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KCNH1 (NM_172362) Human Tagged ORF Clone
CAT＃: RC218681
- TrueORF®
KCNH1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1
ORF Plasmid: tGFP
"NM_172362" in other vectors (6)
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CNY 9,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EAG; EAG1; h-eag; hEAG; hEAG1; Kv10.1; TMBTS; ZLS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218681 representing NM_172362
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGCTGGGGGCAGGAGGGGACTAGTGGCCCCTCAAAACACGTTTCTGGAGAATATTGTTCGGC GGTCCAATGATACTAATTTTGTGTTGGGGAATGCTCAGATAGTGGACTGGCCTATTGTGTACAGCAATGA TGGATTTTGCAAGCTGTCTGGCTATCACAGGGCAGAAGTGATGCAAAAAAGCAGCACCTGCAGTTTTATG TATGGGGAGCTGACTGATAAAGACACGATTGAAAAAGTGCGGCAAACATTTGAGAACTATGAGATGAATT CCTTTGAAATTCTGATGTACAAGAAGAACAGGACACCTGTGTGGTTCTTTGTGAAAATTGCTCCAATTCG AAACGAACAGGATAAAGTGGTTTTATTTCTTTGCACTTTCAGTGACATAACAGCTTTCAAACAGCCAATT GAGGATGATTCATGTAAAGGCTGGGGGAAGTTTGCTCGGCTGACAAGAGCACTGACAAGCAGCAGGGGTG TCCTGCAGCAGCTGGCTCCAAGCGTGCAAAAAGGCGAGAATGTCCACAAGCACTCCCGCCTGGCAGAGGT CCTACAGCTGGGCTCAGACATCCTTCCCCAGTACAAGCAAGAGGCACCAAAGACTCCCCCTCACATCATC TTACATTATTGTGTTTTTAAGACCACGTGGGATTGGATCATCTTGATCTTGACCTTCTATACAGCCATCT TGGTCCCTTATAATGTCTCCTTCAAAACCAGGCAGAATAATGTGGCCTGGCTGGTTGTTGATAGCATCGT GGATGTTATCTTTTTGGTGGACATTGTGCTCAATTTTCATACCACCTTTGTTGGACCAGCAGGGGAGGTG ATTTCTGACCCCAAACTTATCCGCATGAACTACCTGAAGACGTGGTTTGTGATTGACCTTCTGTCCTGTT TGCCATATGATGTCATCAACGCTTTTGAGAACGTGGATGAGGTTAGTGCCTTTATGGGTGATCCAGGGAA GATTGGTTTTGCTGATCAGATTCCACCACCACTGGAGGGGAGAGAGAGTCAGGGCATCAGCAGCCTGTTC AGCTCTCTAAAAGTTGTCCGGCTGCTCCGTCTTGGGCGAGTGGCCCGTAAGCTGGACCACTACATTGAAT ATGGAGCTGCTGTGCTGGTCCTGCTGGTGTGTGTGTTTGGGCTGGCTGCACACTGGATGGCCTGCATCTG GTACAGCATTGGGGACTATGAGATCTTTGACGAGGACACCAAGACAATCCGCAACAACAGCTGGCTGTAC CAACTAGCGATGGACATTGGCACCCCTTACCAGTTTAATGGGTCTGGCTCAGGGAAGTGGGAAGGTGGTC CCAGCAAGAATTCTGTCTACATCTCCTCGTTGTATTTCACAATGACCAGCCTCACCAGTGTGGGCTTTGG GAACATCGCCCCATCCACAGACATTGAGAAGATCTTTGCAGTGGCCATCATGATGATTGGCTCACTTCTC TATGCCACCATCTTCGGGAATGTGACGACTATTTTCCAACAGATGTATGCCAACACCAACAGATACCATG AGATGCTCAACAGTGTTCGGGACTTCCTGAAGCTCTACCAGGTGCCAAAAGGATTGAGTGAGCGAGTAAT GGATTATATTGTGTCCACTTGGTCCATGTCCAGAGGCATTGACACAGAGAAGGTCCTGCAGATCTGCCCC AAGGACATGAGAGCCGACATCTGCGTGCACCTGAACCGCAAGGTGTTCAAGGAGCACCCGGCCTTCCGGC TGGCCAGTGATGGCTGCCTCCGGGCACTGGCCATGGAGTTCCAGACGGTGCACTGTGCCCCAGGGGACCT CATCTACCATGCAGGAGAGAGCGTTGACAGCCTCTGCTTTGTGGTTTCTGGCTCCCTGGAGGTGATCCAA GATGATGAGGTGGTGGCCATTCTAGGAAAAGGAGACGTGTTTGGAGATGTGTTCTGGAAGGAAGCCACCC TTGCCCAGTCCTGTGCCAATGTTAGGGCCTTGACCTACTGTGATCTGCATGTGATCAAGCGGGATGCCCT GCAGAAAGTGCTGGAATTCTACACGGCCTTCTCCCATTCCTTCTCCCGGAACCTGATTCTGACGTACAAC TTGAGGAAGAGGATTGTGTTCCGGAAGATCAGCGATGTGAAACGTGAAGAGGAAGAACGCATGAAACGAA AGAATGAGGCCCCCCTGATCTTGCCCCCGGACCACCCTGTCCGGCGCCTCTTCCAGAGATTCCGACAGCA GAAAGAGGCCAGGCTGGCAGCTGAGAGAGGGGGCCGGGACCTGGATGACCTAGATGTGGAGAAGGGCAAT GTCCTTACAGAGCATGCCTCCGCCAACCACAGCCTCGTGAAGGCCAGCGTGGTCACCGTGCGTGAGAGTC CTGCCACGCCCGTATCCTTCCAGGCAGCCTCCACCTCCGGGGTGCCAGACCACGCAAAGCTACAGGCGCC AGGGTCCGAGTGCCTGGGCCCCAAGGGGGGCGGGGGCGATTGTGCCAAGCGCAAAAGCTGGGCCCGCTTC AAAGATGCTTGCGGGAAGAGTGAGGACTGGAACAAGGTGTCCAAGGCTGAGTCGATGGAGACACTTCCCG AGAGGACAAAAGCGTCAGGCGAGGCCACACTGAAGAAGACAGACTCGTGTGACAGTGGCATCACCAAGAG CGACTTGCGCCTGGACAACGTGGGTGAGGCCAGGAGTCCCCAGGATCGGAGTCCCATCCTGGCAGAGGTC AAGCATTCGTTCTACCCCATCCCTGAGCAGACGCTGCAGGCCACAGTCCTGGAGGTGAGGCACGAGCTGA AGGAGGACATCAAGGCCTTAAACGCCAAAATGACCAATATTGAGAAACAGCTCTCTGAGATACTCAGGAT ATTAACTTCCAGAAGATCCTCTCAGTCTCCTCAGGAGTTGTTTGAAATATCGAGGCCACAGTCCCCAGAA TCAGAGAGAGACATTTTTGGAGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218681 representing NM_172362
Red=Cloning site Green=Tags(s) MTMAGGRRGLVAPQNTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFM YGELTDKDTIEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPI EDDSCKGWGKFARLTRALTSSRGVLQQLAPSVQKGENVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHII LHYCVFKTTWDWIILILTFYTAILVPYNVSFKTRQNNVAWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEV ISDPKLIRMNYLKTWFVIDLLSCLPYDVINAFENVDEVSAFMGDPGKIGFADQIPPPLEGRESQGISSLF SSLKVVRLLRLGRVARKLDHYIEYGAAVLVLLVCVFGLAAHWMACIWYSIGDYEIFDEDTKTIRNNSWLY QLAMDIGTPYQFNGSGSGKWEGGPSKNSVYISSLYFTMTSLTSVGFGNIAPSTDIEKIFAVAIMMIGSLL YATIFGNVTTIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTEKVLQICP KDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVSGSLEVIQ DDEVVAILGKGDVFGDVFWKEATLAQSCANVRALTYCDLHVIKRDALQKVLEFYTAFSHSFSRNLILTYN LRKRIVFRKISDVKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGN VLTEHASANHSLVKASVVTVRESPATPVSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARF KDACGKSEDWNKVSKAESMETLPERTKASGEATLKKTDSCDSGITKSDLRLDNVGEARSPQDRSPILAEV KHSFYPIPEQTLQATVLEVRHELKEDIKALNAKMTNIEKQLSEILRILTSRRSSQSPQELFEISRPQSPE SERDIFGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172362 |
| ORF Size | 2967 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172362.3 |
| RefSeq Size | 3208 bp |
| RefSeq ORF | 2970 bp |
| Locus ID | 3756 |
| UniProt ID | O95259 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 111.2 kDa |
| Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218681L1 | Lenti-ORF clone of KCNH1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1 |
CNY 13,264.00 |
|
| RC218681L2 | Lenti-ORF clone of KCNH1 (mGFP-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1 |
CNY 11,690.00 |
|
| RC218681L3 | Lenti-ORF clone of KCNH1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1 |
CNY 11,690.00 |
|
| RC218681L4 | Lenti-ORF clone of KCNH1 (mGFP-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1 |
CNY 11,690.00 |
|
| RG218681 | KCNH1 (tGFP-tagged) - Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1 |
CNY 12,464.00 |
|
| SC306758 | KCNH1 (untagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1 |
CNY 19,000.00 |
