Endostatin (COL18A1) (NM_030582) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GLCC; KNO; KNO1; KS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC219034 representing NM_030582 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCTACCCCTGTGGCTGCCACATCCTGCTGCTGCTCTTCTGCTGCCTGGCGGCTGCCCGGGCCA ACCTGCTGAACCTGAACTGGCTTTGGTTCAATAATGAGGACACCAGCCATGCAGCTACCACGATCCCTGA GCCCCAGGGGCCCCTGCCTGTGCAGCCCACAGCAGATACCACCACACACGTGACCCCCCGGAATGGTTCC ACAGAGCCAGCGACAGCCCCTGGCAGCCCTGAGCCACCCTCAGAGCTGCTGGAAGATGGCCAGGACACCC CCACTTCTGCCGAGAGCCCGGACGCGCCAGAGGAGAACATTGCCGGTGTCGGAGCCGAGATCCTGAACGT GGCCAAAGGCATCCGGAGCTTCGTCCAGCTGTGGAATGACACTGTCCCCACTGAGAGCTTGGCCAGGGCG GAAACCCTGGTCCTGGAGACTCCTGTGGGCCCCCTTGCCCTCGCTGGGCCTTCCAGCACCCCCCAGGAGA ATGGGACCACTCTCTGGCCCAGCCGTGGCATTCCTAGCTCTCCGGGCGCCCACACAACCGAGGCTGGCAC CTTGCCTGCACCCACCCCATCGCCTCCCTCCCTGGGCAGGCCCTGGGCACCACTCACGGGGCCCTCAGTG CCACCACCATCTTCAGAGCGCATCAGCGAGGAGGTGGGGCTGCTGCAGCTCCTTGGGGACCCCCCGCCCC AGCAGGTCACCCAGACGGATGACCCCGACGTCGGGCTGGCCTACGTCTTTGGGCCAGATGCCAACAGTGG CCAAGTGGCCCGGTACCACTTCCCCAGCCTCTTCTTCCGTGACTTCTCACTGCTGTTCCACATCCGGCCA GCCACAGAGGGCCCAGGGGTGCTGTTCGCCATCACGGACTCGGCGCAGGCCATGGTCTTGCTGGGCGTGA AGCTCTCTGGGGTGCAGGACGGGCACCAGGACATCTCCCTGCTCTACACAGAACCAGGTGCAGGCCAGAC CCACACAGCCGCCAGCTTCCGGCTCCCCGCCTTCGTCGGCCAGTGGACACACTTAGCCCTCAGTGTGGCA GGTGGCTTTGTGGCCCTCTACGTGGACTGTGAGGAGTTCCAGAGAATGCCGCTTGCTCGGTCCTCACGGG GCCTGGAGCTGGAGCCTGGCGCCGGGCTCTTCGTGGCTCAGGCGGGGGGAGCGGACCCTGACAAGTTCCA GGGGGTGATCGCTGAGCTGAAGGTGCGCAGGGACCCCCAGGTGAGCCCCATGCACTGCCTGGACGAGGAA GGCGATGACTCAGATGGGGCATCCGGAGACTCTGGCAGCGGGCTCGGGGACGCCCGGGAGCTTCTCAGGG AGGAGACGGGCGCGGCCCTAAAACCCAGGCTCCCCGCGCCACCCCCCGTCACCACGCCACCCTTGGCTGG AGGCAGCAGCACGGAAGATTCCAGAAGTGAAGAAGTCGAGGAGCAGACCACGGTGGCTTCGTTAGGAGCT CAGACACTTCCTGGCTCAGATTCTGTCTCCACGTGGGACGGGAGTGTCCGGACCCCTGGGGGCCGCGTGA AAGAGGGCGGCCTGAAGGGGCAGAAAGGGGAGCCAGGTGTTCCGGGCCCACCTGGCCGGGCAGGCCCCCC AGGATCCCCATGCCTACCTGGTCCCCCGGGTCTCCCGTGCCCAGTGAGTCCCCTGGGTCCTGCAGGCCCA GCGTTGCAAACTGTCCCCGGACCACAAGGACCCCCAGGGCCTCCGGGGAGGGACGGCACCCCTGGAAGGG ACGGCGAGCCGGGCGACCCCGGTGAAGACGGAAAGCCGGGCGACACCGGGCCACAAGGCTTCCCCGGGAC TCCAGGGGACGTAGGTCCCAAGGGCGACAAGGGAGACCCTGGGGTTGGAGAGAGAGGGCCCCCAGGACCC CAAGGGCCTCCAGGGCCCCCAGGACCCTCCTTCAGACACGACAAGCTGACCTTCATTGACATGGAGGGAT CTGGCTTCGGGGGCGATCTGGAGGCCCTGCGGGGTCCTCGAGGCTTCCCTGGACCTCCCGGACCCCCCGG TGTCCCAGGCCTGCCCGGCGAGCCAGGCCGCTTTGGGGTGAACAGCTCCGACGTCCCAGGACCCGCCGGC CTTCCTGGTGTGCCTGGGCGCGAGGGTCCCCCCGGGTTTCCTGGCCTCCCGGGACCCCCAGGCCCTCCGG GAAGAGAGGGGCCCCCAGGAAGGACTGGGCAGAAAGGCAGCCTGGGTGAAGCAGGCGCCCCAGGACATAA GGGGAGCAAGGGAGCCCCCGGTCCTGCTGGTGCTCGTGGGGAGAGCGGCCTGGCAGGAGCCCCCGGACCT GCTGGACCACCAGGCCCCCCTGGGCCCCCTGGGCCCCCAGGACCAGGACTCCCCGCTGGATTTGATGACA TGGAAGGCTCCGGGGGGCCCTTCTGGTCAACAGCCCGAAGCGCTGATGGGCCACAGGGACCTCCCGGCCT GCCGGGACTTAAGGGGGATCCTGGCGTGCCTGGGCTGCCGGGGGCGAAGGGAGAAGTTGGAGCAGATGGA GTCCCCGGGTTCCCCGGCCTCCCTGGCAGAGAGGGCATTGCTGGGCCCCAGGGGCCAAAGGGAGACAGAG GCAGCCGGGGAGAAAAGGGAGATCCAGGGAAGGACGGAGTCGGGCAGCCGGGCCTCCCTGGCCCCCCCGG ACCCCCGGGACCTGTGGTCTACGTGTCGGAGCAGGACGGATCCGTCCTGAGCGTGCCGGGACCTGAGGGC CGGCCGGGTTTCGCAGGCTTTCCCGGACCTGCAGGACCCAAGGGCAACCTGGGCTCTAAGGGCGAACGAG GCTCCCCGGGACCCAAGGGTGAGAAGGGTGAACCGGGCAGCATCTTCAGCCCCGACGGCGGTGCCCTGGG CCCTGCCCAGAAAGGAGCCAAGGGAGAGCCGGGCTTCCGAGGACCCCCGGGTCCATACGGACGGCCGGGG TACAAGGGAGAGATTGGCTTTCCTGGACGGCCGGGTCGCCCCGGGATGAACGGATTGAAAGGAGAGAAAG GGGAGCCGGGAGATGCCAGCCTTGGATTTGGCATGAGGGGAATGCCCGGCCCCCCAGGACCTCCAGGGCC CCCAGGCCCTCCAGGGACTCCTGTTTACGACAGCAATGTGTTTGCTGAGTCCAGCCGCCCCGGGCCTCCA GGATTGCCAGGGAATCAGGGCCCTCCAGGACCCAAGGGCGCCAAAGGAGAAGTGGGCCCCCCCGGACCAC CAGGGCAGTTTCCGTTTGACTTTCTTCAGTTGGAGGCTGAAATGAAGGGGGAGAAGGGAGACCGAGGTGA TGCAGGACAGAAAGGCGAAAGGGGGGAGCCCGGGGGCGGCGGTTTCTTCGGCTCCAGCCTGCCCGGCCCC CCCGGCCCCCCAGGCCCACGTGGCTACCCTGGGATTCCAGGTCCCAAGGGAGAGAGCATCCGGGGCCAGC CCGGCCCACCTGGACCTCAGGGACCCCCCGGCATCGGCTACGAGGGGCGCCAGGGCCCTCCCGGCCCCCC AGGCCCCCCAGGGCCCCCTTCATTTCCTGGCCCTCACAGGCAGACTATCAGCGTTCCCGGCCCTCCGGGC CCCCCTGGGCCCCCTGGGCCCCCTGGAACCATGGGCGCCTCCTCAGGGGTGAGGCTCTGGGCTACACGCC AGGCCATGCTGGGCCAGGTGCACGAGGTTCCCGAGGGCTGGCTCATCTTCGTGGCCGAGCAGGAGGAGCT CTACGTCCGCGTGCAGAACGGGTTCCGGAAGGTCCAGCTGGAGGCCCGGACACCACTCCCACGAGGGACG GACAATGAAGTGGCCGCCTTGCAGCCCCCCGTGGTGCAGCTGCACGACAGCAACCCCTACCCGCGGCGGG AGCACCCCCACCCCACCGCGCGGCCCTGGCGGGCAGATGACATCCTGGCCAGCCCCCCTCGCCTGCCCGA GCCCCAGCCCTACCCCGGAGCCCCGCACCACAGCTCCTACGTGCACCTGCGGCCGGCGCGACCCACAAGC CCACCCGCCCACAGCCACCGCGACTTCCAGCCGGTGCTCCACCTGGTTGCGCTCAACAGCCCCCTGTCAG GCGGCATGCGGGGCATCCGCGGGGCCGACTTCCAGTGCTTCCAGCAGGCGCGGGCCGTGGGGCTGGCGGG CACCTTCCGCGCCTTCCTGTCCTCGCGCCTGCAGGACCTGTACAGCATCGTGCGCCGTGCCGACCGCGCA GCCGTGCCCATCGTCAACCTCAAGGACGAGCTGCTGTTTCCCAGCTGGGAGGCTCTGTTCTCAGGCTCTG AGGGTCCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGACGGCAAGGACGTCCTGAGGCACCCCACCTG GCCCCAGAAGAGCGTGTGGCATGGCTCGGACCCCAACGGGCGCAGGCTGACCGAGAGCTACTGTGAGACG TGGCGGACGGAGGCTCCCTCGGCCACGGGCCAGGCCTCCTCGCTGCTGGGGGGCAGGCTCCTGGGGCAGA GTGCCGCGAGCTGCCATCACGCCTACATCGTGCTCTGCATTGAGAACAGCTTCATGACTGCCTCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219034 representing NM_030582 Red=Cloning site Green=Tags(s) MAPYPCGCHILLLLFCCLAAARANLLNLNWLWFNNEDTSHAATTIPEPQGPLPVQPTADTTTHVTPRNGS TEPATAPGSPEPPSELLEDGQDTPTSAESPDAPEENIAGVGAEILNVAKGIRSFVQLWNDTVPTESLARA ETLVLETPVGPLALAGPSSTPQENGTTLWPSRGIPSSPGAHTTEAGTLPAPTPSPPSLGRPWAPLTGPSV PPPSSERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVFGPDANSGQVARYHFPSLFFRDFSLLFHIRP ATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYTEPGAGQTHTAASFRLPAFVGQWTHLALSVA GGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGGADPDKFQGVIAELKVRRDPQVSPMHCLDEE GDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPVTTPPLAGGSSTEDSRSEEVEEQTTVASLGA QTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGPPGRAGPPGSPCLPGPPGLPCPVSPLGPAGP ALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGDKGDPGVGERGPPGP QGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFPGPPGPPGVPGLPGEPGRFGVNSSDVPGPAG LPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGEAGAPGHKGSKGAPGPAGARGESGLAGAPGP AGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADGPQGPPGLPGLKGDPGVPGLPGAKGEVGADG VPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDGSVLSVPGPEG RPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFSPDGGALGPAQKGAKGEPGFRGPPGPYGRPG YKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPGPPGPPGPPGPPGTPVYDSNVFAESSRPGPP GLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKGEKGDRGDAGQKGERGEPGGGGFFGSSLPGP PGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTISVPGPPG PPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPLPRGT DNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILASPPRLPEPQPYPGAPHHSSYVHLRPARPTS PPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRA AVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCET WRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_030582
ORF Size	4548 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_030582.4
RefSeq Size	5910 bp
RefSeq ORF	4551 bp
Locus ID	80781
UniProt ID	P39060
MW	153.8 kDa
Gene Summary	This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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