ELAVL2 (NM_004432) Human Tagged ORF Clone

CAT#: RC218975

  • TrueORF®

ELAVL2 (Myc-DDK-tagged)-Human ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) (ELAVL2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_004432" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


HuB/HuD Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HEL-N1; HELN1; HUB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218975 representing NM_004432
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACACAACTGTCTAATGGGCCAACTTGCAATAACACAGCCAATGGTCCAACCACCATAAACAACA
ACTGTTCGTCACCAGTTGACTCTGGGAACACAGAAGACAGCAAGACCAACTTAATAGTCAACTACCTTCC
TCAGAACATGACACAGGAGGAACTAAAGAGTCTCTTTGGGAGCATTGGTGAAATAGAGTCCTGTAAGCTT
GTAAGAGACAAAATAACAGGGCAGAGCTTGGGATATGGCTTTGTGAACTACATTGACCCCAAGGATGCAG
AGAAAGCTATCAACACCCTGAATGGATTGAGACTTCAAACCAAAACAATAAAAGTTTCCTATGCTCGCCC
AAGTTCAGCTTCTATCAGAGATGCAAATTTATATGTCAGCGGACTTCCAAAAACAATGACCCAGAAGGAG
TTGGAACAGCTTTTTTCACAATATGGACGCATTATTACTTCTCGTATTCTTGTCGACCAGGTCACTGGCA
TATCAAGGGGTGTAGGGTTTATTCGATTTGACAAGCGAATTGAGGCAGAAGAAGCTATCAAAGGCCTAAA
TGGCCAGAAACCTCCCGGTGCCACGGAGCCAATCACTGTAAAGTTTGCTAATAACCCAAGCCAAAAAACC
AATCAGGCCATCCTTTCCCAGCTGTACCAGTCTCCAAACAGAAGGTATCCAGGACCGCTAGCTCAGCAGG
CACAGCGTTTTAGGTTGGACAATCTGCTCAATATGGCTTATGGAGTAAAGAGGTTTTCTCCAATGACCAT
TGACGGAATGACCAGTTTGGCTGGAATTAATATCCCTGGGCACCCTGGAACAGGGTGGTGTATATTTGTG
TACAACCTGGCTCCTGACGCAGATGAGAGTATCCTGTGGCAAATGTTTGGGCCTTTTGGAGCTGTCACCA
ATGTGAAGGTCATCCGTGACTTTAACACCAATAAATGCAAAGGTTTTGGATTTGTGACTATGACAAACTA
TGATGAGGCTGCCATGGCGATACGTAGCCTCAATGGATACCGTCTGGGAGACAGAGTACTGCAGGTCTCC
TTTAAGACAAACAAAACGCACAAAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218975 representing NM_004432
Red=Cloning site Green=Tags(s)

METQLSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKL
VRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKE
LEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKT
NQAILSQLYQSPNRRYPGPLAQQAQRFRLDNLLNMAYGVKRFSPMTIDGMTSLAGINIPGHPGTGWCIFV
YNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIRSLNGYRLGDRVLQVS
FKTNKTHKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004432
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004432.1, NP_004423.1
RefSeq Size 2233 bp
RefSeq ORF 1080 bp
Locus ID 1993
UniProt ID Q12926
Domains RRM
Protein Families Transcription Factors
MW 39.3 kDa
Gene Summary In humans, the ELAV like RNA binding protein gene family has four members (ELAVL1-4). ELAVL RNA binding proteins recognize AU-rich elements in the 3' UTRs of gene transcripts and thereby regulate gene expression post-transcriptionally. The protein encoded by this gene binds to several 3' UTRs, including its own and also that of FOS, ID, and POU5F1. This gene encodes ELAVL2 and, like ELAVL3 and ELAVL4, is expressed specifically in neurons and primarily localizes to the cytoplasm. This protein also forms a cytosolic complex with the normally nuclear-localized ELAVL1 protein. Alternative splicing of this gene results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Jul 2020]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...