MYO5A (NM_000259) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GS1; MYH12; MYO5; MYR12
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC218817 representing NM_000259. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGCGTCGGAGCTCTACACAAAGTTTGCCAGGGTTTGGATACCTGATCCAGAGGAAGTCTGGAAG TCAGCAGAGCTGCTCAAAGATTATAAGCCAGGAGATAAAGTCCTCCTGCTTCACCTCGAGGAAGGAAAG GATTTGGAATACCATCTAGATCCAAAGACCAAGGAGCTGCCTCACTTACGAAATCCTGACATACTTGTT GGTGAAAATGACCTCACAGCCCTCAGCTATCTTCATGAGCCTGCTGTGCTCCATAATCTCAGAGTCCGC TTTATTGATTCCAAACTTATTTATACGTATTGTGGTATAGTCCTAGTAGCTATAAATCCCTATGAACAG CTGCCTATTTATGGAGAAGATATTATTAATGCATACAGTGGTCAGAACATGGGTGATATGGATCCACAT ATCTTTGCAGTAGCTGAAGAAGCTTACAAGCAAATGGCCAGAGATGAACGAAATCAGTCCATCATCGTA AGTGGAGAGTCTGGGGCAGGAAAAACAGTCTCAGCTAAGTATGCCATGCGATACTTTGCAACTGTGAGT GGTTCTGCCAGTGAGGCCAATGTGGAGGAAAAGGTCTTGACCTCCAACCCCATCATGGAGTCCATTGGA AATGCTAAAACAACCAGGAATGATAATAGCAGCCGTTTTGGGAAGTATATTGAGATTGGTTTTGATAAG AGATATCGAATCATTGGTGCCAATATGAGAACTTATCTTTTAGAGAAATCCAGAGTGGTATTCCAGGCA GAAGAGGAGAGAAACTATCATATCTTCTATCAGCTTTGTGCCTCAGCAAAGTTACCTGAATTTAAAATG CTACGATTAGGAAATGCAGATAACTTTAATTACACAAAACAAGGAGGCAGTCCTGTGATTGAAGGAGTG GATGATGCAAAGGAGATGGCACATACTAGGCAGGCCTGCACTTTGCTAGGAATTAGTGAATCTCATCAA ATGGGAATTTTCCGAATACTTGCTGGCATCCTTCACTTAGGCAATGTTGGATTTACATCCCGAGATGCA GACAGCTGCACAATACCTCCCAAGCATGAACCTCTCTGCATCTTCTGTGACCTCATGGGTGTGGACTAT GAGGAGATGTGTCACTGGCTCTGCCATCGGAAACTGGCTACTGCCACAGAGACATACATCAAGCCCATC TCCAAGCTGCAGGCCACGAATGCCCGCGATGCTTTGGCCAAACACATCTATGCCAAGCTCTTTAACTGG ATTGTAGATAATGTCAATCAGGCTCTCCATTCTGCTGTCAAACAGCACTCTTTTATTGGTGTGCTAGAC ATTTACGGATTTGAAACATTTGAGATAAATAGTTTTGAACAGTTTTGCATAAATTATGCAAATGAAAAA CTACAGCAACAATTCAATATGCATGTCTTCAAATTGGAGCAAGAAGAATATATGAAGGAACAAATTCCA TGGACACTCATAGATTTTTATGATAATCAGCCTTGTATTAATCTTATAGAATCAAAACTAGGCATTCTA GATTTACTGGATGAGGAATGCAAGATGCCTAAAGGCACAGATGACACCTGGGCCCAAAAATTGTACAAC ACACATTTGAACAAATGTGCACTCTTTGAAAAGCCTCGTCTATCAAACAAAGCTTTCATCATCCAACAT TTTGCTGACAAAGTGGAATACCAGTGTGAAGGATTTCTCGAAAAGAATAAAGACACCGTTTTTGAAGAA CAAATTAAAGTTCTTAAATCAAGCAAGTTTAAGATGCTACCAGAACTATTTCAAGATGATGAGAAGGCC ATCAGTCCAACTTCAGCCACCTCCTCAGGGCGCACACCCCTCACACGAACTCCTGCAAAGCCCACCAAA GGCAGACCAGGCCAAATGGCCAAAGAGCACAAGAAAACAGTGGGGCATCAGTTCAGAAACTCCCTGCAC CTGCTTATGGAGACACTCAATGCCACTACCCCTCACTATGTGCGCTGTATCAAGCCTAATGACTTCAAG CTCCCATTCACGTTTGATGAGAAGAGGGCAGTGCAGCAGCTGAGAGCATGTGGTGTCCTGGAAACCATC CGAATCAGTGCGGCCGGTTTCCCCTCACGGTGGACTTACCAAGAATTTTTCAGCCGCTACCGTGTCCTA ATGAAGCAGAAAGATGTGCTGAGTGACAGAAAGCAAACATGCAAGAATGTGTTAGAGAAACTGATACTG GACAAGGACAAATACCAGTTTGGTAAGACAAAGATCTTTTTCCGTGCCGGTCAAGTGGCCTATCTAGAA AAATTGAGAGCTGACAAACTGAGAGCTGCCTGCATCCGGATCCAGAAGACCATCCGAGGGTGGCTGCTG AGAAAGAAGTACCTACGCATGCGGAAGGCAGCCATCACCATGCAGAGATACGTGCGGGGCTACCAGGCC CGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGCAGCAACCATCATTCAAAAGTACTGGCGCATGTAT GTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCCACTATTGTTCTTCAGTCTTACTTGCGAGGCTTC TTGGCCAGAAATAGGTATCGCAAGATACTCCGTGGGCACAAAGCAGTCATCATTCAGAAGCGAGTCCGG GGCTGGCTGGCCCGCACACACTACAAGAGGAGCATGCATGCCATCATCTACCTTCAGTGCTGCTTCAGG CGGATGATGGCCAAGCGTGAGCTAAAGAAGCTCAAAATCGAGGCTCGCTCAGTGGAGCGCTATAAGAAG CTGCGCATCGGCATGGAGAACAAGATCATGCAGCTGCAGCGCAAAGTTGATGAGCAGAACAAAGACTAC AAATGCCTTGTGGAGAAACTAACCAATCTGGAAGGAATATACAACTCTGAGACTGAGAAACTACGAAGT GACTTAGAACGTCTTCAACTAAGTGAAGAGGAAGCGAAAGTTGCCACTGGGCGGGTCCTTAGTCTGCAG GAGGAAATTGCCAAGCTCCGGAAAGACCTGGAGCAAACTCGTTCAGAGAAAAAATGCATTGAGGAACAT GCAGATCGATACAAACAAGAAACAGAGCAGCTGGTATCAAATCTGAAGGAAGAAAATACTTTGCTGAAG CAAGAAAAAGAAGCCCTCAATCACCGCATCGTGCAGCAGGCTAAGGAGATGACAGAAACTATGGAGAAG AAGTTAGTAGAAGAAACGAAACAACTGGAACTCGACCTTAATGATGAAAGGCTGAGATATCAGAACCTT CTGAATGAGTTCAGTCGCCTGGAAGAAAGATATGATGACCTCAAGGAAGAGATGACCCTTATGGTGCAT GTGCCTAAGCCTGGACACAAGAGAACAGACTCCACCCACAGCAGCAACGAGTCTGAATATATCTTTAGC TCTGAAATTGCAGAAATGGAAGACATTCCATCAAGGACAGAGGAACCAAGTGAGAAGAAGGTACCTCTG GACATGTCATTGTTCCTTAAGCTCCAGAAGCGGGTCACAGAGCTGGAGCAGGAGAAGCAGGTGATGCAG GATGAGCTGGACCGCAAGGAGGAGCAGGTGCTCCGCAGCAAGGCCAAGGAAGAAGAAAGACCACAAATT AGAGGTGCAGAACTGGAATATGAGTCACTCAAGCGTCAAGAACTAGAATCAGAAAACAAAAAACTGAAG AATGAGCTAAATGAGTTGCGCAAGGCCCTCAGTGAGAAAAGTGCCCCAGAGGTGACCGCCCCAGGTGCA CCTGCCTACCGTGTCCTCATGGAGCAGCTGACCTCTGTGAGCGAGGAGCTTGATGTCCGCAAGGAGGAA GTCCTCATCTTAAGGTCTCAACTGGTGAGCCAGAAAGAGGCCATCCAACCCAAGGATGACAAGAATACA ATGACAGATTCCACAATACTTTTGGAAGATGTACAAAAAATGAAAGATAAAGGTGAAATAGCACAAGCA TACATTGGTTTGAAAGAAACAAATAGATCATCTGCTCTGGATTACCATGAGTTGAATGAGGATGGAGAG CTGTGGCTGGTTTATGAAGGGTTAAAACAAGCCAATAGGCTCCTGGAATCCCAGCTGCAGTCACAGAAG AGGAGCCATGAGAATGAGGCCGAGGCCCTCCGTGGGGAGATCCAGAGCCTGAAGGAGGAGAACAACCGA CAGCAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCAGAGGCCCGCATTGAGGCCAGCCTGCAGCAC GAGATCACCCGGCTGACCAACGAAAACTTGGATTTGATGGAACAACTTGAAAAACAGGATAAGACGGTC CGTAAACTGAAAAAACAACTGAAAGTATTTGCCAAAAAAATTGGCGAACTAGAAGTGGGCCAGATGGAG AACATATCCCCAGGACAGATCATTGATGAACCCATCCGACCAGTCAACATTCCCAGGAAAGAAAAGGAT TTCCAAGGGATGCTGGAATACAAGAAGGAGGATGAGCAAAAACTTGTTAAGAACCTGATTCTGGAACTG AAGCCACGTGGTGTAGCAGTCAATTTGATTCCAGGATTACCGGCATATATCCTGTTCATGTGTGTTCGA CATGCTGACTACCTGAATGATGATCAGAAAGTAAGGTCGTTGCTAACATCAACAATTAACAGCATCAAA AAAGTATTGAAGAAAAGAGGTGATGATTTTGAAACCGTCTCCTTCTGGCTCTCTAACACATGCCGATTT TTGCACTGCTTGAAACAGTACAGTGGAGAAGAGGGCTTTATGAAGCACAACACATCTCGCCAGAATGAA CACTGCCTCACCAATTTTGACCTGGCTGAGTATCGGCAGGTGCTGAGTGACTTGGCCATTCAGATCTAC CAGCAGCTCGTGCGGGTGTTAGAGAACATCCTTCAGCCAATGATTGTCTCAGGCATGCTGGAACATGAA ACGATTCAGGGCGTGTCTGGGGTGAAGCCCACAGGGTTGAGAAAGCGAACCTCCAGTATCGCCGATGAG GGCACCTACACACTGGACTCCATCCTCCGGCAGCTCAACTCCTTCCACTCGGTCATGTGTCAGCATGGC ATGGACCCTGAACTGATCAAGCAGGTGGTCAAGCAGATGTTCTACATCATAGGGGCCATCACCCTGAAC AACCTTCTCCTGCGGAAGGACATGTGCTCCTGGAGTAAAGGCATGCAGATCAGGTACAATGTCAGTCAA CTGGAAGAATGGCTGCGTGACAAGAATCTGATGAATAGTGGGGCTAAAGAAACCCTGGAACCTCTCATT CAGGCTGCTCAACTTTTGCAAGTGAAAAAGAAAACAGATGATGATGCAGAAGCCATTTGTTCTATGTGC AATGCTTTAACTACTGCCCAGATTGTGAAAGTGTTGAATTTGTATACTCCAGTTAATGAGTTTGAAGAA AGAGTCTCTGTGTCGTTCATTCGTACTATACAGATGCGTTTACGAGACAGGAAAGACTCTCCCCAGCTG CTCATGGATGCTAAACACATCTTTCCTGTCACCTTTCCTTTCAACCCATCTTCCCTCGCACTAGAAACC ATCCAGATTCCAGCCAGCCTCGGCCTGGGCTTTATTTCACGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC218817 Blue=ORF Red=Cloning site Green=Tag(s) MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILV GENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPH IFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLTSNPIMESIG NAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKM LRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDA DSCTIPPKHEPLCIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNW IVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIP WTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQH FADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTK GRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKLPFTFDEKRAVQQLRACGVLETI RISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLE KLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMY VVRRRYKIRRAATIVLQSYLRGFLARNRYRKILRGHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFR RMMAKRELKKLKIEARSVERYKKLRIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRS DLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLK QEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVH VPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQ DELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGA PAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQA YIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNR QQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQME NISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVR HADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNE HCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADE GTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQ LEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEE RVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_000259
ORF Size	5565 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000259.1, NP_000250.1
RefSeq Size	6401 bp
RefSeq ORF	5568 bp
Locus ID	4644
UniProt ID	Q9Y4I1
MW	215.3 kDa
Gene Summary	This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

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