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CALM1 (NM_006888) Human Tagged ORF Clone
CAT＃: RC219086
CALM1 (Myc-DDK-tagged)-Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1
ORF Plasmid: tGFP
"NM_006888" in other vectors (6)
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CNY 1,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 3,280.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CALML2; caM; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; CPVT4; DD132; LQT14; PHKD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219086 representing NM_006888
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGATCAGCTGACCGAAGAACAGATTGCTGAATTCAAGGAAGCCTTCTCCCTATTTGATAAAGATG GCGATGGCACCATCACAACAAAGGAACTTGGAACTGTCATGAGGTCACTGGGTCAGAACCCAACAGAAGC TGAATTGCAGGATATGATCAATGAAGTGGATGCTGATGGTAATGGCACCATTGACTTCCCCGAATTTTTG ACTATGATGGCTAGAAAAATGAAAGATACAGATAGTGAAGAAGAAATCCGTGAGGCATTCCGAGTCTTTG ACAAGGATGGCAATGGTTATATCAGTGCAGCAGAACTACGTCACGTCATGACAAACTTAGGAGAAAAACT AACAGATGAAGAAGTAGATGAAATGATCAGAGAAGCAGATATTGATGGAGACGGACAAGTCAACTATGAA GAATTCGTACAGATGATGACTGCAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219086 representing NM_006888
Red=Cloning site Green=Tags(s) MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFL TMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYE EFVQMMTAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006888 |
| ORF Size | 447 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006888.6 |
| RefSeq Size | 3718 bp |
| RefSeq ORF | 450 bp |
| Locus ID | 801 |
| UniProt ID | P62158 |
| Domains | EFh |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction |
| MW | 16.7 kDa |
| Gene Summary | This gene encodes one of three calmodulin proteins which are members of the EF-hand calcium-binding protein family. Calcium-induced activation of calmodulin regulates and modulates the function of cardiac ion channels. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.A missense mutation in the CALM1 gene has been associated with ventricular tachycardia.[provided by RefSeq, May 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219086L1 | Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC219086L2 | Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC219086L3 | Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219086L4 | Lenti ORF clone of Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG219086 | CALM1 (tGFP-tagged) - Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1 |
CNY 3,400.00 |
|
| SC115829 | CALM1 (untagged)-Human calmodulin 1 (phosphorylase kinase, delta) (CALM1), transcript variant 1 |
CNY 1,200.00 |
