genes
,Janecki, DM;Ilaslan, E;Smialek, MJ;Sajek, MP;,
bioRxiv
[NANOS1]
|
A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
,Amadei, G;Zander, MA;Yang, G;Dumelie, JG;Vessey, JP;Lipshitz, HD;Smibert, CA;Kaplan, DR;Miller, FD;,
J. Neurosci.
,PubMed ID 26609159
[NANOS1]
|
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219123L1 | Lenti ORF clone of Human nanos homolog 1 (Drosophila) (NANOS1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC219123L2 | Lenti ORF clone of Human nanos homolog 1 (Drosophila) (NANOS1), mGFP tagged |
CNY 5,890.00 |
|
RC219123L3 | Lenti ORF clone of Human nanos homolog 1 (Drosophila) (NANOS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC219123L4 | Lenti ORF clone of Human nanos homolog 1 (Drosophila) (NANOS1), mGFP tagged |
CNY 5,890.00 |
|
RG219123 | NANOS1 (tGFP-tagged) - Human nanos homolog 1 (Drosophila) (NANOS1) |
CNY 4,370.00 |
|
SC307986 | NANOS1 (untagged)-Human nanos homolog 1 (Drosophila) (NANOS1) |
CNY 2,400.00 |